Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GJ703_RS18205 Genome accession   NZ_CP046450
Coordinates   3714999..3716051 (+) Length   350 a.a.
NCBI ID   WP_003386380.1    Uniprot ID   A0A2I4MRH7
Organism   Clostridium botulinum strain NCTC 13319     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3709999..3721051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ703_RS19440 - 3710831..3710986 (-) 156 WP_162265978.1 hypothetical protein -
  GJ703_RS19770 - 3711051..3711350 (+) 300 Protein_3391 hypothetical protein -
  GJ703_RS18175 (GJ703_03483) - 3711557..3711811 (-) 255 WP_003386388.1 hypothetical protein -
  GJ703_RS18180 (GJ703_03484) - 3712202..3712339 (+) 138 WP_012047889.1 hypothetical protein -
  GJ703_RS18190 (GJ703_03485) - 3712773..3712952 (+) 180 WP_003386384.1 aspartyl-phosphate phosphatase Spo0E family protein -
  GJ703_RS18195 (GJ703_03486) sigK 3713378..3714082 (+) 705 WP_012047887.1 RNA polymerase sporulation sigma factor SigK -
  GJ703_RS18200 (GJ703_03487) aroE 3714221..3714997 (+) 777 WP_011986860.1 shikimate dehydrogenase -
  GJ703_RS18205 (GJ703_03488) pilT 3714999..3716051 (+) 1053 WP_003386380.1 type IV pilus twitching motility protein PilT Machinery gene
  GJ703_RS18210 (GJ703_03489) - 3716129..3716272 (-) 144 WP_003386378.1 hypothetical protein -
  GJ703_RS18215 (GJ703_03490) - 3716710..3717984 (+) 1275 WP_003386376.1 glycosyltransferase family 2 protein -
  GJ703_RS18220 (GJ703_03491) ftsA 3718141..3719394 (+) 1254 WP_012047886.1 cell division protein FtsA -
  GJ703_RS18225 (GJ703_03492) ftsZ 3719417..3720526 (+) 1110 WP_003386373.1 cell division protein FtsZ -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39054.11 Da        Isoelectric Point: 8.0876

>NTDB_id=404492 GJ703_RS18205 WP_003386380.1 3714999..3716051(+) (pilT) [Clostridium botulinum strain NCTC 13319]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=404492 GJ703_RS18205 WP_003386380.1 3714999..3716051(+) (pilT) [Clostridium botulinum strain NCTC 13319]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCGGTAGGGGTAGAACC
AACTATAAGAATAAATGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCCTAGGTAGA
TTCAGAGTAAGCGTGTTTAAACAAAGGGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTGAAGATCCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGCAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I4MRH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.286

0.471
  pilT Vibrio cholerae strain A1552

50

94.286

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

99.429

0.46
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilT Neisseria gonorrhoeae MS11

47.463

95.714

0.454
  pilU Acinetobacter baylyi ADP1

41.124

96.571

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.194

95.714

0.394
  pilU Vibrio cholerae strain A1552

38.643

96.857

0.374

Multiple sequence alignment