Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GNG26_RS18455 Genome accession   NZ_CP046446
Coordinates   3899882..3900865 (-) Length   327 a.a.
NCBI ID   WP_156239744.1    Uniprot ID   -
Organism   Leclercia sp. J807     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3894882..3905865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GNG26_RS18425 (GNG26_18425) endA 3895439..3896146 (+) 708 WP_103176920.1 deoxyribonuclease I -
  GNG26_RS18430 (GNG26_18430) rsmE 3896231..3896962 (+) 732 WP_103822814.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GNG26_RS18435 (GNG26_18435) gshB 3896982..3897929 (+) 948 WP_156239743.1 glutathione synthase -
  GNG26_RS18440 (GNG26_18440) - 3898085..3898645 (+) 561 WP_103176923.1 YqgE/AlgH family protein -
  GNG26_RS18445 (GNG26_18445) ruvX 3898645..3899061 (+) 417 WP_103822816.1 Holliday junction resolvase RuvX -
  GNG26_RS18450 (GNG26_18450) - 3899094..3899789 (-) 696 WP_103822817.1 transcriptional regulator -
  GNG26_RS18455 (GNG26_18455) pilT 3899882..3900865 (-) 984 WP_156239744.1 type IV pilus twitching motility protein PilT Machinery gene
  GNG26_RS18460 (GNG26_18460) - 3900883..3901584 (+) 702 WP_156239745.1 YggS family pyridoxal phosphate-dependent enzyme -
  GNG26_RS18465 (GNG26_18465) - 3901606..3902172 (+) 567 WP_103822820.1 YggT family protein -
  GNG26_RS18470 (GNG26_18470) yggU 3902169..3902465 (+) 297 WP_103822821.1 DUF167 family protein YggU -
  GNG26_RS18475 (GNG26_18475) - 3902469..3903062 (+) 594 WP_156239746.1 XTP/dITP diphosphatase -
  GNG26_RS18480 (GNG26_18480) hemW 3903055..3904191 (+) 1137 WP_103822822.1 radical SAM family heme chaperone HemW -
  GNG26_RS18485 (GNG26_18485) - 3904475..3905464 (+) 990 WP_156239747.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 327 a.a.        Molecular weight: 35801.01 Da        Isoelectric Point: 6.8723

>NTDB_id=404351 GNG26_RS18455 WP_156239744.1 3899882..3900865(-) (pilT) [Leclercia sp. J807]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKMEFAPFPGPEVAMLLKSWLSDEQQGAWCAQGQVDFAVTLASKQRLRA
SAFAHMQGCSLALRLLPDECPQLHTLGAPRAIPELLANDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVE
FIHQSKRCLIQQREIGLHCLSFSDALRAALREDPDVILLGELRDSETIRLALTAAETGHVVLATLHTRGAAQAVERLVDA
FPAQEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTYQLPGVIQTGQQAGMQNFEQSLAER
RAQGRLS

Nucleotide


Download         Length: 984 bp        

>NTDB_id=404351 GNG26_RS18455 WP_156239744.1 3899882..3900865(-) (pilT) [Leclercia sp. J807]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCGCCACCTCG
CTGGCGGCGGCGAGGGAAAATGGAATTTGCCCCCTTTCCGGGGCCGGAGGTCGCGATGCTGCTGAAAAGCTGGCTTAGCG
ACGAGCAGCAGGGGGCATGGTGCGCCCAGGGACAGGTGGATTTTGCCGTTACGCTTGCGAGCAAGCAGCGGCTGCGGGCC
AGCGCCTTTGCACATATGCAGGGGTGTTCCCTGGCGCTCCGCCTGCTTCCAGACGAGTGCCCGCAGTTGCATACACTGGG
AGCGCCGCGGGCGATACCTGAACTGCTGGCAAATGATAACGGCCTGATACTGGTGACCGGGGCGACAGGCAGCGGTAAAT
CCACCACGCTTGCGGCGATGGTCGATTTTCTCAATCACCATAGCGACGGACATATCCTGACGCTCGAAGATCCGGTGGAA
TTTATCCATCAGAGCAAGCGTTGCCTGATTCAGCAGCGGGAGATTGGCCTGCATTGCCTCTCCTTTTCTGACGCGCTGCG
GGCAGCATTACGTGAAGATCCCGATGTAATCCTGCTGGGCGAACTGCGTGACAGCGAGACGATCCGCCTTGCGCTTACCG
CCGCCGAGACCGGGCATGTGGTGCTGGCGACGCTTCACACCCGGGGCGCCGCCCAGGCGGTGGAGAGGCTGGTGGATGCC
TTTCCCGCGCAGGAAAAAGAGCCGGTACGTAAACAGCTGGCAGGCAGCCTGCGGGCCGTGCTGGCGCAAAAACTCGAAAG
GGACAACCAGCAGGGGCGCGTCGCGCTGTTCGAGCTGCTGGTTAATACCCCAGCGGCGGCCAATTTGATCCGTGAAGGCA
AAACCTATCAACTGCCCGGCGTGATCCAGACCGGGCAGCAGGCTGGCATGCAAAACTTCGAGCAGAGCCTGGCTGAGCGC
AGGGCGCAGGGGCGATTGTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.492
  pilT Vibrio cholerae strain A1552

49.235

100

0.492
  pilT Neisseria meningitidis 8013

48.476

100

0.486
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.483
  pilT Legionella pneumophila strain ERS1305867

47.826

98.471

0.471
  pilT Legionella pneumophila strain Lp02

47.826

98.471

0.471
  pilT Pseudomonas stutzeri DSM 10701

46.364

100

0.468
  pilT Acinetobacter baumannii strain A118

46.061

100

0.465
  pilT Acinetobacter baumannii D1279779

46.061

100

0.465
  pilT Acinetobacter nosocomialis M2

45.758

100

0.462
  pilT Pseudomonas aeruginosa PAK

45.758

100

0.462
  pilT Acinetobacter baylyi ADP1

46.177

100

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.471

0.428
  pilU Vibrio cholerae strain A1552

38.788

100

0.391
  pilU Pseudomonas stutzeri DSM 10701

37.462

100

0.379
  pilU Acinetobacter baylyi ADP1

37.538

99.388

0.373

Multiple sequence alignment