Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DEIMA_RS00110 Genome accession   NC_014958
Coordinates   23049..24269 (-) Length   406 a.a.
NCBI ID   WP_013555190.1    Uniprot ID   E8U3Z1
Organism   Deinococcus maricopensis DSM 21211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 18049..29269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEIMA_RS00085 (Deima_0016) - 18676..19620 (-) 945 WP_013555185.1 DNA polymerase III -
  DEIMA_RS00090 (Deima_0017) - 19646..20395 (-) 750 WP_013555186.1 metallophosphoesterase family protein -
  DEIMA_RS00095 (Deima_0018) - 20445..21155 (+) 711 WP_013555187.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  DEIMA_RS00100 (Deima_0019) - 21152..21649 (+) 498 WP_013555188.1 hypothetical protein -
  DEIMA_RS00105 (Deima_0020) hemW 21749..22903 (+) 1155 WP_013555189.1 radical SAM family heme chaperone HemW -
  DEIMA_RS00110 (Deima_0021) pilT 23049..24269 (-) 1221 WP_013555190.1 type IV pilus twitching motility protein PilT Machinery gene
  DEIMA_RS00115 (Deima_0022) pilB 24314..26977 (-) 2664 WP_013555191.1 type II/IV secretion system protein Machinery gene
  DEIMA_RS00120 (Deima_0023) - 26981..27505 (-) 525 WP_013555192.1 YqeG family HAD IIIA-type phosphatase -
  DEIMA_RS00125 (Deima_0024) pgeF 27502..28317 (-) 816 WP_013555193.1 peptidoglycan editing factor PgeF -
  DEIMA_RS00130 (Deima_0025) - 28444..29235 (+) 792 WP_043816307.1 enoyl-ACP reductase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44274.57 Da        Isoelectric Point: 7.0803

>NTDB_id=39588 DEIMA_RS00110 WP_013555190.1 23049..24269(-) (pilT) [Deinococcus maricopensis DSM 21211]
MATDITDILRVAAEKQASDVIITAGLPPQFKLHGEYTGLDFGPLEATDTRKLMYSMMNEKQQRTFEEKRELDFSFALGDK
ARFRVNTFVQRGAVGGVLRLIPTKIKSAEDLGLPKTVVDIANAPRGLVLVTGPTGSGKSTTLAAMIDWINLNKKLHIMTI
EDPIEFMHPHKNSIVNQREIGADTMDFQAALRAVLRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAPESID
RIVDVFPEEQQEQIRVQLANNLVAVMTQQLIPRADGGRILAYELLIANPAVRSLIREGKTFQITSSMQTGAKEGMITMDA
FLANLYKRRLITYDAGLERAVDAKEYARLAQDASATASSMPTGYGQQTFGQATPGTTAPNNNLGRGGATTPTSTATPNPT
NPYGRR

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=39588 DEIMA_RS00110 WP_013555190.1 23049..24269(-) (pilT) [Deinococcus maricopensis DSM 21211]
ATGGCCACCGACATCACCGATATCCTGCGCGTCGCCGCCGAGAAGCAAGCCTCGGACGTCATCATCACTGCCGGCCTGCC
GCCGCAGTTCAAGCTGCACGGCGAGTACACCGGCCTCGACTTCGGCCCGCTCGAAGCGACCGACACCCGCAAGCTGATGT
ACTCGATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAGAAGCGCGAACTGGACTTCAGCTTCGCGCTGGGCGACAAG
GCCCGCTTCCGCGTGAACACGTTCGTGCAGCGCGGCGCGGTCGGCGGCGTGCTCCGTCTGATCCCCACGAAGATTAAGAG
CGCCGAGGACCTCGGTCTGCCCAAGACCGTCGTCGACATCGCCAACGCCCCGCGCGGCCTGGTCCTCGTGACCGGCCCCA
CCGGCTCTGGCAAGAGCACCACGCTCGCCGCGATGATCGACTGGATCAACCTCAACAAGAAGCTGCACATCATGACGATC
GAGGACCCGATCGAGTTCATGCACCCGCACAAGAACAGCATCGTGAACCAGCGCGAGATCGGCGCGGACACCATGGACTT
CCAGGCGGCGCTGCGCGCCGTGCTGCGTCAGGCGCCCGACGTGATCCTCGTGGGCGAAATGCGTGACTACGAAACCATCA
AGGCCGCCGTGACCGCCGCGGAAACCGGCCACCTCGTGATGGGCACGCTGCACACCAACAGCGCGCCGGAAAGCATCGAC
CGTATCGTGGACGTGTTCCCGGAAGAGCAGCAGGAGCAGATCCGCGTGCAGCTCGCGAACAACCTCGTGGCCGTGATGAC
GCAGCAGCTGATTCCCCGCGCGGACGGCGGGCGCATCCTCGCGTACGAGCTGCTCATCGCGAACCCGGCCGTGCGGTCCC
TGATTCGCGAAGGGAAGACCTTCCAGATCACCAGCAGCATGCAGACCGGCGCGAAGGAAGGCATGATCACCATGGACGCG
TTCCTGGCGAACCTGTACAAGCGTCGCCTGATCACGTACGACGCGGGCCTGGAGCGCGCCGTGGACGCCAAGGAGTACGC
GCGTCTCGCGCAGGACGCGTCCGCGACGGCGTCGTCCATGCCGACCGGATACGGGCAGCAGACGTTCGGGCAGGCGACGC
CCGGCACGACCGCGCCGAACAACAACCTGGGGCGCGGCGGCGCCACAACGCCGACGTCCACGGCCACGCCGAACCCCACC
AACCCGTACGGTCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(39-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E8U3Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

76.486

95.32

0.729
  pilT Acinetobacter baumannii D1279779

52.584

81.034

0.426
  pilT Acinetobacter baumannii strain A118

52.584

81.034

0.426
  pilT Acinetobacter nosocomialis M2

52.584

81.034

0.426
  pilT Pseudomonas stutzeri DSM 10701

51.335

83.005

0.426
  pilT Pseudomonas aeruginosa PAK

51.335

83.005

0.426
  pilT Acinetobacter baylyi ADP1

52.134

80.788

0.421
  pilT Neisseria meningitidis 8013

49.565

84.975

0.421
  pilT Legionella pneumophila strain Lp02

49.853

83.498

0.416
  pilT Legionella pneumophila strain ERS1305867

49.853

83.498

0.416
  pilT Neisseria gonorrhoeae MS11

49.128

84.729

0.416
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.085

80.788

0.397
  pilT Vibrio cholerae strain A1552

49.085

80.788

0.397
  pilU Pseudomonas stutzeri DSM 10701

44.444

88.67

0.394
  pilU Acinetobacter baylyi ADP1

42.857

87.931

0.377

Multiple sequence alignment