Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GPJ68_RS20595 Genome accession   NZ_CP046673
Coordinates   4245639..4246619 (-) Length   326 a.a.
NCBI ID   WP_047357838.1    Uniprot ID   -
Organism   Citrobacter sp. 172116965     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4240639..4251619
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GPJ68_RS20565 (GPJ68_20560) - 4241420..4241917 (+) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -
  GPJ68_RS20570 (GPJ68_20565) endA 4242012..4242719 (+) 708 WP_019077649.1 deoxyribonuclease I -
  GPJ68_RS20575 (GPJ68_20570) rsmE 4242795..4243526 (+) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GPJ68_RS20580 (GPJ68_20575) gshB 4243539..4244486 (+) 948 WP_016154394.1 glutathione synthase -
  GPJ68_RS20585 (GPJ68_20580) - 4244663..4245226 (+) 564 WP_016154395.1 YqgE/AlgH family protein -
  GPJ68_RS20590 (GPJ68_20585) ruvX 4245226..4245642 (+) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  GPJ68_RS20595 (GPJ68_20590) pilT 4245639..4246619 (-) 981 WP_047357838.1 type IV pilus twitching motility protein PilT Machinery gene
  GPJ68_RS20600 (GPJ68_20595) - 4246637..4246807 (+) 171 Protein_4075 YggS family pyridoxal phosphate enzyme -
  GPJ68_RS20605 (GPJ68_20600) - 4246813..4247232 (-) 420 WP_075847428.1 DUF3828 domain-containing protein -
  GPJ68_RS20610 (GPJ68_20605) - 4247232..4247669 (-) 438 WP_370461154.1 hypothetical protein -
  GPJ68_RS20615 - 4247942..4248067 (-) 126 Protein_4078 type IV pili twitching motility protein PilT -
  GPJ68_RS20620 (GPJ68_20610) - 4248085..4248789 (+) 705 WP_101700213.1 YggS family pyridoxal phosphate-dependent enzyme -
  GPJ68_RS20625 (GPJ68_20615) - 4248808..4249374 (+) 567 WP_016154401.1 YggT family protein -
  GPJ68_RS20630 (GPJ68_20620) yggU 4249371..4249661 (+) 291 WP_005123286.1 DUF167 family protein YggU -
  GPJ68_RS20635 (GPJ68_20625) - 4249669..4250262 (+) 594 WP_016154402.1 XTP/dITP diphosphatase -
  GPJ68_RS20640 (GPJ68_20630) hemW 4250255..4251391 (+) 1137 WP_104651967.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35910.98 Da        Isoelectric Point: 6.1727

>NTDB_id=406637 GPJ68_RS20595 WP_047357838.1 4245639..4246619(-) (pilT) [Citrobacter sp. 172116965]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLMCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=406637 GPJ68_RS20595 WP_047357838.1 4245639..4246619(-) (pilT) [Citrobacter sp. 172116965]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGCACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACTCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCGTCAACACGCCCGCCGTGGGCAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(59-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.341

100

0.466
  pilT Neisseria gonorrhoeae MS11

46.037

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.728

99.387

0.365

Multiple sequence alignment