Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THENA_RS01540 Genome accession   NC_015499
Coordinates   320666..321724 (+) Length   352 a.a.
NCBI ID   WP_013755681.1    Uniprot ID   -
Organism   Thermodesulfobium narugense DSM 14796     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 315666..326724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THENA_RS01530 (Thena_0303) - 317521..318801 (-) 1281 WP_013755679.1 amidohydrolase -
  THENA_RS01535 (Thena_0304) - 318933..320657 (+) 1725 WP_013755680.1 GspE/PulE family protein -
  THENA_RS01540 (Thena_0305) pilT 320666..321724 (+) 1059 WP_013755681.1 type IV pilus twitching motility protein PilT Machinery gene
  THENA_RS01545 (Thena_0306) - 321724..322968 (+) 1245 WP_013755682.1 type II secretion system F family protein -
  THENA_RS10175 (Thena_0307) - 323117..323515 (+) 399 WP_013755683.1 type IV pilin protein -
  THENA_RS01555 (Thena_0308) - 323585..323986 (+) 402 WP_013755684.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  THENA_RS01560 (Thena_0309) - 323983..324582 (-) 600 WP_041437893.1 hypothetical protein -
  THENA_RS01565 (Thena_0310) - 324688..325065 (-) 378 WP_013755686.1 hypothetical protein -
  THENA_RS01570 (Thena_0311) - 325109..325714 (-) 606 WP_013755687.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38988.03 Da        Isoelectric Point: 7.0685

>NTDB_id=40649 THENA_RS01540 WP_013755681.1 320666..321724(+) (pilT) [Thermodesulfobium narugense DSM 14796]
MEITELLEKATQMNASDIHISVGIPPVIRREGKLIRLEEYGNITPKIAFELIFSMLSDYQKKKIEEELDIDFSYGISGVG
RFRVNVFKQRGVWGAALRLIPWDIPSLETLGLPPIIAEFAKLVRGLVLVTGPTGSGKSTTLASLVNIINRTRECHIITIE
DPIEYLHSHKKSIVDQREVGNDTKSFARAVRASLREDPDVILVGEMRDLETIAAAITAAETGHLVFSTLHTNDAPQTIDR
IIDVFPTNQQQQIRTQLSTALQGVVSQALLPKIGGGRVVATEVMIATPAVRNLIREGKTSQLYSVIQTSGRYGMQQLDSS
LKDLVLRGKVSFEDAFTVAVNKEEFMRLVGRL

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=40649 THENA_RS01540 WP_013755681.1 320666..321724(+) (pilT) [Thermodesulfobium narugense DSM 14796]
ATGGAGATAACTGAACTTCTTGAAAAGGCTACTCAAATGAATGCGAGTGACATTCACATCTCTGTGGGAATACCCCCTGT
AATAAGAAGAGAGGGAAAACTAATAAGATTGGAAGAATATGGAAATATTACTCCAAAGATCGCATTTGAGCTTATTTTCT
CTATGCTTTCAGATTACCAGAAAAAGAAAATAGAGGAAGAGTTAGATATTGACTTTTCCTACGGTATATCTGGAGTTGGA
AGATTCAGGGTAAACGTGTTTAAACAAAGAGGAGTATGGGGAGCAGCGCTAAGGCTTATTCCATGGGATATTCCAAGCCT
AGAAACTCTGGGACTACCACCTATAATAGCTGAATTTGCAAAACTGGTAAGAGGGCTTGTCCTTGTAACAGGTCCTACTG
GCTCAGGGAAATCTACCACGCTTGCCTCTCTTGTAAACATAATAAACAGAACAAGGGAGTGTCATATTATTACTATTGAA
GACCCTATTGAATACCTTCACTCTCACAAGAAATCTATTGTAGATCAAAGAGAGGTTGGAAATGACACTAAGTCTTTTGC
TAGAGCTGTTAGGGCATCCTTAAGAGAGGACCCTGACGTAATACTAGTAGGCGAGATGAGGGACCTCGAAACTATTGCTG
CAGCTATCACAGCTGCTGAAACAGGGCATTTGGTGTTCTCAACCCTTCACACTAACGATGCACCACAAACTATAGACAGA
ATTATAGATGTCTTCCCAACCAATCAGCAGCAGCAAATAAGGACTCAGCTTTCCACTGCCCTTCAAGGAGTGGTATCTCA
GGCACTTTTGCCAAAGATTGGCGGTGGTAGAGTGGTAGCAACGGAGGTAATGATTGCAACTCCTGCTGTAAGAAACCTTA
TTAGAGAGGGCAAGACGTCCCAGCTTTATTCTGTAATACAGACCTCCGGAAGATACGGCATGCAGCAGTTAGACTCATCT
CTTAAAGATCTAGTTCTTCGAGGCAAGGTATCCTTTGAGGATGCTTTTACCGTAGCTGTGAACAAGGAAGAGTTCATGCG
TCTGGTAGGTAGGTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.736

98.864

0.531
  pilT Acinetobacter baylyi ADP1

53.913

98.011

0.528
  pilT Legionella pneumophila strain ERS1305867

53.448

98.864

0.528
  pilT Legionella pneumophila strain Lp02

53.448

98.864

0.528
  pilT Pseudomonas stutzeri DSM 10701

53.333

98.011

0.523
  pilT Acinetobacter nosocomialis M2

52.601

98.295

0.517
  pilT Acinetobacter baumannii D1279779

52.601

98.295

0.517
  pilT Acinetobacter baumannii strain A118

52.601

98.295

0.517
  pilT Neisseria meningitidis 8013

50.425

100

0.506
  pilT Neisseria gonorrhoeae MS11

50.142

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.994

94.886

0.503
  pilT Vibrio cholerae strain A1552

52.994

94.886

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.571

99.432

0.503
  pilU Pseudomonas stutzeri DSM 10701

40.51

100

0.406
  pilU Vibrio cholerae strain A1552

41.765

96.591

0.403
  pilU Acinetobacter baylyi ADP1

41.056

96.875

0.398

Multiple sequence alignment