Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GFS31_RS09970 Genome accession   NZ_CP046155
Coordinates   2241734..2242837 (+) Length   367 a.a.
NCBI ID   WP_198804700.1    Uniprot ID   A0A7T5E3P0
Organism   Leptolyngbya sp. BL0902     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2236734..2247837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFS31_RS09960 (GFS31_21010) grpE 2238246..2238920 (-) 675 WP_198804698.1 nucleotide exchange factor GrpE -
  GFS31_RS09965 (GFS31_21020) pilB/pilB1 2239520..2241523 (+) 2004 WP_198804699.1 GspE/PulE family protein Machinery gene
  GFS31_RS09970 (GFS31_21030) pilT 2241734..2242837 (+) 1104 WP_198804700.1 type IV pilus twitching motility protein PilT Machinery gene
  GFS31_RS09975 (GFS31_21040) - 2242930..2244150 (+) 1221 WP_198804701.1 type II secretion system F family protein -
  GFS31_RS09980 (GFS31_21050) - 2244308..2244721 (-) 414 WP_317135019.1 metalloregulator ArsR/SmtB family transcription factor -
  GFS31_RS09985 (GFS31_21060) - 2244856..2245347 (-) 492 WP_198804702.1 hypothetical protein -
  GFS31_RS09990 (GFS31_21070) - 2245500..2246060 (-) 561 WP_225907365.1 cofactor assembly of complex C subunit B -
  GFS31_RS09995 (GFS31_21080) - 2246212..2246601 (-) 390 WP_198804703.1 PadR family transcriptional regulator -
  GFS31_RS10000 (GFS31_21100) - 2246914..2247090 (-) 177 WP_198804704.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40029.21 Da        Isoelectric Point: 6.3975

>NTDB_id=401707 GFS31_RS09970 WP_198804700.1 2241734..2242837(+) (pilT) [Leptolyngbya sp. BL0902]
MELMIEDLMEEVIERGGSDLHLSAGLPPYIRISGKLTPTEHEPMTSEACQRLIFSMLNNTQRKQLEQTWELDCSYGVKGL
ARFRVNVYKDRGTYAACLRALSSKIPSMEVLGLPPIVHELAEKPRGLILVTGPTGSGKSTTLASMINNINLTRPEHILTV
EDPIEFVYEPIKSLIHQRQIGEDTKSFANALRAALREDPDVILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RMIDVFPPEQQQQVRVQLSGSLVAVLSQTLVPKANVKPGEYGRVMAQEIMVITPAIANMIREGKTAQIYGAIQTGGKVGM
QTLEKVLADLYKSGTISFEAAMSKTSKPDELQRLLGPAPAAAGAGRR

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=401707 GFS31_RS09970 WP_198804700.1 2241734..2242837(+) (pilT) [Leptolyngbya sp. BL0902]
ATGGAATTGATGATCGAAGATTTGATGGAAGAGGTAATCGAGCGGGGCGGCTCTGACCTCCACCTCTCGGCGGGACTGCC
CCCCTACATTCGGATCAGCGGCAAACTAACGCCCACCGAGCACGAGCCCATGACCTCGGAAGCCTGTCAGCGGCTGATCT
TCAGCATGTTGAACAACACCCAGCGCAAGCAGCTAGAGCAAACCTGGGAACTGGACTGTTCCTACGGGGTGAAGGGGCTG
GCCCGCTTCCGGGTCAACGTATACAAAGACCGAGGCACCTACGCCGCCTGTCTGCGGGCGCTCAGCTCCAAAATTCCCAG
CATGGAAGTTCTGGGATTACCTCCCATCGTCCACGAACTGGCCGAAAAACCGCGCGGCCTCATTCTGGTGACGGGGCCAA
CGGGTTCCGGGAAATCCACCACCCTGGCCTCGATGATCAACAACATCAACCTCACCCGTCCAGAGCACATTCTGACGGTG
GAAGACCCCATCGAGTTTGTCTATGAGCCAATTAAGAGCCTGATTCACCAGCGCCAAATTGGGGAAGATACCAAGAGCTT
TGCCAACGCCCTGCGGGCCGCCCTGCGGGAAGACCCCGACGTGATTCTGGTGGGGGAAATGCGTGACCTAGAAACCATCT
CCCTGGCCATTTCCGCCGCAGAAACGGGCCACTTAGTCTTTGGCACCCTGCACACCAGTTCCGCCGCCCAAACCGTAGAC
CGGATGATTGACGTGTTCCCCCCAGAGCAGCAGCAGCAGGTGCGGGTGCAGCTTTCCGGCTCCCTAGTGGCGGTGCTCAG
CCAAACCCTAGTGCCCAAGGCCAACGTCAAACCGGGCGAGTATGGTCGGGTGATGGCCCAGGAAATCATGGTGATTACCC
CCGCAATTGCCAACATGATTCGGGAGGGCAAAACCGCCCAAATCTACGGGGCCATCCAAACCGGGGGCAAAGTGGGGATG
CAGACCCTTGAGAAGGTACTAGCAGACCTTTACAAGTCTGGCACTATCTCCTTTGAAGCCGCCATGTCTAAGACCTCTAA
GCCCGACGAACTCCAGCGCCTCCTTGGCCCAGCACCTGCCGCTGCCGGAGCTGGGCGTCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T5E3P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.314

94.55

0.504
  pilT Legionella pneumophila strain ERS1305867

53.333

94.005

0.501
  pilT Legionella pneumophila strain Lp02

53.333

94.005

0.501
  pilT Acinetobacter baylyi ADP1

52.738

94.55

0.499
  pilT Pseudomonas stutzeri DSM 10701

52.738

94.55

0.499
  pilT Acinetobacter nosocomialis M2

52.161

94.55

0.493
  pilT Acinetobacter baumannii strain A118

52.161

94.55

0.493
  pilT Acinetobacter baumannii D1279779

52.161

94.55

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.611

98.093

0.477
  pilT Neisseria gonorrhoeae MS11

50.143

95.095

0.477
  pilT Neisseria meningitidis 8013

50.143

95.095

0.477
  pilT Vibrio cholerae strain A1552

51.176

92.643

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.176

92.643

0.474
  pilU Acinetobacter baylyi ADP1

39.031

95.64

0.373
  pilU Pseudomonas stutzeri DSM 10701

38.177

95.64

0.365

Multiple sequence alignment