Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSEBR_RS28480 Genome accession   NC_015379
Coordinates   6442875..6443909 (-) Length   344 a.a.
NCBI ID   WP_003206482.1    Uniprot ID   A0AAD1LYK7
Organism   Pseudomonas brassicacearum subsp. brassicacearum NFM421     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6437875..6448909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSEBR_RS28450 (PSEBR_a5314) ruvX 6438008..6438445 (+) 438 WP_003177497.1 Holliday junction resolvase RuvX -
  PSEBR_RS28455 (PSEBR_a5315) pyrR 6438530..6439036 (+) 507 WP_003206474.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PSEBR_RS28460 (PSEBR_a5316) - 6439061..6440065 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  PSEBR_RS28465 (PSEBR_a5317) - 6440062..6441333 (+) 1272 WP_013694464.1 dihydroorotase -
  PSEBR_RS28470 (PSEBR_a5318) - 6441457..6441885 (-) 429 WP_003206477.1 NINE protein -
  PSEBR_RS28475 (PSEBR_a5319) - 6442135..6442746 (+) 612 WP_003206478.1 C40 family peptidase -
  PSEBR_RS28480 (PSEBR_a5320) pilT 6442875..6443909 (-) 1035 WP_003206482.1 type IV pilus twitching motility protein PilT Machinery gene
  PSEBR_RS28485 (PSEBR_a5321) - 6443963..6444649 (+) 687 WP_013694465.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSEBR_RS28490 (PSEBR_a5322) proC 6444685..6445503 (+) 819 WP_013694466.1 pyrroline-5-carboxylate reductase -
  PSEBR_RS28495 (PSEBR_a5323) - 6445514..6446104 (+) 591 WP_003206487.1 YggT family protein -
  PSEBR_RS28500 (PSEBR_a5324) - 6446110..6446403 (+) 294 WP_003206488.1 DUF167 domain-containing protein -
  PSEBR_RS28505 (PSEBR_a5325) metX 6446707..6447846 (+) 1140 WP_003206490.1 homoserine O-succinyltransferase MetX -
  PSEBR_RS28510 (PSEBR_a5326) metW 6447854..6448474 (+) 621 WP_003206492.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38162.80 Da        Isoelectric Point: 6.9778

>NTDB_id=40285 PSEBR_RS28480 WP_003206482.1 6442875..6443909(-) (pilT) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVNKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=40285 PSEBR_RS28480 WP_003206482.1 6442875..6443909(-) (pilT) [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGGATATCACTGAGTTACTGGCGTTCAGCGCCAAGCAGGGCGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCACCGAT
GATCCGCGTCGATGGCGATGTCAGGCGCATCAACCTGCCGGCACTGGACCACAAGCAGGTTCATGAACTGATCTACGACA
TCATGAACGACCGGCAGCGAGTAGATTACGAAAAGTTTCTGGAGACTGACTTTTCTTTCGAGGTACCGGGCGTCGCGCGG
TTTCGGGTCAACGCTTTCAACCAGAATCGCGGTGCGGGGGCGGTGTTTCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTTCGCAAAGTGACCGAGGCGCCTCGTGGGCTGGTGTTGGTGACCGGGCCGACCGGGT
CAGGCAAATCCACCACCCTGGCGGCCATGATCGATTACTTGAACAGCCACAAGCACCATCACATCCTCACCATCGAAGAC
CCTATCGAATTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGTGAGGTGCACCGCGACACCCAGGGTTTCTCCAC
GGCCCTGCGTTCGGCCCTGCGGGAGGACCCGGACGTGATCCTGGTGGGGGAGATGCGCGACCTTGAAACCATTCGCCTGG
CGCTGACCGCCGCGGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCTGGTGACGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAATCGTTGCAGGCGGTGATTTCCCAGAC
GCTGGTCAAGAAAATCGGCGGCGGCCGGATCGCGGCGCATGAAATCATGCTGGGTACCTCGGCCATCCGGAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGTTCGCTGGGGATGCAGACGCTGGACATGTGCCTG
AAGGACTTGGTGAACAAGGGGCTGATCAGCCGCGAACATGCGCGGGAGCGGGCGCGTACGCCGGATAACTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.953

100

0.89
  pilT Pseudomonas stutzeri DSM 10701

88.663

100

0.887
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

78.488

100

0.785
  pilT Legionella pneumophila strain Lp02

72.781

98.256

0.715
  pilT Legionella pneumophila strain ERS1305867

72.781

98.256

0.715
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

65.797

100

0.66
  pilT Neisseria gonorrhoeae MS11

65.507

100

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398

Multiple sequence alignment