Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GHC23_RS03960 Genome accession   NZ_CP045840
Coordinates   819368..820348 (+) Length   326 a.a.
NCBI ID   WP_153752286.1    Uniprot ID   -
Organism   Citrobacter sp. S40-1-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 814368..825348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHC23_RS03930 ansB 814883..815929 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  GHC23_RS03935 hemW 816044..817180 (-) 1137 WP_137377727.1 radical SAM family heme chaperone HemW -
  GHC23_RS03940 - 817173..817766 (-) 594 WP_153752284.1 XTP/dITP diphosphatase -
  GHC23_RS03945 yggU 817774..818064 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  GHC23_RS03950 - 818061..818627 (-) 567 WP_003825417.1 YggT family protein -
  GHC23_RS03955 - 818646..819350 (-) 705 WP_153752285.1 YggS family pyridoxal phosphate-dependent enzyme -
  GHC23_RS03960 pilT 819368..820348 (+) 981 WP_153752286.1 type IV pilus twitching motility protein PilT Machinery gene
  GHC23_RS03965 ruvX 820345..820761 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  GHC23_RS03970 - 820761..821324 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  GHC23_RS03975 gshB 821501..822448 (-) 948 WP_003027083.1 glutathione synthase -
  GHC23_RS03980 rsmE 822468..823199 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  GHC23_RS03985 endA 823274..823981 (-) 708 WP_153752287.1 deoxyribonuclease I -
  GHC23_RS03990 - 824076..824573 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35951.13 Da        Isoelectric Point: 6.3013

>NTDB_id=398580 GHC23_RS03960 WP_153752286.1 819368..820348(+) (pilT) [Citrobacter sp. S40-1-2]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQLVQWQEQGQIDFAIALAGSRRLRA
SAFVHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQQVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=398580 GHC23_RS03960 WP_153752286.1 819368..820348(+) (pilT) [Citrobacter sp. S40-1-2]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACTGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGGTTCCCGGCGGCTGCGCGCC
AGTGCTTTTGTTCATCAACAGGGAACCTCACTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTTGAGTACCTTAATCAGCAAGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTGCACTGCGCTTCTTTTGCAGCTGGTTTACG
CGGCGCGTTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAGCTACGCGACGTGGAAACCATTCGTCTGGCGTTAACCG
CAGCGGAAACTGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGGCGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baylyi ADP1

45.593

100

0.46
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374
  pilU Acinetobacter baylyi ADP1

37.037

99.387

0.368

Multiple sequence alignment