Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SAR11G3_RS01075 Genome accession   NC_015380
Coordinates   210355..211368 (-) Length   337 a.a.
NCBI ID   WP_013694877.1    Uniprot ID   F2I3R2
Organism   Candidatus Pelagibacter sp. IMCC9063     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 205355..216368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAR11G3_RS01050 (SAR11G3_00231) - 206911..207450 (-) 540 WP_013694871.1 hypothetical protein -
  SAR11G3_RS01055 (SAR11G3_00232) - 207454..208032 (-) 579 WP_013694872.1 hypothetical protein -
  SAR11G3_RS07440 (SAR11G3_00233) - 208044..208232 (-) 189 WP_013694873.1 PilX N-terminal domain-containing pilus assembly protein -
  SAR11G3_RS01060 (SAR11G3_00234) - 208241..209128 (-) 888 WP_013694874.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SAR11G3_RS01065 (SAR11G3_00235) - 209128..209694 (-) 567 WP_148225710.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SAR11G3_RS01070 (SAR11G3_00236) - 209700..210392 (-) 693 WP_013694876.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SAR11G3_RS01075 (SAR11G3_00237) pilT 210355..211368 (-) 1014 WP_013694877.1 type IV pilus twitching motility protein PilT Machinery gene
  SAR11G3_RS01080 (SAR11G3_00238) - 211426..213867 (+) 2442 WP_148225690.1 autotransporter outer membrane beta-barrel domain-containing protein -
  SAR11G3_RS01085 (SAR11G3_00239) - 213867..214211 (+) 345 WP_013694879.1 hypothetical protein -
  SAR11G3_RS01090 (SAR11G3_00240) - 214220..216025 (-) 1806 WP_158304707.1 hypothetical protein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37196.81 Da        Isoelectric Point: 7.2236

>NTDB_id=40291 SAR11G3_RS01075 WP_013694877.1 210355..211368(-) (pilT) [Candidatus Pelagibacter sp. IMCC9063]
MFEKLEELAKNNKKISDFHIRSGSPLAYRQTGEVVTVKDILVKAQDLQDLMSTNCNETETKKFQETHELDSAVMLSGLRF
RANFYKTIRGPAAVLRRVESVIPEMSQFDLPDVLYDIIDMHKGLVLVTGPTGSGKSTTLAAIINEINKTRTAHIITVEDP
VEFVHKDAKSIVSQREVGKQTQSFASALKAALREDPDVILVGEMRDLETVGLALTAAETGHLVFGTLHTSGAPNTINRII
DVFPPEQQAQIRAQISTSLKMVVTQRLIKTKDGQGRVAAFEVMKCTAPIQNLIREAKIHQIPSIMQTSVRDGMITMQKSL
DELVNNGKIDPGAGKEH

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=40291 SAR11G3_RS01075 WP_013694877.1 210355..211368(-) (pilT) [Candidatus Pelagibacter sp. IMCC9063]
ATGTTTGAAAAATTAGAAGAATTAGCAAAAAATAATAAAAAAATTTCCGATTTTCATATTCGAAGTGGCTCACCGTTAGC
TTATAGACAAACAGGTGAAGTAGTGACGGTTAAAGATATTCTTGTAAAAGCACAAGATCTTCAAGATCTTATGTCGACAA
ATTGCAATGAAACAGAAACAAAAAAATTTCAAGAAACGCATGAATTAGATTCTGCTGTAATGTTAAGCGGATTAAGGTTT
AGAGCTAACTTTTATAAAACAATTAGAGGGCCTGCTGCAGTTCTTAGAAGAGTGGAAAGTGTCATTCCTGAAATGTCACA
ATTTGATTTACCAGATGTTCTTTATGATATTATTGATATGCATAAAGGGTTAGTTCTAGTTACAGGACCAACAGGGTCTG
GAAAATCAACTACACTTGCAGCAATTATTAATGAAATTAACAAAACAAGAACAGCGCATATTATTACAGTTGAAGACCCG
GTGGAGTTTGTGCACAAAGATGCAAAAAGTATTGTTTCACAGCGTGAAGTTGGAAAACAAACACAATCATTTGCAAGTGC
TTTAAAGGCAGCTTTGCGTGAAGATCCAGATGTTATTTTAGTGGGAGAGATGAGAGATCTTGAAACAGTTGGTCTTGCCT
TAACTGCTGCAGAAACAGGTCATTTAGTTTTTGGAACATTGCATACTAGTGGAGCGCCCAATACTATTAATAGAATTATT
GATGTATTTCCTCCAGAACAACAAGCACAAATTCGTGCGCAAATTTCCACTTCATTAAAAATGGTAGTAACTCAAAGATT
AATAAAAACAAAAGATGGTCAAGGCCGAGTGGCTGCTTTTGAAGTTATGAAATGTACAGCTCCAATACAAAACTTAATAC
GTGAAGCTAAAATTCACCAAATCCCAAGTATCATGCAAACGTCAGTTCGTGATGGTATGATTACAATGCAAAAATCATTA
GATGAACTTGTTAATAATGGGAAAATTGATCCAGGTGCAGGAAAAGAACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2I3R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

50

97.329

0.487
  pilT Legionella pneumophila strain Lp02

50.617

96.142

0.487
  pilT Legionella pneumophila strain ERS1305867

50.617

96.142

0.487
  pilT Neisseria meningitidis 8013

50

96.142

0.481
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.632

97.626

0.475
  pilT Vibrio cholerae strain A1552

48.632

97.626

0.475
  pilT Acinetobacter baumannii strain A118

49.211

94.065

0.463
  pilT Acinetobacter nosocomialis M2

49.211

94.065

0.463
  pilT Acinetobacter baumannii D1279779

49.211

94.065

0.463
  pilT Pseudomonas stutzeri DSM 10701

48.137

95.549

0.46
  pilT Acinetobacter baylyi ADP1

48.276

94.659

0.457
  pilT Pseudomonas aeruginosa PAK

48.889

93.472

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.923

92.878

0.445
  pilU Pseudomonas stutzeri DSM 10701

40.426

97.626

0.395
  pilU Vibrio cholerae strain A1552

42.089

93.769

0.395
  pilU Acinetobacter baylyi ADP1

38.344

96.736

0.371

Multiple sequence alignment