Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NITSA_RS08350 Genome accession   NC_014935
Coordinates   1647606..1648661 (+) Length   351 a.a.
NCBI ID   WP_013554587.1    Uniprot ID   -
Organism   Nitratifractor salsuginis DSM 16511     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1642606..1653661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NITSA_RS08320 (Nitsa_1646) - 1643357..1643815 (-) 459 WP_013554581.1 four helix bundle protein -
  NITSA_RS08325 (Nitsa_1647) rho 1643802..1645133 (-) 1332 WP_013554582.1 transcription termination factor Rho -
  NITSA_RS08330 (Nitsa_1648) - 1645274..1645672 (-) 399 WP_013554583.1 hypothetical protein -
  NITSA_RS08335 (Nitsa_1649) dapE 1645672..1646766 (-) 1095 WP_013554584.1 succinyl-diaminopimelate desuccinylase -
  NITSA_RS08340 (Nitsa_1650) - 1646753..1647124 (-) 372 WP_013554585.1 rhodanese-like domain-containing protein -
  NITSA_RS08345 (Nitsa_1651) gatC 1647292..1647582 (+) 291 WP_013554586.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  NITSA_RS08350 (Nitsa_1652) pilT 1647606..1648661 (+) 1056 WP_013554587.1 type IV pilus twitching motility protein PilT Machinery gene
  NITSA_RS08355 (Nitsa_1653) - 1648682..1649179 (+) 498 WP_013554588.1 CvpA family protein -
  NITSA_RS08360 (Nitsa_1654) - 1649189..1649674 (+) 486 WP_013554589.1 Fur family transcriptional regulator -
  NITSA_RS08365 (Nitsa_1655) lysS 1649671..1651233 (+) 1563 WP_013554590.1 lysine--tRNA ligase -
  NITSA_RS08370 (Nitsa_1656) - 1651235..1652485 (+) 1251 WP_013554591.1 serine hydroxymethyltransferase -
  NITSA_RS08375 (Nitsa_1657) - 1652466..1653005 (+) 540 WP_013554592.1 DUF1882 domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39088.23 Da        Isoelectric Point: 6.8894

>NTDB_id=39585 NITSA_RS08350 WP_013554587.1 1647606..1648661(+) (pilT) [Nitratifractor salsuginis DSM 16511]
MAFNIKKLLQSVVVHKASDLHLIAKKEPLLRVDGKLRPINMPKLTGEDIEEMCYGLLTEKQKKHFEEENELDFAVELEGV
GRFRVNYYRTLGDMAAAFRIIPLSVPTLDEIDAPPVYRKIIRREKGLILVTGPTGSGKSTTLAAMLNEINLTESKHIITV
EDPVEFVHQHKKCVFSHRSVGEDTKSFAAALKYAMREDPDIILIGEMRDKETIAAALTAAETGHLVFATLHTNSAPGTVN
RIIDVFSGDEQPQIRAMLSSSLVAVISQALLPKIGGGRVAVPEILITNHAIANLIREDKVHQIYSAMQLGQEDSGMQTQT
QVMLRLIRQGVISKEDALQWSNKPDELRRQL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=39585 NITSA_RS08350 WP_013554587.1 1647606..1648661(+) (pilT) [Nitratifractor salsuginis DSM 16511]
ATGGCCTTCAATATTAAAAAACTGCTCCAAAGCGTCGTCGTCCACAAAGCCTCCGACCTCCACCTCATCGCCAAAAAAGA
GCCCCTGCTCCGGGTCGACGGAAAGCTCCGCCCCATCAATATGCCCAAACTCACCGGTGAGGATATCGAGGAGATGTGCT
ACGGTCTGCTGACCGAGAAGCAAAAGAAACATTTCGAAGAGGAGAATGAACTCGACTTCGCCGTGGAGTTGGAAGGGGTC
GGGCGTTTCCGGGTCAACTACTACCGCACCCTCGGAGATATGGCGGCGGCCTTCCGGATCATCCCCCTCTCCGTACCGAC
ACTGGACGAGATCGACGCTCCCCCGGTCTATCGAAAGATCATCCGGAGGGAAAAGGGGCTCATTCTCGTCACCGGCCCCA
CCGGCTCGGGTAAATCGACCACCCTGGCCGCCATGCTCAACGAGATCAACCTCACCGAGTCCAAACACATCATCACCGTG
GAAGACCCCGTGGAATTCGTCCACCAGCATAAAAAGTGCGTCTTTTCCCACCGAAGCGTCGGCGAAGACACCAAGAGCTT
CGCCGCGGCATTGAAATACGCCATGCGGGAAGACCCCGACATCATCCTTATCGGGGAGATGCGGGACAAGGAGACCATCG
CCGCGGCCCTGACCGCCGCCGAAACGGGTCACCTGGTCTTCGCCACGCTGCACACCAACTCCGCTCCCGGGACCGTCAAC
CGGATCATCGACGTCTTCAGCGGAGATGAGCAACCCCAGATCCGGGCTATGCTCTCCAGCTCCCTGGTCGCGGTCATCTC
CCAGGCCCTGCTTCCCAAGATCGGCGGCGGCCGGGTGGCCGTGCCCGAGATCCTCATCACCAACCACGCCATCGCCAACC
TGATCCGTGAAGACAAGGTCCACCAGATCTACTCCGCCATGCAGCTGGGCCAGGAGGATAGCGGGATGCAGACCCAGACC
CAGGTGATGCTCCGCCTGATCCGGCAAGGGGTCATCAGCAAAGAGGATGCCCTGCAGTGGTCTAACAAGCCCGATGAACT
CCGCCGGCAACTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

52.174

98.291

0.513
  pilT Acinetobacter baumannii D1279779

52.174

98.291

0.513
  pilT Acinetobacter nosocomialis M2

52.174

98.291

0.513
  pilT Neisseria gonorrhoeae MS11

52.035

98.006

0.51
  pilT Pseudomonas aeruginosa PAK

51.884

98.291

0.51
  pilT Pseudomonas stutzeri DSM 10701

51.744

98.006

0.507
  pilT Neisseria meningitidis 8013

51.744

98.006

0.507
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.153

94.872

0.504
  pilT Vibrio cholerae strain A1552

53.153

94.872

0.504
  pilT Acinetobacter baylyi ADP1

51.304

98.291

0.504
  pilT Legionella pneumophila strain ERS1305867

52.395

95.157

0.499
  pilT Legionella pneumophila strain Lp02

52.395

95.157

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.986

99.43

0.447
  pilU Pseudomonas stutzeri DSM 10701

42.651

98.86

0.422
  pilU Acinetobacter baylyi ADP1

41.076

100

0.413
  pilU Vibrio cholerae strain A1552

40.23

99.145

0.399

Multiple sequence alignment