Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GM661_RS11510 Genome accession   NZ_CP046640
Coordinates   2386483..2387553 (-) Length   356 a.a.
NCBI ID   WP_407929666.1    Uniprot ID   A0A8A7KJH4
Organism   Iocasia fonsfrigidae strain NS-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2381483..2392553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GM661_RS11480 (GM661_11510) - 2382392..2382931 (-) 540 WP_230866966.1 type II secretion system protein -
  GM661_RS11485 (GM661_11515) - 2382928..2383347 (-) 420 WP_230866967.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  GM661_RS11490 (GM661_11520) - 2383364..2383783 (-) 420 WP_125990756.1 Tfp pilus assembly protein FimT/FimU -
  GM661_RS11495 (GM661_11525) - 2383807..2384553 (-) 747 WP_230866968.1 A24 family peptidase -
  GM661_RS11500 (GM661_11530) - 2384669..2385115 (-) 447 WP_230866969.1 type II secretion system protein -
  GM661_RS11505 (GM661_11535) - 2385246..2386457 (-) 1212 WP_230866970.1 type II secretion system F family protein -
  GM661_RS11510 (GM661_11540) pilT 2386483..2387553 (-) 1071 WP_407929666.1 type IV pilus twitching motility protein PilT Machinery gene
  GM661_RS11515 (GM661_11545) gspE 2387576..2389252 (-) 1677 WP_230866972.1 type II secretion system ATPase GspE -
  GM661_RS11520 (GM661_11550) - 2389411..2390274 (-) 864 WP_230866973.1 shikimate dehydrogenase -
  GM661_RS11525 (GM661_11555) - 2390267..2391157 (-) 891 WP_230866974.1 DMT family transporter -
  GM661_RS11530 (GM661_11560) - 2391173..2391865 (-) 693 WP_125990762.1 Crp/Fnr family transcriptional regulator -
  GM661_RS11535 (GM661_11565) - 2391875..2392330 (-) 456 WP_125992228.1 Fur family transcriptional regulator -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39733.70 Da        Isoelectric Point: 6.4163

>NTDB_id=406350 GM661_RS11510 WP_407929666.1 2386483..2387553(-) (pilT) [Iocasia fonsfrigidae strain NS-1]
MDIIGLLKSVVEDANISDLHLTVNSQAVVRTTGVLRAYEQYSHKFTFADLIEICRYLMDEDQWKTFQEQGELDFSYSVPG
FSRFRVNAYRQRGAVSLALRIIPTEIPTIDDLGLPEILKKMAVQRRGLVLCTGPTGSGKSTTLASMINEINQERKCHILT
LEDPIEYLHQHNNSIVHQREVGIDTRSFAAGLRAALRQDPDVILVGEMRDLETISIALESAETGHLVLATLHTTDAPKTV
DRIIDVFPAHQQQQVRIQLSSVLNGIIAQQLLPRADMEGMVAGLEVLIGTPAVRNIIREGKSSQLESIMQTGAKYGMIVM
NNYLISLYEKGLISMETAVRRSTNPEYIKKGITRFG

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=406350 GM661_RS11510 WP_407929666.1 2386483..2387553(-) (pilT) [Iocasia fonsfrigidae strain NS-1]
CTGGATATAATAGGATTATTAAAGAGTGTTGTTGAAGATGCTAATATTTCTGATCTCCATTTAACGGTAAATTCTCAGGC
TGTTGTCAGGACTACCGGGGTATTAAGGGCTTATGAACAATATTCTCATAAATTTACTTTCGCTGACCTGATTGAGATAT
GTCGGTATCTGATGGATGAGGATCAGTGGAAGACCTTTCAGGAACAAGGGGAACTTGATTTTTCTTATAGTGTTCCCGGT
TTTTCCCGTTTCAGGGTAAATGCCTATCGCCAGAGGGGGGCTGTTAGTCTGGCCCTGCGAATTATTCCGACAGAGATACC
GACAATTGATGACCTGGGGTTGCCGGAAATACTCAAAAAAATGGCGGTTCAGCGCAGAGGCTTGGTCTTATGTACTGGGC
CAACCGGTAGTGGTAAATCAACTACCCTGGCCTCAATGATCAATGAGATTAATCAAGAGAGGAAATGTCATATCCTGACC
CTGGAGGACCCGATTGAATACCTCCATCAACATAATAATTCAATTGTTCATCAAAGGGAGGTAGGGATTGATACCAGATC
ATTTGCTGCAGGACTGCGGGCTGCCCTGCGTCAGGACCCAGATGTAATATTGGTAGGTGAGATGCGTGACCTGGAGACTA
TTTCTATTGCTCTGGAATCTGCTGAGACCGGTCATCTGGTGCTGGCTACTCTCCATACAACTGATGCCCCCAAGACTGTT
GATAGAATTATTGATGTTTTTCCAGCTCATCAACAACAGCAGGTTCGAATCCAGTTATCTTCTGTACTTAACGGGATTAT
TGCTCAACAGCTTTTGCCCCGGGCAGATATGGAGGGAATGGTGGCTGGGCTGGAGGTGTTAATTGGTACTCCAGCTGTAC
GCAATATTATTCGCGAGGGTAAAAGTTCACAACTGGAATCAATTATGCAGACAGGTGCGAAATACGGTATGATAGTGATG
AATAATTACCTGATATCACTTTACGAAAAGGGATTGATCAGTATGGAAACTGCAGTCAGGCGTTCTACTAATCCTGAATA
TATCAAAAAGGGCATCACCAGGTTTGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8A7KJH4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.429

98.315

0.486
  pilT Legionella pneumophila strain Lp02

49.133

97.191

0.478
  pilT Legionella pneumophila strain ERS1305867

49.133

97.191

0.478
  pilT Pseudomonas stutzeri DSM 10701

48.276

97.753

0.472
  pilT Pseudomonas aeruginosa PAK

47.989

97.753

0.469
  pilT Acinetobacter nosocomialis M2

47.989

97.753

0.469
  pilT Acinetobacter baumannii D1279779

47.989

97.753

0.469
  pilT Acinetobacter baumannii strain A118

47.989

97.753

0.469
  pilT Vibrio cholerae strain A1552

49.702

94.382

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.702

94.382

0.469
  pilT Acinetobacter baylyi ADP1

47.11

97.191

0.458
  pilT Neisseria meningitidis 8013

49.096

93.258

0.458
  pilT Neisseria gonorrhoeae MS11

48.795

93.258

0.455
  pilU Pseudomonas stutzeri DSM 10701

44.838

95.225

0.427
  pilU Acinetobacter baylyi ADP1

39.429

98.315

0.388
  pilU Vibrio cholerae strain A1552

41.486

90.73

0.376

Multiple sequence alignment