Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HXS77_RS03840 Genome accession   NZ_CP058355
Coordinates   792413..793393 (+) Length   326 a.a.
NCBI ID   WP_071999886.1    Uniprot ID   -
Organism   Escherichia coli strain AMSCJX03     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787413..798393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HXS77_RS03810 (HXS77_03810) yggM 787928..788935 (+) 1008 WP_000745217.1 DUF1202 family protein -
  HXS77_RS03815 (HXS77_03815) hemW 789090..790226 (-) 1137 WP_175068563.1 radical SAM family heme chaperone HemW -
  HXS77_RS03820 (HXS77_03820) rdgB 790219..790812 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  HXS77_RS03825 (HXS77_03825) yggU 790820..791110 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  HXS77_RS03830 (HXS77_03830) yggT 791107..791673 (-) 567 WP_032237483.1 osmotic shock tolerance protein YggT -
  HXS77_RS03835 (HXS77_03835) yggS 791691..792395 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  HXS77_RS03840 (HXS77_03840) pilT 792413..793393 (+) 981 WP_071999886.1 type IV pilus twitching motility protein PilT Machinery gene
  HXS77_RS03845 (HXS77_03845) ruvX 793568..793984 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  HXS77_RS03850 (HXS77_03850) yqgE 793984..794547 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  HXS77_RS03855 (HXS77_03855) gshB 794656..795606 (-) 951 WP_000593273.1 glutathione synthase -
  HXS77_RS03860 (HXS77_03860) rsmE 795619..796350 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HXS77_RS03865 (HXS77_03865) endA 796430..797137 (-) 708 WP_000286500.1 deoxyribonuclease I -
  HXS77_RS03870 (HXS77_03870) yggI 797232..797729 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35976.14 Da        Isoelectric Point: 5.7980

>NTDB_id=405388 HXS77_RS03840 WP_071999886.1 792413..793393(+) (pilT) [Escherichia coli strain AMSCJX03]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=405388 HXS77_RS03840 WP_071999886.1 792413..793393(+) (pilT) [Escherichia coli strain AMSCJX03]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362

Multiple sequence alignment