Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GMW39_RS24065 Genome accession   NZ_CP046376
Coordinates   5156714..5157730 (+) Length   338 a.a.
NCBI ID   WP_025919325.1    Uniprot ID   -
Organism   Pectobacterium parmentieri strain HC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5151714..5162730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GMW39_RS24035 (GMW39_24110) hemW 5152432..5153571 (-) 1140 WP_014701260.1 radical SAM family heme chaperone HemW -
  GMW39_RS24040 (GMW39_24115) - 5153564..5154157 (-) 594 WP_014701259.1 XTP/dITP diphosphatase -
  GMW39_RS24045 (GMW39_24120) yggU 5154175..5154465 (-) 291 WP_014701258.1 DUF167 family protein YggU -
  GMW39_RS24050 (GMW39_24125) - 5154462..5155031 (-) 570 WP_014701257.1 YggT family protein -
  GMW39_RS24055 (GMW39_24130) proC 5155081..5155902 (-) 822 WP_015731141.1 pyrroline-5-carboxylate reductase -
  GMW39_RS24060 (GMW39_24135) - 5155979..5156692 (-) 714 WP_014701255.1 YggS family pyridoxal phosphate-dependent enzyme -
  GMW39_RS24065 (GMW39_24140) pilT 5156714..5157730 (+) 1017 WP_025919325.1 type IV pilus twitching motility protein PilT Machinery gene
  GMW39_RS24070 (GMW39_24145) - 5157699..5159531 (-) 1833 WP_014701253.1 diguanylate cyclase -
  GMW39_RS24075 (GMW39_24150) - 5160036..5161607 (-) 1572 WP_014701252.1 methyl-accepting chemotaxis protein -
  GMW39_RS24080 (GMW39_24155) nsrR 5161896..5162321 (+) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36918.28 Da        Isoelectric Point: 6.3631

>NTDB_id=403606 GMW39_RS24065 WP_025919325.1 5156714..5157730(+) (pilT) [Pectobacterium parmentieri strain HC]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPVQREQLRQIGQVDGTLMLPDGQ
RLRVNVFHQREGLSVALRIIPFFQPSLDELHAPPVLATLLEKPNGLILITGATGSGKSTTLAAMVGALNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSAPQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVAQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLMQTGVAAGMQTFEQS
DQQRCRDGLISHSCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=403606 GMW39_RS24065 WP_025919325.1 5156714..5157730(+) (pilT) [Pectobacterium parmentieri strain HC]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCACCCTCCGGT
GTTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAACAGGTGGCACAATGGTGTGATG
CCTGGCTCGAACCGGTTCAGAGAGAGCAGTTGCGGCAGATTGGGCAAGTGGACGGCACGTTAATGTTGCCTGATGGGCAA
CGGCTGCGGGTCAATGTGTTTCACCAGCGGGAAGGGCTGTCTGTCGCGCTGCGTATTATCCCGTTTTTTCAACCTTCATT
GGATGAATTACATGCGCCACCCGTTCTTGCGACGCTGTTGGAGAAACCAAATGGGCTGATCCTGATTACTGGTGCGACGG
GCAGCGGTAAATCTACGACGTTGGCGGCAATGGTCGGGGCGTTAAATGACAGCAGCGATCGCCATGTGATCACGTTGGAA
GATCCGATCGAATTTATTCATACCAGCCGACGCTGCCTGATCCAACAACGAGAGATCGGTGCACACAGCGCGTCTTTTGC
GCAGGCGCTACGGGCGGCATTAAGGGAAGATCCCGATGTTATTCTACTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGTTAACGGCGGCAGAAACCGGTCATCTGGTGCTGTCAACGTTACATACACGTAGTGCACCGCAGGCTGTTGATCGG
TTGATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCCGTGGTGGCGCA
AAAATTGTTGCCCGCAGCACAAGGTGGGCGAATCGCGCTGTATGAAGTGCTGACTGCCACCGCGGCAGTCAGCAACTTGA
TTCGTGAGGGGAAAACGCACCAGCTTCCCGGCCTGATGCAAACCGGAGTTGCTGCGGGAATGCAAACGTTCGAACAAAGC
GACCAGCAGCGTTGCCGGGATGGGCTTATTTCACACAGTTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50

99.408

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

97.633

0.497
  pilT Vibrio cholerae strain A1552

50.909

97.633

0.497
  pilT Neisseria gonorrhoeae MS11

49.702

99.408

0.494
  pilT Legionella pneumophila strain Lp02

48.06

99.112

0.476
  pilT Legionella pneumophila strain ERS1305867

48.06

99.112

0.476
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Pseudomonas aeruginosa PAK

47.761

99.112

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.761

99.112

0.473
  pilT Acinetobacter nosocomialis M2

46.866

99.112

0.465
  pilT Acinetobacter baumannii D1279779

46.866

99.112

0.465
  pilT Acinetobacter baumannii strain A118

46.866

99.112

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373

Multiple sequence alignment