Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIV08_RS02160 Genome accession   NZ_CP045324
Coordinates   478225..479259 (+) Length   344 a.a.
NCBI ID   WP_058091364.1    Uniprot ID   -
Organism   Marinobacter sp. THAF197a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 419518..486352 478225..479259 within 0


Gene organization within MGE regions


Location: 419518..486352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV08_RS01890 (FIV08_01920) - 419620..420459 (-) 840 WP_152437179.1 uroporphyrinogen-III synthase -
  FIV08_RS01895 (FIV08_01925) hemC 420440..421381 (-) 942 WP_152437180.1 hydroxymethylbilane synthase -
  FIV08_RS01900 (FIV08_01930) - 421467..422222 (-) 756 WP_061332519.1 LytR/AlgR family response regulator transcription factor -
  FIV08_RS01905 (FIV08_01935) - 422253..423341 (-) 1089 WP_416376904.1 sensor histidine kinase -
  FIV08_RS01910 (FIV08_01940) argH 423401..424816 (+) 1416 WP_152437182.1 argininosuccinate lyase -
  FIV08_RS19785 - 424959..426053 (+) 1095 WP_228715476.1 hypothetical protein -
  FIV08_RS19790 - 426139..427041 (+) 903 WP_267313115.1 phospholipase effector Tle1 domain-containing protein -
  FIV08_RS01920 (FIV08_01955) - 427038..427643 (+) 606 WP_323847459.1 DUF2931 family protein -
  FIV08_RS01925 (FIV08_01960) - 427678..428283 (+) 606 WP_323847460.1 DUF2931 family protein -
  FIV08_RS01930 (FIV08_01965) - 428321..428923 (+) 603 WP_152437183.1 DUF2931 family protein -
  FIV08_RS01935 (FIV08_01970) - 428942..431383 (-) 2442 WP_152437184.1 EAL domain-containing protein -
  FIV08_RS01940 (FIV08_01975) - 431523..434372 (+) 2850 WP_152437185.1 class I adenylate cyclase -
  FIV08_RS01945 (FIV08_01980) lptM 434445..434615 (+) 171 WP_228715477.1 LPS translocon maturation chaperone LptM -
  FIV08_RS01950 (FIV08_01985) lysA 434660..435910 (+) 1251 WP_061332524.1 diaminopimelate decarboxylase -
  FIV08_RS01955 (FIV08_01990) dapF 435925..436839 (+) 915 WP_152437187.1 diaminopimelate epimerase -
  FIV08_RS01960 (FIV08_01995) - 436857..437609 (+) 753 WP_061332525.1 DUF484 family protein -
  FIV08_RS01965 (FIV08_02000) xerC 437645..438577 (+) 933 WP_152439571.1 tyrosine recombinase XerC -
  FIV08_RS01970 (FIV08_02005) - 438589..439983 (+) 1395 WP_152437188.1 GGDEF domain-containing protein -
  FIV08_RS01975 (FIV08_02010) - 439993..440226 (-) 234 WP_061332527.1 DUF2789 domain-containing protein -
  FIV08_RS01980 (FIV08_02015) - 440324..443080 (+) 2757 WP_152437189.1 ATP-binding protein -
  FIV08_RS01985 (FIV08_02020) - 443094..443459 (-) 366 WP_072678634.1 hypothetical protein -
  FIV08_RS01990 (FIV08_02025) - 443691..444299 (+) 609 WP_152437190.1 hypothetical protein -
  FIV08_RS01995 (FIV08_02030) - 444307..446517 (+) 2211 WP_228715478.1 patatin-like phospholipase family protein -
  FIV08_RS02000 (FIV08_02035) - 446538..447200 (-) 663 WP_152437192.1 GntR family transcriptional regulator -
  FIV08_RS02005 (FIV08_02040) cls 447278..448714 (-) 1437 WP_152437193.1 cardiolipin synthase -
  FIV08_RS02010 (FIV08_02045) - 448711..449751 (-) 1041 WP_058091334.1 AraC family transcriptional regulator -
  FIV08_RS02015 (FIV08_02055) - 450139..450930 (+) 792 WP_152437194.1 alpha/beta fold hydrolase -
  FIV08_RS02020 (FIV08_02060) - 451033..452118 (+) 1086 WP_152439572.1 SMP-30/gluconolactonase/LRE family protein -
  FIV08_RS02025 (FIV08_02065) - 452115..452579 (+) 465 WP_058091336.1 acyl-CoA thioesterase -
  FIV08_RS02030 (FIV08_02070) - 452657..453637 (+) 981 WP_152437195.1 NADP-dependent oxidoreductase -
  FIV08_RS02035 (FIV08_02075) tcdA 453634..454431 (-) 798 WP_061332537.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
  FIV08_RS02040 (FIV08_02080) - 454690..456465 (-) 1776 WP_072678623.1 acyl-CoA dehydrogenase C-terminal domain-containing protein -
  FIV08_RS02045 (FIV08_02085) - 456642..457376 (+) 735 WP_152437196.1 SDR family oxidoreductase -
  FIV08_RS02050 (FIV08_02090) - 457395..458339 (-) 945 WP_072678622.1 sodium-dependent bicarbonate transport family permease -
  FIV08_RS02055 (FIV08_02095) - 458514..459152 (-) 639 WP_152437197.1 ChrR family anti-sigma-E factor -
  FIV08_RS02060 (FIV08_02100) - 459149..459748 (-) 600 WP_152437198.1 sigma-70 family RNA polymerase sigma factor -
  FIV08_RS02065 (FIV08_02105) - 459977..461023 (+) 1047 WP_152437199.1 AraC family transcriptional regulator -
  FIV08_RS02070 (FIV08_02110) speA 461027..462937 (-) 1911 WP_152437200.1 biosynthetic arginine decarboxylase -
  FIV08_RS02075 (FIV08_02115) speE 463167..464048 (+) 882 WP_061332546.1 polyamine aminopropyltransferase -
  FIV08_RS02080 (FIV08_02120) - 464141..464335 (+) 195 WP_061332547.1 DUF6316 family protein -
  FIV08_RS02085 (FIV08_02125) - 464412..465659 (-) 1248 WP_152437201.1 ammonium transporter -
  FIV08_RS02090 (FIV08_02130) glnK 465731..466069 (-) 339 WP_058091350.1 P-II family nitrogen regulator -
  FIV08_RS02095 (FIV08_02135) - 466454..466705 (+) 252 WP_061332549.1 accessory factor UbiK family protein -
  FIV08_RS02100 (FIV08_02140) - 466779..468305 (+) 1527 WP_152437202.1 YifB family Mg chelatase-like AAA ATPase -
  FIV08_RS02105 (FIV08_02145) trmB 468378..469103 (+) 726 WP_061332551.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  FIV08_RS02110 (FIV08_02150) - 469116..469664 (-) 549 WP_061332552.1 hypothetical protein -
  FIV08_RS02115 (FIV08_02155) hemW 469664..470809 (-) 1146 WP_152437203.1 radical SAM family heme chaperone HemW -
  FIV08_RS02120 (FIV08_02160) rdgB 470814..471413 (-) 600 WP_061332554.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  FIV08_RS02125 (FIV08_02165) - 471418..471864 (-) 447 WP_152439573.1 DUF4426 domain-containing protein -
  FIV08_RS02130 (FIV08_02170) metW 471864..472466 (-) 603 WP_061332555.1 methionine biosynthesis protein MetW -
  FIV08_RS02135 (FIV08_02175) metX 472463..473611 (-) 1149 WP_061332556.1 homoserine O-succinyltransferase MetX -
  FIV08_RS02140 (FIV08_02180) - 473784..475748 (-) 1965 WP_152437204.1 dynamin family protein -
  FIV08_RS02145 (FIV08_02185) - 475955..476539 (-) 585 WP_061332558.1 YggT family protein -
  FIV08_RS02150 (FIV08_02190) proC 476562..477395 (-) 834 WP_061332559.1 pyrroline-5-carboxylate reductase -
  FIV08_RS02155 (FIV08_02195) - 477459..478172 (-) 714 WP_152437205.1 YggS family pyridoxal phosphate-dependent enzyme -
  FIV08_RS02160 (FIV08_02200) pilT 478225..479259 (+) 1035 WP_058091364.1 type IV pilus twitching motility protein PilT Machinery gene
  FIV08_RS02165 (FIV08_02205) pilU 479483..480604 (+) 1122 WP_061332594.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIV08_RS02170 (FIV08_02210) - 480763..482136 (+) 1374 WP_152437206.1 OmpP1/FadL family transporter -
  FIV08_RS02175 (FIV08_02215) - 482176..482529 (-) 354 WP_152437207.1 hypothetical protein -
  FIV08_RS02180 (FIV08_02220) polA 482727..485459 (-) 2733 WP_152437208.1 DNA polymerase I -
  FIV08_RS02185 (FIV08_02225) - 485545..485880 (+) 336 WP_061332562.1 DUF2782 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38146.88 Da        Isoelectric Point: 6.2443

>NTDB_id=393435 FIV08_RS02160 WP_058091364.1 478225..479259(+) (pilT) [Marinobacter sp. THAF197a]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKEVHGLIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGQVFKDISSVPRGLVLVTGPTGSGKSTTLAAMMDYINDTRYEHILTIED
PIEFVHDSKKCLVNQREVHRDTLGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLMKKMGGGRIAAHEIMIGTSAIRNLIREDKIAQMYSSIQTGGSLGMQTLDQCL
ERLLQKGLISREAARAKAKMPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=393435 FIV08_RS02160 WP_058091364.1 478225..479259(+) (pilT) [Marinobacter sp. THAF197a]
ATGGATATTACTGAACTGCTTGCCTTTTCGGCGAAACAGGGCGCATCGGATTTGCACCTGTCTGCCGGTCTGCCCCCCAT
GATCCGTGTCGACGGCGACGTGCGCCGCATCAACCTGCCCCCGATGGAGCACAAAGAAGTGCACGGGTTGATCTACGACA
TCATGAACGACAAGCAGCGTAAGGATTACGAAGAGTTCCTGGAAACCGACTTCTCGTTCGAGGTGCCGGGTGTGGCCCGT
TTCCGGGTAAACGCCTTCAACCAGAACCGTGGCGCCGGCGCTGTATTCCGGACCATCCCCTCGAAGGTTCTGACCATGGA
AGATCTGGGCATGGGGCAGGTGTTCAAGGATATTTCGTCCGTACCCCGTGGCCTGGTGCTGGTCACCGGGCCCACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCGATGATGGACTACATCAACGACACCCGCTATGAGCATATCCTGACCATCGAAGAC
CCGATCGAATTCGTGCACGACTCGAAAAAGTGCCTGGTCAACCAGCGGGAAGTGCACCGGGATACCCTGGGCTTTAACGA
AGCCCTGCGTTCCGCCTTGCGGGAAGACCCGGACATCATCCTGGTAGGTGAGCTCCGGGACCTGGAAACCATCCGACTGG
CCCTGACCGCCGCAGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCTGCGAAGACCATCGACCGGGTG
GTTGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTGCGGTCCATGTTGTCGGAATCCCTGCAGGCGGTTATCTCCCAGAC
CCTGATGAAGAAGATGGGCGGCGGCCGGATTGCCGCCCACGAGATCATGATCGGTACGTCTGCGATCCGTAACCTGATCC
GGGAAGACAAGATTGCGCAGATGTACTCATCCATCCAGACCGGTGGCTCGCTGGGCATGCAGACCCTCGACCAGTGCCTG
GAGCGCCTGCTGCAGAAGGGTCTGATTTCCCGCGAAGCAGCGCGGGCCAAGGCCAAGATGCCTGATAACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

90.116

100

0.901
  pilT Pseudomonas stutzeri DSM 10701

90.116

100

0.901
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baumannii D1279779

83.43

100

0.834
  pilT Acinetobacter baumannii strain A118

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

77.219

98.256

0.759
  pilT Legionella pneumophila strain ERS1305867

77.219

98.256

0.759
  pilT Neisseria gonorrhoeae MS11

68.235

98.837

0.674
  pilT Neisseria meningitidis 8013

68.235

98.837

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.471

98.837

0.657
  pilT Vibrio cholerae strain A1552

66.471

98.837

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.384

0.401
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392
  pilU Vibrio cholerae strain A1552

39.701

97.384

0.387

Multiple sequence alignment