Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNG29_RS19060 Genome accession   NZ_CP046557
Coordinates   4004715..4005698 (-) Length   327 a.a.
NCBI ID   WP_156655213.1    Uniprot ID   -
Organism   Leclercia sp. Colony189 strain 1106151     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3999715..4010698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNG29_RS19030 (GNG29_19030) endA 4000267..4000974 (+) 708 WP_156655211.1 deoxyribonuclease I -
  GNG29_RS19035 (GNG29_19035) rsmE 4001065..4001796 (+) 732 WP_156655212.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GNG29_RS19040 (GNG29_19040) gshB 4001816..4002763 (+) 948 WP_114316823.1 glutathione synthase -
  GNG29_RS19045 (GNG29_19045) - 4002919..4003479 (+) 561 WP_103176923.1 YqgE/AlgH family protein -
  GNG29_RS19050 (GNG29_19050) ruvX 4003479..4003895 (+) 417 WP_103176924.1 Holliday junction resolvase RuvX -
  GNG29_RS19055 (GNG29_19055) - 4003928..4004623 (-) 696 WP_103176925.1 transcriptional regulator -
  GNG29_RS19060 (GNG29_19060) pilT 4004715..4005698 (-) 984 WP_156655213.1 type IV pilus twitching motility protein PilT Machinery gene
  GNG29_RS19065 (GNG29_19065) - 4005716..4006417 (+) 702 WP_103176927.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNG29_RS19070 (GNG29_19070) - 4006439..4007005 (+) 567 WP_103176928.1 YggT family protein -
  GNG29_RS19075 (GNG29_19075) yggU 4007002..4007298 (+) 297 WP_103176929.1 DUF167 family protein YggU -
  GNG29_RS19080 (GNG29_19080) - 4007302..4007895 (+) 594 WP_103176930.1 XTP/dITP diphosphatase -
  GNG29_RS19085 (GNG29_19085) hemW 4007888..4009030 (+) 1143 WP_103176931.1 radical SAM family heme chaperone HemW -
  GNG29_RS19090 (GNG29_19090) - 4009219..4009938 (-) 720 WP_103176932.1 DUF2884 domain-containing protein -
  GNG29_RS19095 (GNG29_19095) - 4009991..4010317 (-) 327 WP_103176933.1 YggL family protein -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35908.24 Da        Isoelectric Point: 7.2502

>NTDB_id=405496 GNG29_RS19060 WP_156655213.1 4004715..4005698(-) (pilT) [Leclercia sp. Colony189 strain 1106151]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPEVAMLLKSWLSDEQQGAWCAQGQVDFAVTLASRQRLRA
SAFAHMQGCSLVLRLLPDACPQLRTLGAPRAIPELLAHDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVE
FIHQSKRCLVQQREIGLHCLSFADALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDA
FPAQEKEPVRRQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTWQLPGMIQTGQQAGMQNFEQSLAER
RAQGRLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=405496 GNG29_RS19060 WP_156655213.1 4004715..4005698(-) (pilT) [Leclercia sp. Colony189 strain 1106151]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGCAGTAACGCACCACCTCG
CTGGCGGCGGCGAGGGAAAATGGAATTTGCTCCCTTTCCGGGGCCGGAGGTCGCGATGCTGCTGAAAAGCTGGCTTAGCG
ACGAGCAGCAGGGTGCATGGTGTGCCCAAGGGCAGGTGGATTTTGCCGTTACGCTTGCGAGCAGGCAGCGGCTGCGGGCC
AGCGCCTTTGCGCATATGCAGGGGTGTTCTCTGGTGCTCCGCCTGCTTCCAGACGCGTGCCCGCAGTTGCGCACCCTGGG
AGCGCCGCGGGCGATACCCGAACTGCTGGCGCACGATAACGGTCTGATACTGGTGACCGGGGCGACAGGCAGCGGTAAAT
CCACCACCCTTGCGGCAATGGTCGATTTTCTCAATCACCATAGCGACGGGCATATCCTGACGCTCGAAGATCCGGTGGAA
TTTATCCATCAAAGCAAGCGTTGCCTGGTTCAGCAGCGGGAGATTGGCCTGCATTGCCTCTCCTTTGCCGACGCGCTGCG
GGCTGCATTACGTGAAGATCCCGATGTAATCCTGCTGGGCGAACTGCGTGACAGCGAGACGATACGTCTTGCGCTTACCG
CCGCAGAGACCGGGCATCTGGTGCTGGCGACGCTCCACACCCGGGGCGCCGCCCAGGCAGTGGAGAGGCTGGTGGATGCC
TTCCCCGCGCAGGAAAAAGAGCCGGTACGGAGACAGCTGGCAGGCAGCCTGCGCGCCGTGCTGGCGCAAAAACTCGAAAG
GGATAACCAGCAGGGGCGCGTCGCGCTGTTCGAATTACTGGTTAATACCCCTGCGGCAGCCAATTTAATCCGCGAAGGAA
AAACCTGGCAACTGCCCGGCATGATCCAGACCGGGCAGCAGGCTGGCATGCAAAACTTCGAGCAGAGCCTGGCTGAGCGC
AGGGCGCAGGGGCGATTGTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.495
  pilT Vibrio cholerae strain A1552

49.541

100

0.495
  pilT Neisseria meningitidis 8013

48.78

100

0.489
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.486
  pilT Legionella pneumophila strain ERS1305867

48.447

98.471

0.477
  pilT Legionella pneumophila strain Lp02

48.447

98.471

0.477
  pilT Pseudomonas stutzeri DSM 10701

46.97

100

0.474
  pilT Acinetobacter baumannii strain A118

46.364

100

0.468
  pilT Acinetobacter baumannii D1279779

46.364

100

0.468
  pilT Pseudomonas aeruginosa PAK

46.364

100

0.468
  pilT Acinetobacter baylyi ADP1

46.061

100

0.465
  pilT Acinetobacter nosocomialis M2

46.061

100

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.471

0.425
  pilU Vibrio cholerae strain A1552

39.697

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

38.369

100

0.388
  pilU Acinetobacter baylyi ADP1

37.231

99.388

0.37

Multiple sequence alignment