Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BR63_RS03775 Genome accession   NZ_CP045798
Coordinates   733568..734647 (+) Length   359 a.a.
NCBI ID   WP_034425503.1    Uniprot ID   A0A7G6E0A4
Organism   Thermanaerosceptrum fracticalcis strain DRI-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728568..739647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BR63_RS03740 (BR63_03720) - 729011..729223 (+) 213 WP_081908323.1 sigma-70 family RNA polymerase sigma factor -
  BR63_RS03745 (BR63_03725) - 729348..729584 (+) 237 WP_034425500.1 hypothetical protein -
  BR63_RS03750 (BR63_03730) - 729827..730312 (+) 486 WP_051966230.1 YqeG family HAD IIIA-type phosphatase -
  BR63_RS03755 (BR63_03735) - 730366..730509 (-) 144 WP_153802185.1 hypothetical protein -
  BR63_RS03760 (BR63_03740) - 730721..731191 (+) 471 WP_051966231.1 MerR family transcriptional regulator -
  BR63_RS03765 (BR63_03745) - 731206..731778 (+) 573 WP_276568978.1 shikimate kinase -
  BR63_RS03770 (BR63_03750) - 731870..733564 (+) 1695 WP_034425501.1 GspE/PulE family protein -
  BR63_RS03775 (BR63_03755) pilT 733568..734647 (+) 1080 WP_034425503.1 type IV pilus twitching motility protein PilT Machinery gene
  BR63_RS03780 (BR63_03760) - 734869..736086 (+) 1218 WP_034425538.1 type II secretion system F family protein -
  BR63_RS03785 (BR63_03765) - 736257..736676 (+) 420 WP_051966237.1 competence type IV pilus major pilin ComGC -
  BR63_RS03790 (BR63_03770) - 736733..737476 (+) 744 WP_034425505.1 prepilin peptidase -
  BR63_RS03795 (BR63_03775) - 737487..737939 (+) 453 WP_034425507.1 GspH/FimT family pseudopilin -
  BR63_RS03800 (BR63_03780) - 737955..738326 (+) 372 WP_034425510.1 type IV pilus modification PilV family protein -
  BR63_RS03805 (BR63_03785) - 738319..738828 (+) 510 WP_034425514.1 PilW family protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39315.17 Da        Isoelectric Point: 6.2510

>NTDB_id=397272 BR63_RS03775 WP_034425503.1 733568..734647(+) (pilT) [Thermanaerosceptrum fracticalcis strain DRI-13]
MDVIELLKSAVKAGASDIHLSVGAYPMFRINGQLKRWVPEEGENLPLKREDTLKAAEILMNSEQMEAWQQKGELDFSYSL
PGVGRFRVNIYRQRGCVSLALRPVPYQIPSLESLGVPQAVVGLADKTNGLVLVTGPTGSGKSTTLAALINKINQERACHI
ITIEDPIEYLYQHQKSVVDQRELGSDTFSLAGALRACLRQDPDVILVGEMRDLETIATAITAAETGHLVFATLHTNSAAQ
SVDRIIDVFPPGQQEQIKVQLAATLQGVVTQQLLPRADGMGRVLAAEVMIVTPAVRNLIREGKTHQIQTVLQTGGRWGMQ
TMDMALRELVRSGAVTMETALKYTNDQENFSRLVNATGF

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=397272 BR63_RS03775 WP_034425503.1 733568..734647(+) (pilT) [Thermanaerosceptrum fracticalcis strain DRI-13]
ATGGATGTGATAGAATTATTAAAGAGTGCGGTTAAAGCTGGGGCTTCAGATATTCACCTCTCCGTAGGCGCTTATCCCAT
GTTTCGTATTAATGGTCAGTTGAAGAGATGGGTGCCGGAAGAAGGAGAAAACCTGCCCTTAAAAAGGGAAGACACGCTAA
AAGCGGCGGAAATCCTCATGAATTCGGAACAAATGGAAGCCTGGCAGCAAAAAGGAGAACTGGATTTTTCTTATTCTCTG
CCTGGCGTGGGGAGATTCAGGGTTAATATATACCGCCAGCGGGGCTGTGTGAGCCTGGCCCTGCGGCCTGTGCCTTACCA
GATTCCTTCCCTGGAGAGCCTAGGGGTTCCCCAGGCTGTAGTGGGTCTGGCCGATAAAACCAATGGGCTGGTACTGGTCA
CGGGACCAACAGGCAGCGGCAAATCCACTACTTTAGCTGCCCTCATTAACAAAATCAACCAGGAGCGGGCCTGTCATATC
ATTACCATTGAAGACCCCATCGAGTATCTTTACCAACATCAAAAAAGTGTAGTGGACCAGAGGGAACTGGGGAGCGATAC
CTTTTCCCTGGCCGGCGCCCTGCGGGCCTGCCTGCGCCAGGACCCGGACGTTATCCTGGTAGGTGAAATGCGGGACCTGG
AGACTATTGCCACGGCCATTACTGCCGCGGAGACAGGCCACCTGGTCTTTGCCACCCTCCATACCAACAGTGCCGCCCAG
TCTGTGGACCGGATTATTGACGTCTTTCCACCCGGCCAGCAGGAACAGATCAAGGTCCAGCTTGCGGCTACCCTGCAGGG
CGTCGTAACCCAGCAGCTCCTGCCCAGGGCCGATGGCATGGGCAGAGTTTTAGCGGCGGAGGTAATGATTGTCACTCCTG
CGGTCCGTAACCTAATCCGGGAAGGTAAGACCCACCAGATTCAGACTGTGCTGCAGACAGGCGGGCGCTGGGGGATGCAG
ACCATGGATATGGCTCTGCGCGAGCTTGTGCGTTCCGGGGCAGTGACTATGGAAACGGCGCTTAAGTATACCAATGACCA
GGAAAATTTTAGCCGCCTGGTGAATGCAACGGGATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G6E0A4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.521

98.886

0.529
  pilT Neisseria meningitidis 8013

50.568

98.05

0.496
  pilT Neisseria gonorrhoeae MS11

50.284

98.05

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.445

93.872

0.474
  pilT Vibrio cholerae strain A1552

50.445

93.872

0.474
  pilT Pseudomonas aeruginosa PAK

48.148

97.772

0.471
  pilT Acinetobacter nosocomialis M2

48.148

97.772

0.471
  pilT Acinetobacter baumannii D1279779

48.148

97.772

0.471
  pilT Acinetobacter baumannii strain A118

48.148

97.772

0.471
  pilT Legionella pneumophila strain ERS1305867

49.267

94.986

0.468
  pilT Legionella pneumophila strain Lp02

49.267

94.986

0.468
  pilT Pseudomonas stutzeri DSM 10701

47.578

97.772

0.465
  pilT Acinetobacter baylyi ADP1

47.293

97.772

0.462
  pilU Pseudomonas stutzeri DSM 10701

41.899

99.721

0.418
  pilU Vibrio cholerae strain A1552

39.216

99.443

0.39
  pilU Acinetobacter baylyi ADP1

37.322

97.772

0.365

Multiple sequence alignment