Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GOY17_RS03385 Genome accession   NZ_CP046603
Coordinates   741856..742968 (-) Length   370 a.a.
NCBI ID   WP_115841327.1    Uniprot ID   A0A3D8VHC5
Organism   Lysobacter soli strain XL170     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 736856..747968
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GOY17_RS03355 (GOY17_03355) - 737436..738629 (-) 1194 WP_157029330.1 (Fe-S)-binding protein -
  GOY17_RS03360 (GOY17_03360) rplM 738812..739240 (+) 429 WP_115841323.1 50S ribosomal protein L13 -
  GOY17_RS03365 (GOY17_03365) rpsI 739267..739659 (+) 393 WP_115841324.1 30S ribosomal protein S9 -
  GOY17_RS03375 (GOY17_03375) - 739975..740964 (+) 990 WP_157029331.1 fumarylacetoacetate hydrolase family protein -
  GOY17_RS03380 (GOY17_03380) maiA 741028..741693 (+) 666 WP_115841326.1 maleylacetoacetate isomerase -
  GOY17_RS03385 (GOY17_03385) pilU 741856..742968 (-) 1113 WP_115841327.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GOY17_RS03390 (GOY17_03390) - 743198..743455 (+) 258 WP_232338514.1 DUF4398 domain-containing protein -
  GOY17_RS03395 (GOY17_03395) - 743452..744336 (+) 885 WP_198173806.1 OmpA family protein -
  GOY17_RS03400 (GOY17_03400) - 744725..744943 (+) 219 WP_115841330.1 YdcH family protein -
  GOY17_RS03405 (GOY17_03405) - 745177..746553 (+) 1377 WP_157029332.1 pyridoxal-phosphate dependent enzyme -
  GOY17_RS03410 (GOY17_03410) - 746553..747497 (+) 945 WP_157029333.1 2OG-Fe(II) oxygenase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41126.43 Da        Isoelectric Point: 5.8003

>NTDB_id=405986 GOY17_RS03385 WP_115841327.1 741856..742968(-) (pilU) [Lysobacter soli strain XL170]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPLFERDLELNMALALPEAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSNISGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVVGTDGRRVPAAEVLINTPHIRDLMRRGQVHEIKQAMEESLEEGMESFDQCL
FRLYKEGRIDMEEALKAADSREGLSLKFRLSEGSSGEHDPYAEIYDTATQ

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=405986 GOY17_RS03385 WP_115841327.1 741856..742968(-) (pilU) [Lysobacter soli strain XL170]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAAAAGAATGCGTCGGACATGTTCCTGACGACCGGCGCACCGGTTTA
TATCAAGGTCGAAGGCAAGCTCTACCCCCTCGGCAATACCGGCCTGCCCCCCGGCATGGTCAAGAAGATCGCCTATTCGT
TGATGGACGAGGGCCAGGTTCCGCTTTTCGAGCGTGACCTCGAGCTGAACATGGCGCTCGCCCTGCCCGAAGCCGGCCGC
TTCCGCATCAACGTCTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCGCCATCCGCAGCGTGATCCCGTCGATCGA
GGAACTGCAGCTGCCGCAGGTGCTGAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTCGCGTCGATGATCGACTACCGCAACAGCAACATCTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGAAGTCGATCGTGAACCAGCGCGAGGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTCATCGGCGAAATCCTCGATGCCACGACGATGGAAGCGG
CGATCGCGTTCGCCGAAACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACGCTGGAACGCATC
CTCAACTTCTTCCCGGAAGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTCAACCTGCGCGCGGTGGTCAGCCAGCG
CCTGGTCGTCGGCACCGATGGCCGCCGCGTGCCGGCGGCGGAAGTGCTGATCAACACGCCGCACATCCGCGACCTCATGC
GCCGCGGCCAGGTGCATGAGATCAAGCAAGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGTCCTTCGACCAGTGCCTG
TTCCGCCTCTACAAGGAAGGCCGCATCGACATGGAAGAGGCGCTCAAGGCCGCCGACTCCCGCGAAGGCCTCTCGCTCAA
GTTCCGCCTCTCGGAAGGCTCGAGCGGCGAACACGATCCGTACGCGGAAATCTACGACACGGCGACGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3D8VHC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

97.027

0.505
  pilU Acinetobacter baylyi ADP1

48.87

95.676

0.468
  pilU Vibrio cholerae strain A1552

48.023

95.676

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.251

96.757

0.438
  pilT Acinetobacter baumannii strain A118

41.57

92.973

0.386
  pilT Acinetobacter nosocomialis M2

41.57

92.973

0.386
  pilT Acinetobacter baumannii D1279779

41.57

92.973

0.386
  pilT Pseudomonas aeruginosa PAK

42.012

91.351

0.384
  pilT Acinetobacter baylyi ADP1

41.279

92.973

0.384
  pilT Pseudomonas stutzeri DSM 10701

40.698

92.973

0.378
  pilT Legionella pneumophila strain Lp02

40.828

91.351

0.373
  pilT Legionella pneumophila strain ERS1305867

40.828

91.351

0.373
  pilT Neisseria gonorrhoeae MS11

39.769

93.784

0.373
  pilT Neisseria meningitidis 8013

39.769

93.784

0.373
  pilT Vibrio cholerae strain A1552

40.541

90

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.541

90

0.365

Multiple sequence alignment