Detailed information NTG0039589

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DEIMA_RS00115
• Genome accession: NC_014958
• Coordinates: 24314..26977 (-)
• Length: 887 a.a.
• NCBI ID: WP_013555191.1
• Uniprot ID: E8U3Z2
• Organism: Deinococcus maricopensis DSM 21211
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 19314..31977

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DEIMA_RS00090 (Deima_0017)	-	19646..20395 (-)	750	WP_013555186.1	metallophosphoesterase family protein	-
DEIMA_RS00095 (Deima_0018)	-	20445..21155 (+)	711	WP_013555187.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
DEIMA_RS00100 (Deima_0019)	-	21152..21649 (+)	498	WP_013555188.1	hypothetical protein	-
DEIMA_RS00105 (Deima_0020)	hemW	21749..22903 (+)	1155	WP_013555189.1	radical SAM family heme chaperone HemW	-
DEIMA_RS00110 (Deima_0021)	pilT	23049..24269 (-)	1221	WP_013555190.1	type IV pilus twitching motility protein PilT	Machinery gene
DEIMA_RS00115 (Deima_0022)	pilB	24314..26977 (-)	2664	WP_013555191.1	type II/IV secretion system protein	Machinery gene
DEIMA_RS00120 (Deima_0023)	-	26981..27505 (-)	525	WP_013555192.1	YqeG family HAD IIIA-type phosphatase	-
DEIMA_RS00125 (Deima_0024)	pgeF	27502..28317 (-)	816	WP_013555193.1	peptidoglycan editing factor PgeF	-
DEIMA_RS00130 (Deima_0025)	-	28444..29235 (+)	792	WP_043816307.1	enoyl-ACP reductase	-
DEIMA_RS00135 (Deima_0026)	-	29304..30341 (+)	1038	WP_148234842.1	M42 family metallopeptidase	-
DEIMA_RS00140 (Deima_0027)	-	30424..30624 (+)	201	WP_013555196.1	DUF2945 domain-containing protein	-
DEIMA_RS00145 (Deima_0029)	kynA	31007..31840 (+)	834	WP_013555198.1	tryptophan 2,3-dioxygenase	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 97367.65 Da
• Isoelectric Point: 4.8175

>NTDB_id=39589 DEIMA_RS00115 WP_013555191.1 24314..26977(-) (pilB) [Deinococcus maricopensis DSM 21211]
MALSIGDRRLGAILLEQGYVNDTDLQRALDRHSEVGGRLADILIDSSMVGEKRIARAVEEALGIPLVSLTQLEPDPKAIA
AVPAEAAQASQAFPFALDGATLRVAFVDPLSSMNIETIEDESGYAVEPYQALRSHLNWAIAEHYPELGLNAQKPIEAGEQ
VSLLGQKMLQRGLITDAQINQALELQKQTGDPLGAALIELGFITEDQLYETLAEQSNTVFVRNPRDYEPDESVLGLMLRA
DALRLTSVPVRDQGDHVLVITSDQRRRADLESIIGRPVQLALAKPADVEMLIDRLYPQRGRLGEAMVNAGTLSRDQLREA
LQVQARSGKVKPLGEVIVDLGFAEADEISEAIEKQNTGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEYLDPQQSPPDPK
VAQLIPEATARRFVVVPVRMQGDALVVAMKDPRNVFALDDLRLIAGREIIPAVMAERDIIRLIERYFGSNDMAELNKKLA
ETGRTREREREDADLNASLDDNAVVRVVDSIIREAALQDVSDIHIEPTEHNVKVRYRIDGDMRDHMELPKAAGPAIAARI
KILGNLDIAERRIPQDGRVRFRRGSIDLDLRLSTLPTVYGEKSVMRLLQKASNIPEVEQLGFSDHNFQRFLDTIEKPYGI
FLITGPTGSGKSFSSFSILKRIATSDKNTTTVEDPVEYEIPGINQTQVNTTAGLTFARALRAFLRQDPDIIFVGEIRDAE
TAKIATEAALTGHLVLATLHTNDAPGAVTRLEEMGVEHFNISAALIGVLAQRLVRRICQECKTETNADPDTLRRLGLRDE
ELQGATLYHGTGCNRCGGTGYKGRTAIHELMVVDDPLRRAIGGGKSAAEIRDVAVEQSNMRTLRQDGIEKALRGITTLEE
VLGSTSN

Nucleotide

• Length: 2664 bp

>NTDB_id=39589 DEIMA_RS00115 WP_013555191.1 24314..26977(-) (pilB) [Deinococcus maricopensis DSM 21211]
GTGGCACTCTCCATTGGTGACAGACGCCTGGGCGCCATCCTGCTGGAGCAGGGGTACGTCAACGACACGGACCTGCAGCG
CGCCCTGGACCGGCACTCCGAAGTCGGTGGCCGCCTCGCGGACATCCTGATCGACAGCAGCATGGTCGGCGAGAAACGCA
TCGCGCGCGCCGTCGAGGAAGCCCTCGGCATTCCGCTCGTGAGCCTCACGCAGCTCGAACCGGACCCCAAAGCGATCGCC
GCCGTGCCCGCCGAGGCCGCGCAGGCGTCGCAGGCGTTCCCGTTCGCGCTTGACGGCGCCACGCTGCGCGTCGCGTTCGT
CGACCCGCTCAGCAGCATGAACATCGAAACCATCGAGGATGAGAGCGGCTACGCCGTCGAGCCGTACCAGGCGCTGCGCT
CGCACCTCAACTGGGCCATCGCCGAGCACTACCCGGAACTGGGCCTGAACGCCCAGAAGCCCATCGAGGCCGGCGAGCAG
GTGTCGCTGCTCGGTCAGAAGATGCTGCAGCGCGGCCTGATCACCGACGCGCAGATCAACCAGGCGCTCGAACTGCAGAA
GCAGACGGGCGACCCGCTCGGCGCGGCCCTGATCGAACTCGGGTTCATCACTGAAGATCAGCTGTACGAAACGCTCGCCG
AGCAGAGCAACACGGTGTTCGTCCGCAACCCCCGCGACTACGAACCCGACGAGAGCGTCCTCGGGCTGATGCTGCGCGCC
GACGCGCTGCGCCTCACGTCCGTGCCCGTCCGCGACCAGGGCGACCACGTGCTCGTCATCACCAGCGACCAGCGCCGCCG
CGCGGACCTCGAATCGATCATCGGGCGGCCCGTACAGCTCGCGCTCGCCAAACCCGCCGACGTCGAAATGCTCATCGACC
GCCTGTACCCGCAGCGGGGCCGCCTGGGCGAAGCCATGGTGAACGCCGGCACACTCTCCCGCGACCAGCTGCGTGAGGCG
CTGCAGGTGCAGGCCCGCAGCGGCAAGGTCAAACCCCTCGGCGAGGTCATCGTCGACCTGGGCTTCGCGGAAGCCGACGA
GATCAGCGAAGCCATCGAGAAGCAGAACACCGGCGGGGGCCGCCTCGAAGACACCCTGGTGCAGAGCGGCAAGCTCAGCC
CGGAAATGCTCGCGCGCTCCCTGGCCGCGCAGCTCGGGTACGAGTACCTCGACCCGCAGCAGAGCCCCCCGGACCCGAAA
GTCGCGCAGCTGATTCCCGAAGCGACCGCGCGCCGCTTCGTGGTCGTGCCGGTGCGCATGCAGGGCGACGCGCTCGTCGT
CGCCATGAAAGACCCCCGCAACGTGTTCGCGCTCGACGACCTGCGCCTCATCGCGGGCCGCGAGATCATCCCGGCGGTCA
TGGCGGAACGCGACATCATCCGCCTGATCGAGCGGTACTTCGGGTCGAACGACATGGCCGAGCTGAACAAGAAGCTCGCC
GAAACCGGCCGCACCCGCGAACGCGAACGCGAGGACGCGGACCTGAACGCCAGCCTCGACGACAACGCCGTCGTGCGCGT
CGTCGACAGCATCATCCGCGAGGCGGCGCTGCAGGACGTCAGCGACATCCACATCGAGCCGACCGAACACAACGTGAAGG
TCCGCTACCGCATCGACGGTGACATGCGCGACCACATGGAACTGCCCAAGGCGGCCGGCCCGGCCATCGCGGCGCGCATC
AAGATTCTCGGGAACCTCGACATCGCCGAGCGGCGCATCCCCCAGGACGGCCGCGTGCGCTTCCGGCGCGGCAGCATCGA
CCTCGACCTGCGTCTCAGCACCCTCCCGACGGTGTACGGCGAGAAGTCCGTGATGCGTCTGCTGCAGAAGGCCAGCAACA
TCCCGGAAGTCGAGCAGCTGGGCTTCAGCGACCACAACTTCCAGCGCTTCCTCGACACCATCGAGAAACCGTACGGCATC
TTCCTGATCACCGGCCCGACCGGCTCGGGGAAGTCGTTCAGCAGCTTCTCGATCCTGAAGCGCATCGCCACGAGCGACAA
GAACACCACCACCGTCGAGGACCCCGTCGAGTACGAGATCCCCGGCATCAACCAGACGCAGGTGAACACCACCGCCGGCC
TGACCTTCGCCCGCGCGCTGCGCGCGTTCCTGCGTCAGGACCCGGACATTATCTTCGTTGGTGAGATCCGCGACGCCGAA
ACCGCGAAGATCGCCACCGAAGCGGCCCTCACCGGCCACCTGGTGCTCGCCACCCTCCACACCAACGACGCGCCCGGCGC
CGTCACCCGCCTGGAGGAGATGGGCGTGGAGCACTTCAACATCAGCGCCGCGCTGATCGGCGTGCTCGCGCAGCGCCTCG
TGCGCCGCATCTGCCAGGAGTGCAAGACCGAAACGAACGCCGACCCGGACACCCTGCGCCGCCTGGGGCTCAGGGACGAG
GAGTTGCAGGGCGCCACCCTCTACCACGGCACCGGCTGCAACCGTTGCGGCGGCACCGGGTACAAGGGCCGCACCGCCAT
TCACGAGCTGATGGTCGTCGACGACCCGCTGCGCCGCGCGATCGGCGGCGGCAAGAGCGCCGCGGAGATCCGCGACGTTG
CCGTCGAGCAGAGCAACATGCGCACCCTCCGTCAGGACGGCATCGAGAAGGCCCTGCGCGGCATCACCACCCTCGAAGAA
GTCCTCGGCAGCACCAGCAACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (511-776)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (197-300)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (366-469)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

3D structure

• AlphaFold DB: E8U3Z2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	70.562	100	0.708
pilF	Thermus thermophilus HB27	57.127	100	0.574