Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIU98_RS22185 Genome accession   NZ_CP045359
Coordinates   4753477..4754511 (-) Length   344 a.a.
NCBI ID   WP_037056538.1    Uniprot ID   A0A5P9CD94
Organism   Pseudomonas sp. THAF42     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4748477..4759511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU98_RS22165 (FIU98_22060) - 4749643..4750041 (-) 399 WP_037056528.1 TM2 domain-containing protein -
  FIU98_RS22170 (FIU98_22065) - 4750189..4751400 (+) 1212 WP_037056531.1 putative DNA modification/repair radical SAM protein -
  FIU98_RS22175 (FIU98_22070) - 4751400..4752284 (+) 885 WP_152489805.1 TIGR03915 family putative DNA repair protein -
  FIU98_RS22180 (FIU98_22075) pilU 4752250..4753395 (-) 1146 WP_037056536.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU98_RS22185 (FIU98_22080) pilT 4753477..4754511 (-) 1035 WP_037056538.1 type IV pilus twitching motility protein PilT Machinery gene
  FIU98_RS22190 (FIU98_22085) - 4754727..4755419 (+) 693 WP_134676426.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIU98_RS22195 (FIU98_22090) proC 4755437..4756255 (+) 819 WP_152489806.1 pyrroline-5-carboxylate reductase -
  FIU98_RS22200 (FIU98_22095) - 4756265..4756858 (+) 594 WP_037056544.1 YggT family protein -
  FIU98_RS22205 (FIU98_22100) - 4756863..4757153 (+) 291 WP_037056545.1 DUF167 domain-containing protein -
  FIU98_RS22210 (FIU98_22105) - 4757363..4759330 (+) 1968 WP_152489807.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37933.62 Da        Isoelectric Point: 6.8889

>NTDB_id=393653 FIU98_RS22185 WP_037056538.1 4753477..4754511(-) (pilT) [Pseudomonas sp. THAF42]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRDSAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=393653 FIU98_RS22185 WP_037056538.1 4753477..4754511(-) (pilT) [Pseudomonas sp. THAF42]
ATGGATATCACTGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGATTTGCACCTCTCGGCCGGTCTGCCGCCGAT
GATCCGCGTAGACGGCGATGTTCGCCGCATCAACCTGCCGCCGCTGGATCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGATTTCGAAGAGTTCCTCGAGACCGACTTCTCCTTCGAAGTACCTGGCGTCGCGCGT
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCAAGGTCCTGACCATGGA
AGACCTCGGCATGGGCGACGTGTTCCGCAAGATCACCGACGTGCCGCGCGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTCGACTACCTCAACAGCAACAAGTATCACCACATCCTCACCATCGAAGAC
CCGATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGATACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATCCTGGTCGGTGAGATGCGTGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTCTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCGATGGTTCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGATCGGCGGTGGTCGTGTGGCCGCTCACGAAATCATGATCGGTACGCCGGCCATCCGTAACCTGATCC
GTGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGTGGTGCGCTGGGCATGCAGACCCTCGATGCCTGCCTC
AAGACCCTGGTCAGCAAGGGGTTGGTCAGCCGCGACAGTGCGCGGGAAAAGGCCAAGACCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9CD94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

95.058

100

0.951
  pilT Pseudomonas aeruginosa PAK

93.895

100

0.939
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

81.686

100

0.817
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

97.965

0.674
  pilT Vibrio cholerae strain A1552

68.843

97.965

0.674
  pilT Neisseria meningitidis 8013

66.667

100

0.669
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment