Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BCELL_RS15300 Genome accession   NC_014829
Coordinates   3294816..3295850 (-) Length   344 a.a.
NCBI ID   WP_013489676.1    Uniprot ID   E6TZN9
Organism   Evansella cellulosilytica DSM 2522     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3289816..3300850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCELL_RS15270 (Bcell_3096) pilO 3289861..3290550 (-) 690 WP_013489670.1 type 4a pilus biogenesis protein PilO -
  BCELL_RS15275 (Bcell_3097) - 3290547..3291140 (-) 594 WP_013489671.1 PilN domain-containing protein -
  BCELL_RS15280 (Bcell_3098) pilM 3291144..3292115 (-) 972 WP_013489672.1 pilus assembly protein PilM -
  BCELL_RS15285 (Bcell_3099) - 3292133..3292885 (-) 753 WP_013489673.1 A24 family peptidase -
  BCELL_RS15290 (Bcell_3100) - 3292924..3293346 (-) 423 WP_013489674.1 type II secretion system protein -
  BCELL_RS15295 (Bcell_3101) - 3293605..3294819 (-) 1215 WP_342633086.1 type II secretion system F family protein -
  BCELL_RS15300 (Bcell_3102) pilT 3294816..3295850 (-) 1035 WP_013489676.1 type IV pilus twitching motility protein PilT Machinery gene
  BCELL_RS15305 (Bcell_3103) - 3295861..3297522 (-) 1662 WP_013489677.1 GspE/PulE family protein -
  BCELL_RS21735 (Bcell_3104) - 3297821..3298249 (+) 429 WP_013489678.1 hypothetical protein -
  BCELL_RS15315 (Bcell_3105) - 3298260..3298733 (+) 474 WP_013489679.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BCELL_RS15320 (Bcell_3106) - 3298730..3300184 (+) 1455 WP_013489680.1 pilus assembly PilX N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38561.46 Da        Isoelectric Point: 6.9402

>NTDB_id=39240 BCELL_RS15300 WP_013489676.1 3294816..3295850(-) (pilT) [Evansella cellulosilytica DSM 2522]
MGRSLKKLLSEAIYLEASDIHLTIGKPPVLRIHGELITLDQESLKPEDIKEMVEEVLPEALKEQLETNREVDFSYGIPGV
SRFRLNAYYQRSHLSMAIRVIQVQIPSLEDLAMPSVLKTIAEKKQGLVLVTGPTGSGKSTTLAAMIKHMNENVKKHIITL
EDPIEYLHKHRNCIIDQREVGSDTKSFSTGLRSALRQDPDVILVGEMRDLDTIATAITAAETGHLVLGTLHTSSAAATID
RIIDVFQPSQQAQIRIQLASVLAAVISQRLLTTVDRRGRKAATEILVNNYAIANLIRNEKIHQIENVLQTSRSEGMHTLE
MSIRQLLTDRLIDYEEAKHYIKER

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=39240 BCELL_RS15300 WP_013489676.1 3294816..3295850(-) (pilT) [Evansella cellulosilytica DSM 2522]
GTGGGTCGAAGCTTAAAAAAATTATTATCAGAAGCTATTTATCTCGAAGCCTCGGATATACACTTAACGATTGGGAAACC
GCCAGTACTAAGGATTCATGGTGAATTAATAACACTCGATCAGGAAAGTTTAAAACCTGAGGACATTAAAGAGATGGTAG
AAGAGGTATTACCAGAAGCGTTAAAGGAACAGCTAGAAACAAATCGTGAAGTCGACTTTTCATATGGAATTCCAGGTGTT
TCGCGTTTTCGTTTAAATGCATATTACCAAAGGTCGCACTTAAGCATGGCGATTCGTGTCATTCAAGTGCAAATCCCGAG
TTTAGAAGACTTAGCGATGCCGAGTGTGTTAAAGACAATTGCAGAGAAAAAGCAAGGGTTAGTGTTAGTGACAGGTCCTA
CTGGTAGCGGGAAATCGACGACTTTAGCCGCTATGATTAAGCACATGAACGAAAATGTAAAGAAGCACATTATTACGCTC
GAAGACCCAATCGAGTATTTGCACAAGCATAGAAACTGTATTATTGATCAAAGAGAGGTTGGATCTGATACGAAGAGCTT
CTCTACAGGATTGAGGTCGGCACTTCGACAAGATCCAGACGTTATTTTAGTAGGGGAAATGCGAGATTTAGACACGATTG
CTACAGCCATTACAGCAGCAGAAACGGGTCATCTCGTTTTAGGCACGCTACACACATCTAGCGCTGCTGCAACAATTGAT
CGTATTATCGATGTATTCCAGCCAAGTCAGCAAGCACAAATTAGAATTCAGCTCGCTTCTGTTTTAGCAGCTGTCATTTC
CCAACGTTTATTAACGACGGTCGATCGTCGTGGACGAAAAGCGGCAACAGAAATACTTGTGAACAATTATGCGATAGCGA
ATTTAATTAGAAACGAAAAAATACATCAAATCGAAAATGTACTTCAAACGAGTCGTTCAGAAGGAATGCATACGCTAGAA
ATGAGCATAAGACAGCTTTTAACGGATCGCTTAATCGATTATGAAGAGGCAAAACATTATATAAAAGAGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6TZN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.112

98.256

0.483
  pilT Vibrio cholerae strain A1552

49.112

98.256

0.483
  pilT Neisseria meningitidis 8013

49.245

96.221

0.474
  pilT Neisseria gonorrhoeae MS11

48.943

96.221

0.471
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.221

93.314

0.459
  pilT Acinetobacter baumannii strain A118

46.313

98.547

0.456
  pilT Acinetobacter nosocomialis M2

46.313

98.547

0.456
  pilT Pseudomonas stutzeri DSM 10701

46.313

98.547

0.456
  pilT Acinetobacter baumannii D1279779

46.313

98.547

0.456
  pilT Pseudomonas aeruginosa PAK

45.858

98.256

0.451
  pilT Acinetobacter baylyi ADP1

45.562

98.256

0.448
  pilT Legionella pneumophila strain ERS1305867

45.427

95.349

0.433
  pilT Legionella pneumophila strain Lp02

45.427

95.349

0.433
  pilU Pseudomonas stutzeri DSM 10701

42.042

96.802

0.407
  pilU Acinetobacter baylyi ADP1

40.798

94.767

0.387
  pilU Vibrio cholerae strain A1552

39.104

97.384

0.381

Multiple sequence alignment