Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THEBR_RS05435 Genome accession   NC_014964
Coordinates   1123048..1124097 (+) Length   349 a.a.
NCBI ID   WP_012269299.1    Uniprot ID   A0A1G7HDU7
Organism   Thermoanaerobacter brockii subsp. finnii Ako-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1118048..1129097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEBR_RS05415 (Thebr_1091) sigK 1119149..1119844 (+) 696 WP_003868463.1 RNA polymerase sporulation sigma factor SigK -
  THEBR_RS05420 (Thebr_1092) - 1119908..1120408 (+) 501 WP_009052315.1 YqeG family HAD IIIA-type phosphatase -
  THEBR_RS05425 (Thebr_1093) aroE 1120398..1121252 (+) 855 WP_012269298.1 shikimate dehydrogenase -
  THEBR_RS05430 (Thebr_1094) gspE 1121366..1123042 (+) 1677 WP_009052317.1 type II secretion system ATPase GspE -
  THEBR_RS05435 (Thebr_1095) pilT 1123048..1124097 (+) 1050 WP_012269299.1 type IV pilus twitching motility protein PilT Machinery gene
  THEBR_RS05440 (Thebr_1096) - 1124108..1125319 (+) 1212 WP_012268712.1 type II secretion system F family protein -
  THEBR_RS05445 (Thebr_1097) - 1125344..1125709 (+) 366 WP_003868457.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEBR_RS05450 (Thebr_1098) - 1125777..1126535 (+) 759 WP_003868456.1 A24 family peptidase -
  THEBR_RS05455 (Thebr_1099) - 1126540..1126998 (+) 459 WP_003868455.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEBR_RS05460 (Thebr_1100) - 1127009..1127398 (+) 390 WP_003868454.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEBR_RS05465 (Thebr_1101) - 1127494..1128006 (+) 513 WP_039913350.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  THEBR_RS05470 (Thebr_1102) - 1127996..1128655 (+) 660 WP_009052323.1 pilus assembly PilX N-terminal domain-containing protein -
  THEBR_RS05475 (Thebr_1103) - 1128674..1128934 (+) 261 WP_003868451.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38641.90 Da        Isoelectric Point: 7.2222

>NTDB_id=39640 THEBR_RS05435 WP_012269299.1 1123048..1124097(+) (pilT) [Thermoanaerobacter brockii subsp. finnii Ako-1]
MDTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTPGQTEEIVKDLLTSEQLKKLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPPILKDLALKARGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAASYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEVMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKRGVISLEDALTYCVDQENFSRMI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=39640 THEBR_RS05435 WP_012269299.1 1123048..1124097(+) (pilT) [Thermoanaerobacter brockii subsp. finnii Ako-1]
ATGGATACAAAGGAACTTTTAAAATTAGTAGTTGAAAGAAAAGCTTCAGACTTGCATATAACGGTTGGAGTACCTCCAGT
TCTTAGAATTAATGGCTATTTAGAAAAATTAGAAGGGGAACCTTTTACCCCTGGGCAGACTGAAGAAATAGTTAAGGATT
TATTGACAAGTGAGCAATTGAAAAAGTTAGAACAAAATGGAGATATTGATTTGTCCTATTCAGTTACAGGATTAGGAAGG
TTTAGAATAAATGTATACAAACAAAGGGGAAGTTATAGCCTTGCAATAAGGTCTGTAGCTTTGAGAATACCTACAATAGA
GGAATTGGGACTTCCTCCAATACTTAAAGACTTAGCTTTAAAGGCAAGAGGGCTTATATTGGTAACAGGACCTACTGGCA
GTGGTAAATCTACTACACTGGCTGCGATGGTGGATTGGATAAACTCTAAAAGGACTTGTCATATTCTCACATTGGAAGAC
CCTATAGAATATTTACATAAACACAACAAAAGCATAGTAAATCAAAGAGAGATTGGGCATGATGCGGCATCTTACGCTTC
AGCTTTAAGGGCTGCTTTAAGGGAGGACCCGGATGTTATATTAGTGGGAGAGATGAGGGATTTAGAAACCATCCAAATTG
CTATAACTGCTGCTGAGACAGGCCATCTTGTTTTGTCCACTCTTCACACAATAGGAGCCGTTAAGACGATCGATAGGATT
ATTGATGTTTTTCCTCCCCATCAGCAGCAGCAGATAAAAATTCAGCTTTCAAATGTTTTAGAGGGAATTATTTCTCAACA
GCTTATACCTAAAAAGGACAACAGCGGCAGAGTAGTTGCGACAGAAGTGATGATTGCTACTCCTGCTATAAGAAATCTCA
TAAGAGAAGGGAAGACTTTCCAGATACAGTCGGCAGTTCAAACAGGTAGCAAGTTTGGAATGATGACAATGGATATGTCT
ATTTTACATCTGTTAAAAAGAGGAGTTATATCATTAGAGGATGCTTTGACATATTGCGTAGACCAAGAGAATTTTTCTCG
CATGATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G7HDU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

49.565

98.854

0.49
  pilT Acinetobacter nosocomialis M2

49.275

98.854

0.487
  pilT Acinetobacter baumannii D1279779

49.275

98.854

0.487
  pilT Pseudomonas aeruginosa PAK

49.275

98.854

0.487
  pilT Acinetobacter baumannii strain A118

49.275

98.854

0.487
  pilT Legionella pneumophila strain ERS1305867

48.986

98.854

0.484
  pilT Legionella pneumophila strain Lp02

48.986

98.854

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.701

99.713

0.476
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

95.702

0.473
  pilT Vibrio cholerae strain A1552

49.401

95.702

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.536

98.854

0.47
  pilT Neisseria meningitidis 8013

45.954

99.14

0.456
  pilT Neisseria gonorrhoeae MS11

45.665

99.14

0.453
  pilU Pseudomonas stutzeri DSM 10701

41.834

100

0.418
  pilU Vibrio cholerae strain A1552

40.401

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

99.14

0.39

Multiple sequence alignment