Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GJ672_RS04175 Genome accession   NZ_CP046046
Coordinates   822538..823572 (+) Length   344 a.a.
NCBI ID   WP_154296017.1    Uniprot ID   A0A6B8JR72
Organism   Spiribacter sp. 2438     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 817538..828572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GJ672_RS04155 (GJ672_04155) - 819099..819527 (-) 429 WP_154296013.1 hypothetical protein -
  GJ672_RS04160 (GJ672_04160) gspM 819505..820218 (-) 714 WP_154296014.1 type II secretion system protein GspM -
  GJ672_RS04165 (GJ672_04165) - 820211..821761 (-) 1551 WP_154296015.1 hypothetical protein -
  GJ672_RS04170 (GJ672_04170) - 821873..822304 (-) 432 WP_154296016.1 type II secretion system protein -
  GJ672_RS04175 (GJ672_04175) pilT 822538..823572 (+) 1035 WP_154296017.1 type IV pilus twitching motility protein PilT Machinery gene
  GJ672_RS04180 (GJ672_04180) - 823649..824029 (-) 381 WP_154296018.1 helix-turn-helix domain-containing protein -
  GJ672_RS04190 (GJ672_04190) aceK 824327..826069 (-) 1743 WP_154296019.1 bifunctional isocitrate dehydrogenase kinase/phosphatase -
  GJ672_RS04195 (GJ672_04195) icd 826089..827390 (-) 1302 WP_370517606.1 NADP-dependent isocitrate dehydrogenase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 36790.85 Da        Isoelectric Point: 5.7136

>NTDB_id=400669 GJ672_RS04175 WP_154296017.1 822538..823572(+) (pilT) [Spiribacter sp. 2438]
MELNALISEAINHGASDLHVSAGVPPLARIDGELVSVDENAVTAKALERALLDVMDERQRAAYADHREVDFALQVEGAGR
FRVNVYEQLNGPGVVLRAIPEAIPSLESLGAPSVVQSLADRARGLVLVTGPTGSGKSTTLAAMVDHRNQTYPEHILTIED
PIEFVHPRQRALITQREVGHHTHDVHHALRAALREDPDVILVGEMRDPDTIRLALTAAETGHLVFGTLHTRTAASSVDRI
INTFPGEEQAQVRAMLAESLLAVISQSLLPRAGGGRVAAFEVMVAMPAIRNLIRESNIAQIPNALQTGQASGMQTMAQAV
QRLKQQGLISAATAEPIIGESGLG

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=400669 GJ672_RS04175 WP_154296017.1 822538..823572(+) (pilT) [Spiribacter sp. 2438]
ATGGAACTTAATGCCTTAATTTCAGAAGCGATTAACCATGGTGCGTCGGACCTGCATGTGTCCGCCGGAGTTCCTCCACT
CGCCCGAATCGATGGTGAGCTGGTGTCAGTGGATGAAAACGCGGTGACGGCGAAGGCCTTGGAACGGGCCCTGCTGGATG
TCATGGACGAGCGTCAGCGCGCGGCCTACGCCGATCACCGCGAGGTGGATTTTGCTCTGCAGGTGGAGGGCGCCGGGCGA
TTTCGGGTCAATGTCTATGAGCAGCTCAACGGGCCCGGGGTGGTGCTGCGGGCGATCCCGGAGGCGATTCCCTCGCTGGA
ATCCCTGGGGGCACCGTCGGTGGTGCAGTCACTGGCGGATCGGGCGCGGGGGCTGGTGCTGGTGACCGGGCCGACGGGGT
CTGGCAAGTCCACCACGCTGGCGGCGATGGTGGACCATCGCAATCAGACCTATCCCGAGCACATCCTCACCATTGAGGAT
CCCATTGAGTTCGTCCATCCCCGGCAGCGGGCGCTGATCACCCAGCGGGAGGTGGGGCACCACACCCACGATGTCCATCA
CGCCCTGCGGGCGGCGTTGCGCGAAGACCCGGATGTGATACTGGTGGGGGAAATGCGAGACCCGGACACCATCCGCCTGG
CCCTCACGGCGGCCGAGACCGGTCACCTGGTGTTCGGCACCCTGCACACGCGCACGGCGGCCAGCAGTGTGGACCGGATC
ATCAATACCTTTCCGGGGGAAGAGCAGGCCCAGGTGCGGGCCATGCTGGCCGAGTCACTGCTGGCGGTGATCTCCCAGTC
CCTACTACCCCGGGCGGGGGGCGGGCGCGTGGCGGCCTTCGAGGTCATGGTGGCCATGCCGGCGATCCGTAATCTGATTC
GGGAGAGCAATATCGCGCAGATCCCCAACGCGCTGCAGACCGGGCAGGCATCGGGCATGCAGACCATGGCCCAGGCGGTG
CAGCGACTGAAGCAGCAGGGGCTGATATCTGCCGCGACCGCCGAGCCAATTATTGGCGAGAGTGGCCTGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B8JR72

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.495

96.221

0.544
  pilT Vibrio cholerae strain A1552

56.495

96.221

0.544
  pilT Neisseria meningitidis 8013

55.522

97.384

0.541
  pilT Neisseria gonorrhoeae MS11

55.224

97.384

0.538
  pilT Acinetobacter nosocomialis M2

55.09

97.093

0.535
  pilT Acinetobacter baumannii D1279779

55.09

97.093

0.535
  pilT Acinetobacter baumannii strain A118

55.09

97.093

0.535
  pilT Acinetobacter baylyi ADP1

53.709

97.965

0.526
  pilT Pseudomonas aeruginosa PAK

53.892

97.093

0.523
  pilT Pseudomonas stutzeri DSM 10701

53.731

97.384

0.523
  pilT Legionella pneumophila strain ERS1305867

52.695

97.093

0.512
  pilT Legionella pneumophila strain Lp02

52.695

97.093

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.64

96.221

0.468
  pilU Vibrio cholerae strain A1552

43.188

100

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

40.774

97.674

0.398

Multiple sequence alignment