Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SELIN_RS11445 Genome accession   NC_014836
Coordinates   2447283..2448347 (+) Length   354 a.a.
NCBI ID   WP_013506813.1    Uniprot ID   E6W3Q6
Organism   Desulfurispirillum indicum S5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2442283..2453347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SELIN_RS11425 (Selin_2213) - 2442625..2443275 (-) 651 WP_013506809.1 TetR/AcrR family transcriptional regulator -
  SELIN_RS11430 (Selin_2214) - 2443527..2445413 (+) 1887 WP_013506810.1 N-acetylmuramoyl-L-alanine amidase -
  SELIN_RS11435 (Selin_2215) - 2445425..2445817 (+) 393 WP_013506811.1 hypothetical protein -
  SELIN_RS11440 (Selin_2216) - 2445828..2447240 (+) 1413 WP_013506812.1 phosphomannomutase/phosphoglucomutase -
  SELIN_RS11445 (Selin_2217) pilT 2447283..2448347 (+) 1065 WP_013506813.1 type IV pilus twitching motility protein PilT Machinery gene
  SELIN_RS11450 (Selin_2218) topA 2448621..2450927 (+) 2307 WP_013506814.1 type I DNA topoisomerase -
  SELIN_RS11455 (Selin_2219) - 2451001..2451459 (-) 459 WP_013506815.1 hypothetical protein -
  SELIN_RS11460 (Selin_2220) panD 2451531..2451923 (-) 393 WP_013506816.1 aspartate 1-decarboxylase -
  SELIN_RS11465 (Selin_2221) panC 2451929..2452792 (-) 864 WP_041726094.1 pantoate--beta-alanine ligase -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38818.64 Da        Isoelectric Point: 6.5920

>NTDB_id=39291 SELIN_RS11445 WP_013506813.1 2447283..2448347(+) (pilT) [Desulfurispirillum indicum S5]
MNDTLKGLLQIMADQGASDLHLVAGSPAQIRVDGRLRPVNQTVLSGEDIDLMCRSTVEGKEIARFQESGELDKALFLPGI
ARFRLNLYKALGETAAAFRIIPEKIPALDEIGCPPMYKQLVKREKGLVLVTGPTGSGKSTTLASMLNEINLTEHKHIITI
EDPVEFIHRHDKSLFSHRNIGRDTQSFPQALKYALREDPDVILIGEMRDRATIEAALTAAETGHLVFGTLHTNSASQTIN
RIIDVFPGDEQSQIRAQLSVSLVAVIAQVLVPRIGGGRVAVPEILLNTSAVGNLIRESKVHQIYSQMDLGRNLGMQTQVR
MMAQLVQDGVVERTTAFQFSNNPEELMRVLGTGS

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=39291 SELIN_RS11445 WP_013506813.1 2447283..2448347(+) (pilT) [Desulfurispirillum indicum S5]
ATGAATGATACCCTGAAGGGCCTTTTGCAGATCATGGCTGACCAAGGTGCGTCGGATTTGCACCTGGTTGCCGGCAGTCC
GGCGCAGATTCGCGTTGATGGTCGTTTACGGCCGGTTAACCAGACCGTGCTCAGTGGTGAAGACATTGATCTGATGTGCC
GCAGTACCGTTGAGGGCAAAGAAATTGCCCGCTTCCAGGAGAGTGGCGAGCTGGACAAGGCGCTTTTTCTGCCGGGTATC
GCTCGTTTTCGCCTGAATCTGTATAAGGCGCTGGGGGAGACGGCGGCGGCATTCCGCATTATTCCCGAGAAGATTCCCGC
CCTGGACGAGATCGGTTGCCCCCCCATGTATAAGCAGCTGGTCAAACGCGAGAAGGGGCTTGTCCTGGTGACGGGGCCGA
CGGGCAGCGGCAAGTCGACCACCCTGGCGTCCATGCTCAATGAGATCAATCTGACAGAGCATAAACACATTATTACCATT
GAAGATCCGGTGGAGTTTATTCACCGGCACGACAAGTCACTCTTCTCTCACCGCAATATCGGCCGGGATACCCAGAGCTT
CCCCCAGGCCCTCAAGTACGCCCTGCGGGAAGATCCCGATGTGATCCTGATCGGCGAAATGCGTGATCGCGCCACCATTG
AAGCGGCGTTGACCGCCGCGGAGACCGGACACCTGGTATTCGGCACCCTGCACACCAATTCGGCCTCCCAGACCATCAAC
CGCATCATTGACGTCTTCCCCGGTGACGAGCAGTCGCAGATACGGGCTCAGCTTTCCGTCAGTCTGGTGGCAGTCATCGC
CCAGGTGCTGGTGCCCCGGATCGGTGGCGGGCGGGTGGCTGTGCCGGAGATTCTCCTCAACACCAGTGCTGTGGGCAACC
TGATCCGTGAGAGCAAGGTGCATCAGATTTACTCCCAGATGGATCTCGGCCGCAACCTGGGGATGCAGACCCAGGTGCGG
ATGATGGCGCAGCTGGTGCAGGATGGTGTGGTGGAGCGTACCACGGCGTTTCAGTTCAGCAACAACCCCGAAGAGCTGAT
GCGTGTTCTGGGGACAGGGAGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6W3Q6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.147

95.763

0.48
  pilT Pseudomonas stutzeri DSM 10701

48.968

95.763

0.469
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.077

91.808

0.469
  pilT Vibrio cholerae strain A1552

51.077

91.808

0.469
  pilT Acinetobacter baumannii strain A118

48.529

96.045

0.466
  pilT Acinetobacter nosocomialis M2

48.529

96.045

0.466
  pilT Acinetobacter baumannii D1279779

48.529

96.045

0.466
  pilT Neisseria gonorrhoeae MS11

47.941

96.045

0.46
  pilT Neisseria meningitidis 8013

47.941

96.045

0.46
  pilT Acinetobacter baylyi ADP1

47.493

95.763

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

98.305

0.455
  pilT Legionella pneumophila strain ERS1305867

47.761

94.633

0.452
  pilT Legionella pneumophila strain Lp02

47.761

94.633

0.452
  pilU Pseudomonas stutzeri DSM 10701

40.563

100

0.407
  pilU Acinetobacter baylyi ADP1

39.535

97.175

0.384
  pilU Vibrio cholerae strain A1552

37.677

99.718

0.376

Multiple sequence alignment