Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU87_RS15050 Genome accession   NZ_CP045403
Coordinates   2872170..2873216 (-) Length   348 a.a.
NCBI ID   WP_152445345.1    Uniprot ID   A0A5P9HPX6
Organism   Bacillus sp. THAF10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2867170..2878216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU87_RS15020 (FIU87_15435) - 2867327..2868040 (-) 714 WP_152445339.1 pilus assembly protein PilO -
  FIU87_RS15025 (FIU87_15440) - 2868037..2868630 (-) 594 WP_152445340.1 PilN domain-containing protein -
  FIU87_RS15030 (FIU87_15445) pilM 2868630..2869613 (-) 984 WP_152445341.1 type IV pilus biogenesis protein PilM -
  FIU87_RS15035 (FIU87_15450) - 2869642..2870385 (-) 744 WP_152445342.1 A24 family peptidase -
  FIU87_RS15040 (FIU87_15455) - 2870393..2870875 (-) 483 WP_152445343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU87_RS15045 (FIU87_15460) - 2870969..2872177 (-) 1209 WP_152445344.1 type II secretion system F family protein -
  FIU87_RS15050 (FIU87_15465) pilT 2872170..2873216 (-) 1047 WP_152445345.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU87_RS15055 (FIU87_15470) - 2873225..2874892 (-) 1668 WP_152445346.1 GspE/PulE family protein -
  FIU87_RS15060 (FIU87_15475) - 2874916..2876301 (-) 1386 WP_216647485.1 hypothetical protein -
  FIU87_RS15065 - 2876419..2876694 (-) 276 WP_152445348.1 hypothetical protein -
  FIU87_RS15070 (FIU87_15480) - 2876796..2877482 (+) 687 WP_152445349.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FIU87_RS15075 (FIU87_15485) - 2877466..2878110 (+) 645 WP_152445350.1 type II secretion system protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38823.70 Da        Isoelectric Point: 6.8387

>NTDB_id=393813 FIU87_RS15050 WP_152445345.1 2872170..2873216(-) (pilT) [Bacillus sp. THAF10]
MIEKMKSWLHTAYDSKASDIHLSVGVPPVFRINGELTRHGEDSLTPEDMEILAKEMIPDFMWERFEEKGELDFSYSVPGI
SRFRVNAYRQRTSVAIAIRIIPTRIPSLEELQLPYILQSLVAKPQGLFLVTGPTGSGKSTTLASMIDYLNRMEKKHIITL
EDPIEYLHKHNKCIIDQREVGFDTKNFASGLRASLRQDPDIILVGELRDLETIQTAITAAETGHLVLGTLHTTSAPATID
RIIDVFPGGQQAQIRVQLASVLVSIVSQRLFPTADRSGRKVATEILVNTPAVANLIRNEKIHQIQSVMQTSRALGMHTLE
MSIRQLIQNGVISKASAESFLQEAVNYA

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=393813 FIU87_RS15050 WP_152445345.1 2872170..2873216(-) (pilT) [Bacillus sp. THAF10]
ATGATTGAGAAAATGAAAAGCTGGCTGCATACTGCCTATGATTCCAAGGCGTCAGACATTCACCTGTCCGTAGGTGTTCC
CCCAGTTTTTCGGATCAATGGGGAGCTTACTCGTCACGGAGAGGATAGTCTAACACCAGAGGACATGGAAATACTAGCAA
AGGAAATGATTCCAGATTTTATGTGGGAGCGCTTTGAGGAAAAAGGGGAATTGGACTTCTCTTACAGTGTTCCTGGAATT
TCCCGTTTTCGGGTAAATGCGTACCGCCAACGAACGTCTGTGGCCATTGCCATCCGAATTATTCCAACAAGAATTCCATC
TCTTGAGGAGCTTCAGCTGCCATATATTCTGCAAAGCTTGGTAGCCAAACCACAAGGACTTTTTCTCGTAACTGGTCCTA
CTGGTAGTGGAAAATCAACCACACTCGCTTCAATGATTGATTACCTAAACAGAATGGAAAAAAAACATATCATCACACTT
GAGGATCCAATTGAATACCTCCACAAGCATAATAAGTGTATTATTGACCAGCGTGAGGTTGGATTTGATACGAAAAATTT
TGCATCAGGTCTACGTGCCTCCCTACGGCAAGACCCTGATATCATTCTTGTCGGAGAGCTTCGAGATCTTGAAACGATTC
AAACTGCCATTACTGCTGCAGAAACCGGTCATCTCGTACTAGGAACACTGCATACTACAAGTGCGCCTGCTACTATTGAC
AGAATAATTGACGTGTTTCCTGGAGGACAGCAGGCTCAAATTAGAGTGCAGCTAGCCTCGGTACTAGTATCAATTGTCTC
CCAGAGGCTGTTTCCAACCGCAGATAGAAGCGGGAGAAAGGTAGCAACCGAGATTCTTGTAAATACACCTGCAGTAGCAA
ACTTGATTCGAAACGAAAAAATCCATCAAATACAAAGCGTGATGCAAACAAGTCGGGCACTCGGCATGCACACCTTAGAA
ATGAGCATCAGGCAGCTAATTCAAAACGGAGTTATTTCAAAAGCTTCTGCCGAAAGCTTCTTACAGGAGGCTGTGAACTA
TGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9HPX6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.615

93.391

0.491
  pilT Vibrio cholerae strain A1552

50.459

93.966

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

93.966

0.474
  pilT Acinetobacter nosocomialis M2

49.096

95.402

0.468
  pilT Acinetobacter baumannii D1279779

49.096

95.402

0.468
  pilT Acinetobacter baumannii strain A118

49.096

95.402

0.468
  pilT Pseudomonas aeruginosa PAK

49.39

94.253

0.466
  pilT Acinetobacter baylyi ADP1

48.923

93.391

0.457
  pilT Pseudomonas stutzeri DSM 10701

47.041

97.126

0.457
  pilT Legionella pneumophila strain ERS1305867

48.297

92.816

0.448
  pilT Legionella pneumophila strain Lp02

48.297

92.816

0.448
  pilT Neisseria meningitidis 8013

48.276

91.667

0.443
  pilT Neisseria gonorrhoeae MS11

47.678

92.816

0.443
  pilU Pseudomonas stutzeri DSM 10701

41.124

97.126

0.399
  pilU Vibrio cholerae strain A1552

41.018

95.977

0.394
  pilU Acinetobacter baylyi ADP1

42.105

92.816

0.391

Multiple sequence alignment