Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HPM96_RS18095 Genome accession   NZ_CP053852
Coordinates   3762965..3763945 (-) Length   326 a.a.
NCBI ID   WP_001361345.1    Uniprot ID   -
Organism   Escherichia coli strain CE1628     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3757965..3768945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPM96_RS18065 (HPM96_18060) yggI 3758629..3759126 (+) 498 WP_023908971.1 SprT family zinc-dependent metalloprotease -
  HPM96_RS18070 (HPM96_18065) endA 3759221..3759928 (+) 708 WP_000286500.1 deoxyribonuclease I -
  HPM96_RS18075 (HPM96_18070) rsmE 3760008..3760739 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  HPM96_RS18080 (HPM96_18075) gshB 3760752..3761702 (+) 951 WP_000593278.1 glutathione synthase -
  HPM96_RS18085 (HPM96_18080) yqgE 3761811..3762374 (+) 564 WP_001339287.1 YqgE/AlgH family protein -
  HPM96_RS18090 (HPM96_18085) ruvX 3762374..3762790 (+) 417 WP_000017100.1 Holliday junction resolvase RuvX -
  HPM96_RS18095 (HPM96_18090) pilT 3762965..3763945 (-) 981 WP_001361345.1 type IV pilus twitching motility protein PilT Machinery gene
  HPM96_RS18100 (HPM96_18095) yggS 3763963..3764667 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  HPM96_RS18105 (HPM96_18100) yggT 3764685..3765251 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  HPM96_RS18110 (HPM96_18105) yggU 3765248..3765538 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  HPM96_RS18115 (HPM96_18110) rdgB 3765546..3766139 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  HPM96_RS18120 (HPM96_18115) hemW 3766132..3767268 (+) 1137 WP_000239988.1 radical SAM family heme chaperone HemW -
  HPM96_RS18125 (HPM96_18120) - 3767581..3768567 (+) 987 WP_000784004.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35970.18 Da        Isoelectric Point: 5.7980

>NTDB_id=395893 HPM96_RS18095 WP_001361345.1 3762965..3763945(-) (pilT) [Escherichia coli strain CE1628]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAVPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=395893 HPM96_RS18095 WP_001361345.1 3762965..3763945(-) (pilT) [Escherichia coli strain CE1628]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGTGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CAGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGACGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.694

97.239

0.396
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

37.267

98.773

0.368