Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   GFS31_RS09965 Genome accession   NZ_CP046155
Coordinates   2239520..2241523 (+) Length   667 a.a.
NCBI ID   WP_198804699.1    Uniprot ID   A0A7T5JF17
Organism   Leptolyngbya sp. BL0902     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2234520..2246523
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFS31_RS09945 (GFS31_20980) - 2234607..2234864 (-) 258 WP_198804695.1 sulfurtransferase TusA family protein -
  GFS31_RS09950 (GFS31_20990) dnaJ 2234870..2235997 (-) 1128 WP_198804696.1 molecular chaperone DnaJ -
  GFS31_RS09955 (GFS31_21000) dnaK 2236116..2238068 (-) 1953 WP_198804697.1 molecular chaperone DnaK -
  GFS31_RS09960 (GFS31_21010) grpE 2238246..2238920 (-) 675 WP_198804698.1 nucleotide exchange factor GrpE -
  GFS31_RS09965 (GFS31_21020) pilB/pilB1 2239520..2241523 (+) 2004 WP_198804699.1 GspE/PulE family protein Machinery gene
  GFS31_RS09970 (GFS31_21030) pilT 2241734..2242837 (+) 1104 WP_198804700.1 type IV pilus twitching motility protein PilT Machinery gene
  GFS31_RS09975 (GFS31_21040) - 2242930..2244150 (+) 1221 WP_198804701.1 type II secretion system F family protein -
  GFS31_RS09980 (GFS31_21050) - 2244308..2244721 (-) 414 WP_317135019.1 metalloregulator ArsR/SmtB family transcription factor -
  GFS31_RS09985 (GFS31_21060) - 2244856..2245347 (-) 492 WP_198804702.1 hypothetical protein -
  GFS31_RS09990 (GFS31_21070) - 2245500..2246060 (-) 561 WP_225907365.1 cofactor assembly of complex C subunit B -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73693.40 Da        Isoelectric Point: 4.8358

>NTDB_id=401706 GFS31_RS09965 WP_198804699.1 2239520..2241523(+) (pilB/pilB1) [Leptolyngbya sp. BL0902]
MTNSPSSRRALVLQRSFSPFGNKLIQTGYVDSEQMQKALAESRKTGQSLTDVLESLTGQQLAPELLRQYKKQQLFELKIL
YGVESLDPELSDVSAAQIGSLIDTLIPVDVCRRHRLVPLAQEEGDQPAVLVAMVDPENLEAQDDLNRILRPRNMALRRMV
ITVEDFQRLISTYLDEAVAKQKKEELDAAVDVRTSLEELDYGGDLEDVIDQEADLGEALKDAGAAPVIALVNKILVKALQ
EGVSDIHIEPQEEYLRVRFRKDGVLKEALPRMPKKIIPAVTARFKIISDLDIAERRAPQDGRIRRIFQGRKVDFRVNTLP
SRCGEKVVLRILDNSVTQLGLDKLITDPTSLKIVQEMAARPFGLLLVTGPTGSGKTTTLYSVLSERNDPGINISTAEDPI
EYTLEGLTQVQVIREKGMDFSAILRAFLRQDPDVILVGETRDRETAKTAIEAALTGHMVLTTLHTNDAAGAIARLDEMGV
ESFMVSSALIGVLAQRLLRRVCGDCRIAYQPSVDELAHFGLSASADGEVTFYKANTLSIDQVAEARAKNTLCPTCQGGGY
KGRVGVYEVLRVTERIQKLISDGAPTEVIKEAAVEEGMQTLLAYSLSLVRQGYTTLDEVERVTFTDTGLEAELKAKRKAS
LTCSCCSAELQQEWLDCPYCLTPRFAE

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=401706 GFS31_RS09965 WP_198804699.1 2239520..2241523(+) (pilB/pilB1) [Leptolyngbya sp. BL0902]
ATGACTAATTCTCCTTCGTCTCGGCGGGCGCTTGTTCTACAACGAAGTTTTTCGCCCTTTGGCAATAAGCTGATCCAAAC
GGGCTACGTAGATTCGGAACAGATGCAGAAAGCCCTCGCGGAGAGCCGCAAAACCGGGCAATCCCTGACCGATGTCCTCG
AGTCTTTGACCGGGCAGCAGTTGGCCCCAGAACTGCTGCGCCAGTACAAGAAGCAGCAACTCTTTGAGCTGAAGATTCTC
TACGGGGTCGAGTCCCTCGATCCAGAACTCAGCGATGTCTCAGCCGCCCAGATCGGTAGCCTGATTGACACGCTCATTCC
GGTGGATGTCTGCCGCCGCCACCGCCTCGTCCCCCTAGCCCAAGAGGAGGGGGATCAGCCAGCGGTACTCGTGGCCATGG
TCGATCCTGAAAACCTTGAGGCCCAGGACGACCTGAACCGCATCCTGCGCCCCCGCAACATGGCCCTGCGCCGCATGGTG
ATTACCGTTGAAGACTTTCAGCGGCTCATCTCCACCTACCTCGATGAGGCGGTTGCCAAGCAGAAAAAAGAAGAACTCGA
TGCCGCCGTTGACGTCCGCACCAGCCTAGAGGAACTGGATTATGGTGGCGACCTAGAGGACGTCATTGACCAGGAAGCTG
ATCTTGGCGAGGCCCTGAAGGATGCTGGGGCGGCTCCGGTCATTGCCCTAGTGAACAAAATTCTGGTGAAGGCGCTGCAA
GAGGGCGTTTCCGACATCCACATCGAGCCCCAGGAAGAGTATCTGCGGGTGCGCTTCCGCAAAGATGGGGTGCTGAAGGA
AGCCCTGCCCCGGATGCCGAAAAAAATCATCCCAGCGGTAACAGCCCGCTTCAAGATCATCTCCGACCTCGACATTGCCG
AGCGACGGGCTCCCCAGGATGGCCGGATTCGCCGGATCTTCCAGGGTCGTAAGGTAGACTTTCGGGTCAACACCTTGCCC
AGCCGCTGCGGCGAGAAGGTGGTGCTGCGGATTCTGGATAACTCCGTCACCCAACTGGGGCTCGACAAGCTCATCACCGA
CCCCACCTCCCTCAAAATTGTCCAGGAAATGGCAGCGCGGCCCTTTGGGCTCTTGCTAGTGACGGGGCCAACGGGTTCCG
GGAAAACCACGACCCTCTACTCGGTGCTGTCGGAGCGCAATGATCCTGGCATCAACATCAGCACCGCCGAAGACCCGATT
GAATACACCCTAGAAGGGCTCACCCAGGTGCAGGTGATTCGGGAAAAGGGCATGGACTTTTCCGCCATTCTGCGGGCCTT
TTTGCGCCAAGACCCGGATGTGATTCTGGTGGGGGAAACCCGCGACCGAGAAACCGCCAAAACCGCCATTGAAGCGGCCC
TCACCGGGCACATGGTGCTCACCACCCTACACACCAACGACGCCGCCGGGGCCATCGCCCGTTTGGATGAAATGGGGGTG
GAATCCTTCATGGTGTCTAGCGCCCTGATTGGGGTGCTGGCCCAACGCCTCCTGCGGCGGGTCTGCGGCGATTGCCGCAT
TGCCTACCAGCCCAGCGTGGACGAACTGGCCCACTTTGGCCTCAGCGCCTCCGCCGATGGCGAAGTGACCTTCTACAAGG
CCAACACCCTCTCCATCGACCAGGTGGCCGAAGCGCGGGCAAAAAATACCCTCTGTCCCACCTGTCAGGGCGGTGGCTAT
AAAGGCCGGGTCGGGGTCTATGAAGTGCTGCGGGTGACGGAGCGCATTCAAAAGCTGATCTCCGACGGGGCACCGACGGA
GGTGATCAAAGAAGCCGCCGTAGAGGAGGGAATGCAGACCCTGCTGGCCTACAGTCTGTCCCTGGTGCGCCAGGGCTATA
CCACCCTCGATGAAGTCGAGCGCGTTACCTTTACCGACACGGGCCTAGAGGCCGAACTGAAGGCAAAGCGGAAAGCGTCG
CTGACCTGTAGCTGTTGTAGCGCCGAATTGCAGCAGGAGTGGTTAGACTGCCCCTACTGCCTCACCCCCCGCTTTGCGGA
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(233-500)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T5JF17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.066

100

0.672

Multiple sequence alignment