Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HIPMA_RS02455 Genome accession   NC_015318
Coordinates   478669..479763 (-) Length   364 a.a.
NCBI ID   WP_013681488.1    Uniprot ID   F2LUC1
Organism   Hippea maritima DSM 10411     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473669..484763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HIPMA_RS02435 (Hipma_0471) - 474453..475226 (+) 774 WP_013681484.1 rod shape-determining protein MreC -
  HIPMA_RS02440 (Hipma_0472) - 475208..475687 (+) 480 WP_013681485.1 hypothetical protein -
  HIPMA_RS02445 (Hipma_0473) mrdA 475671..477467 (+) 1797 WP_013681486.1 penicillin-binding protein 2 -
  HIPMA_RS02450 (Hipma_0474) - 477460..478665 (-) 1206 WP_013681487.1 type II secretion system F family protein -
  HIPMA_RS02455 (Hipma_0475) pilT 478669..479763 (-) 1095 WP_013681488.1 type IV pilus twitching motility protein PilT Machinery gene
  HIPMA_RS02460 (Hipma_0476) pilB 479786..481483 (-) 1698 WP_013681489.1 type IV-A pilus assembly ATPase PilB -
  HIPMA_RS02465 (Hipma_0477) aroE 481480..482301 (-) 822 WP_013681490.1 shikimate dehydrogenase -
  HIPMA_RS02470 (Hipma_0478) tsaE 482285..482707 (-) 423 WP_013681491.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  HIPMA_RS02475 (Hipma_0479) - 482768..483748 (+) 981 WP_013681492.1 glycosyltransferase family 9 protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40631.99 Da        Isoelectric Point: 6.8543

>NTDB_id=40245 HIPMA_RS02455 WP_013681488.1 478669..479763(-) (pilT) [Hippea maritima DSM 10411]
MGKKAQDLVELLEKMISIQASDLHITAGARPKVRVDSELMDMEEYDELSPADAQNLCYSVMTELQKKTLEEDFDVDFSFG
IPKLSRFRANVYIQRGTIAGAFRMIPFEIPQFDKLGIPPVVQQFAHLKKGIVLVTGPTGSGKSTTLAALLDKINAERKVH
IITIEDPIEFTYSHKLALIDQREVGSDVKSFASALKHILRQDPDVILIGEMRDLETIQAALTVAETGHLVFATLHTNSAP
ETINRIIDVFPPNQQEQVRTQLSVALQGVASQTLIKRKDGKGRVLAMEIMIPNSAIRNLIRENKIPQIYSSMQMGQTETG
MMTMNQSIISLYKKGFISYEAAIEASNDKKAIQRELDKIRYAKG

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=40245 HIPMA_RS02455 WP_013681488.1 478669..479763(-) (pilT) [Hippea maritima DSM 10411]
ATGGGTAAAAAGGCTCAAGATTTAGTAGAGCTACTGGAAAAGATGATAAGCATTCAGGCATCGGATTTACATATAACAGC
CGGCGCAAGACCAAAGGTAAGAGTGGACAGTGAACTTATGGACATGGAAGAATACGACGAGCTCTCCCCAGCCGATGCCC
AAAACCTCTGCTACAGCGTAATGACAGAACTACAGAAAAAAACATTAGAGGAAGATTTTGATGTTGACTTTTCATTCGGA
ATCCCAAAACTATCCCGCTTCAGGGCAAATGTTTATATACAAAGGGGTACAATAGCAGGCGCCTTTAGAATGATACCATT
TGAGATACCCCAGTTTGATAAATTAGGCATACCACCTGTAGTACAGCAATTTGCCCACTTAAAAAAAGGAATAGTATTGG
TTACAGGACCAACAGGAAGTGGAAAATCAACTACACTTGCTGCGTTGTTGGATAAAATTAACGCAGAAAGAAAGGTGCAT
ATAATTACAATAGAGGATCCAATAGAGTTCACATATTCGCACAAATTAGCCCTTATAGACCAAAGGGAGGTTGGAAGTGA
TGTAAAGAGCTTTGCCTCAGCCCTAAAGCATATACTTCGCCAAGACCCTGATGTAATCCTTATTGGTGAGATGAGAGACC
TTGAAACAATACAGGCAGCTTTAACCGTTGCCGAAACAGGACATTTGGTCTTTGCAACACTTCACACAAATTCAGCTCCA
GAAACAATAAACAGAATAATAGATGTATTCCCACCCAATCAGCAAGAACAGGTCAGAACGCAGCTATCTGTTGCACTCCA
AGGGGTTGCATCCCAAACCCTCATCAAAAGAAAAGATGGCAAAGGCAGGGTATTGGCCATGGAAATAATGATACCAAACT
CAGCCATTAGAAACCTCATAAGAGAAAACAAGATACCTCAAATTTACTCATCGATGCAAATGGGCCAAACAGAAACAGGG
ATGATGACCATGAATCAATCAATAATTAGTCTTTATAAAAAAGGATTCATAAGCTACGAAGCAGCTATCGAGGCAAGCAA
TGATAAAAAGGCAATTCAAAGGGAGCTTGATAAAATAAGATACGCAAAGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2LUC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.187

94.231

0.492
  pilT Legionella pneumophila strain ERS1305867

52.187

94.231

0.492
  pilT Acinetobacter baylyi ADP1

52.083

92.308

0.481
  pilT Acinetobacter nosocomialis M2

50.882

93.407

0.475
  pilT Acinetobacter baumannii D1279779

50.882

93.407

0.475
  pilT Acinetobacter baumannii strain A118

50.882

93.407

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.425

95.604

0.473
  pilT Pseudomonas aeruginosa PAK

50

92.857

0.464
  pilT Neisseria meningitidis 8013

51.385

89.286

0.459
  pilT Pseudomonas stutzeri DSM 10701

49.112

92.857

0.456
  pilT Neisseria gonorrhoeae MS11

50.926

89.011

0.453
  pilT Vibrio cholerae strain A1552

49.849

90.934

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.849

90.934

0.453
  pilU Acinetobacter baylyi ADP1

40.936

93.956

0.385
  pilU Vibrio cholerae strain A1552

40.762

93.681

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.766

93.956

0.374

Multiple sequence alignment