Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CLOLE_RS08740 Genome accession   NC_015275
Coordinates   1939078..1940142 (+) Length   354 a.a.
NCBI ID   WP_013656738.1    Uniprot ID   F2JMC3
Organism   Cellulosilyticum lentocellum DSM 5427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1934078..1945142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLOLE_RS08730 (Clole_1715) - 1934549..1937176 (+) 2628 WP_013656736.1 YfhO family protein -
  CLOLE_RS08735 (Clole_1716) - 1937375..1939057 (+) 1683 WP_013656737.1 GspE/PulE family protein -
  CLOLE_RS08740 (Clole_1717) pilT 1939078..1940142 (+) 1065 WP_013656738.1 type IV pilus twitching motility protein PilT Machinery gene
  CLOLE_RS08745 (Clole_1718) - 1940155..1941369 (+) 1215 WP_013656739.1 type II secretion system F family protein -
  CLOLE_RS08750 (Clole_1719) - 1941427..1941843 (+) 417 WP_013656740.1 type II secretion system protein -
  CLOLE_RS08755 (Clole_1720) - 1941930..1942598 (+) 669 WP_013656741.1 A24 family peptidase -
  CLOLE_RS08760 (Clole_1721) - 1942616..1943143 (+) 528 WP_013656742.1 Tfp pilus assembly protein FimT/FimU -
  CLOLE_RS08765 (Clole_1722) - 1943161..1943730 (+) 570 WP_013656743.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CLOLE_RS08770 (Clole_1723) pilM 1943799..1944806 (+) 1008 WP_013656744.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39623.61 Da        Isoelectric Point: 6.7271

>NTDB_id=40115 CLOLE_RS08740 WP_013656738.1 1939078..1940142(+) (pilT) [Cellulosilyticum lentocellum DSM 5427]
MDVNDLLEIVVEQNASDIHLTVGLPPTLRLHGQLTPLDLPTLREADTKELIGQLLEGKPILMEKFREKGEVDFSYALQDI
GRFRVNVYKQRGSWATALRLVGVDIPRMEDLGLPRKILESLSDNTRGLILVTGPTGSGKSTTLATMVDYMNQKRREHILT
LEDPIEYLHKHKNSMVNQREIGFDTNSYANALRAALREDPDVILVGEMRDQETISTAITAAETGHLVLSTLHTIGAAQTI
DRIIDVFPPHQQQQIRVQLANILKGVVSQQLLPRCDIKGRCVALEIMLGNAAIANLIREEKTHQIYSQIQTGALTGMVTM
DASIVKHYKEGRISLETASHYAQIRDEFKRLIGR

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=40115 CLOLE_RS08740 WP_013656738.1 1939078..1940142(+) (pilT) [Cellulosilyticum lentocellum DSM 5427]
ATGGATGTTAATGATTTATTAGAAATTGTTGTAGAACAGAATGCTTCAGATATACATTTAACTGTAGGACTTCCGCCTAC
ACTCAGGTTACATGGTCAATTAACACCTTTAGATTTGCCTACTCTAAGAGAAGCAGATACAAAAGAACTTATAGGTCAGT
TACTAGAAGGAAAGCCTATTTTAATGGAGAAGTTTAGAGAAAAAGGAGAAGTAGACTTTTCTTATGCACTTCAAGATATA
GGACGTTTTCGTGTTAATGTATATAAGCAAAGGGGAAGTTGGGCAACAGCCCTTCGTTTGGTTGGCGTAGATATTCCGAG
AATGGAAGACCTAGGGCTACCACGAAAAATATTAGAATCTCTTTCTGATAATACAAGAGGGCTTATTTTAGTTACAGGAC
CTACAGGAAGTGGTAAATCTACTACTTTAGCCACTATGGTGGACTATATGAATCAAAAAAGAAGAGAACATATTTTGACA
CTTGAAGACCCTATTGAATATTTACATAAACATAAAAATAGTATGGTAAATCAGAGAGAAATTGGTTTTGATACCAATAG
CTATGCTAATGCTTTAAGAGCCGCTTTACGTGAAGACCCAGATGTCATTCTGGTAGGAGAAATGCGTGATCAAGAAACGA
TTTCTACAGCTATTACAGCAGCAGAAACGGGTCATTTGGTATTAAGTACCTTACATACCATTGGGGCAGCTCAGACCATA
GACCGTATTATAGACGTTTTTCCACCTCATCAGCAGCAGCAAATACGTGTACAGTTGGCTAATATTCTAAAGGGAGTTGT
TTCCCAGCAGCTTTTGCCAAGATGCGATATTAAGGGGAGATGTGTGGCACTTGAGATTATGTTAGGTAATGCGGCAATAG
CCAATTTGATTCGTGAGGAAAAGACGCATCAAATTTATTCTCAAATTCAAACAGGCGCATTAACAGGAATGGTTACAATG
GATGCTTCTATTGTAAAGCACTATAAAGAAGGTCGGATTTCACTAGAAACAGCTAGCCATTATGCACAAATCCGAGATGA
ATTTAAACGTCTAATAGGACGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2JMC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.997

99.153

0.506
  pilT Acinetobacter baylyi ADP1

49.138

98.305

0.483
  pilT Acinetobacter nosocomialis M2

48.851

98.305

0.48
  pilT Acinetobacter baumannii D1279779

48.851

98.305

0.48
  pilT Acinetobacter baumannii strain A118

48.851

98.305

0.48
  pilT Pseudomonas aeruginosa PAK

47.989

98.305

0.472
  pilT Legionella pneumophila strain ERS1305867

48.538

96.61

0.469
  pilT Legionella pneumophila strain Lp02

48.538

96.61

0.469
  pilT Vibrio cholerae strain A1552

51.713

90.678

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.713

90.678

0.469
  pilT Pseudomonas stutzeri DSM 10701

47.414

98.305

0.466
  pilT Neisseria meningitidis 8013

46.356

96.893

0.449
  pilT Neisseria gonorrhoeae MS11

46.064

96.893

0.446
  pilU Vibrio cholerae strain A1552

39.205

99.435

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.636

99.435

0.384
  pilU Acinetobacter baylyi ADP1

37.971

97.458

0.37

Multiple sequence alignment