Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPW86_RS14650 Genome accession   NZ_CP046742
Coordinates   1870340..1871377 (+) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae strain 3523-03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1865340..1876377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPW86_RS14615 hemW 1865515..1866690 (-) 1176 WP_123011309.1 radical SAM family heme chaperone HemW -
  GPW86_RS14620 - 1866684..1867286 (-) 603 WP_000725022.1 XTP/dITP diphosphatase -
  GPW86_RS14625 - 1867356..1867786 (-) 431 Protein_1608 DUF4426 domain-containing protein -
  GPW86_RS14630 yggU 1867832..1868122 (-) 291 WP_123011310.1 DUF167 family protein YggU -
  GPW86_RS14635 - 1868122..1868679 (-) 558 WP_001087261.1 YggT family protein -
  GPW86_RS14640 proC 1868733..1869551 (-) 819 WP_000437941.1 pyrroline-5-carboxylate reductase -
  GPW86_RS14645 - 1869604..1870314 (-) 711 WP_001256648.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPW86_RS14650 pilT 1870340..1871377 (+) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  GPW86_RS14655 pilU 1871392..1872498 (+) 1107 WP_000422572.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GPW86_RS14660 - 1872616..1873413 (+) 798 WP_184476170.1 helix-turn-helix transcriptional regulator -
  GPW86_RS14665 tyrS 1873522..1874802 (+) 1281 WP_148039146.1 tyrosine--tRNA ligase -
  GPW86_RS14670 ruvX 1874840..1875262 (-) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  GPW86_RS14675 - 1875343..1875906 (-) 564 WP_001054771.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=406973 GPW86_RS14650 WP_000350195.1 1870340..1871377(+) (pilT) [Vibrio cholerae strain 3523-03]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=406973 GPW86_RS14650 WP_000350195.1 1870340..1871377(+) (pilT) [Vibrio cholerae strain 3523-03]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCAGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGTTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilT Vibrio cholerae strain A1552

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment