Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ETHHA_RS04030 Genome accession   NC_014828
Coordinates   867194..868255 (+) Length   353 a.a.
NCBI ID   WP_013484731.1    Uniprot ID   E6U2Z4
Organism   Ethanoligenens harbinense YUAN-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862194..873255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETHHA_RS04010 (Ethha_0788) - 862326..862895 (+) 570 WP_013484727.1 ferritin family protein -
  ETHHA_RS04015 (Ethha_0789) ugpC 863040..864176 (-) 1137 WP_013484728.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
  ETHHA_RS04020 (Ethha_0790) - 864608..865366 (+) 759 WP_013484729.1 A24 family peptidase -
  ETHHA_RS04025 (Ethha_0791) - 865414..867090 (+) 1677 WP_106919267.1 GspE/PulE family protein -
  ETHHA_RS04030 (Ethha_0792) pilT 867194..868255 (+) 1062 WP_013484731.1 type IV pilus twitching motility protein PilT Machinery gene
  ETHHA_RS14395 (Ethha_0793) - 868370..868810 (+) 441 WP_013484732.1 type II secretion system protein -
  ETHHA_RS04040 (Ethha_0794) - 868932..869345 (+) 414 WP_013484733.1 type II secretion system protein -
  ETHHA_RS04045 (Ethha_0795) - 869401..870171 (-) 771 WP_013484734.1 sigma-70 family RNA polymerase sigma factor -
  ETHHA_RS04050 (Ethha_0796) - 870205..870795 (-) 591 WP_013484735.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ETHHA_RS04055 (Ethha_0797) - 870814..871191 (-) 378 WP_013484736.1 type II secretion system protein -
  ETHHA_RS04060 (Ethha_0798) - 871188..872816 (-) 1629 WP_013484737.1 polymer-forming cytoskeletal protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39391.49 Da        Isoelectric Point: 6.5866

>NTDB_id=39208 ETHHA_RS04030 WP_013484731.1 867194..868255(+) (pilT) [Ethanoligenens harbinense YUAN-3]
MKRVDELLFKAMDCKSSDLHLTVGVPPMVRINGLLRPLEGYDVLLPEDTQEIAASMLTEAQNQEVETKGQTDFSYVIQDV
GRFRVNIYKQRRSYSIAIRVLAATMPTIESLGLPGTLKELALRPRGLVLVTGPTGSGKSTTLAAMVDYINMNRRCHVLTI
EDPIEYLHKHKNSIVNQREVGDDTDSFAGALRSALREDPDVILVGEMRDLETISTAISAAETGHFVLSTLHTNGAAQTVD
RIIDMFPPFQQQQIRVQLSSVLQAVITQQLLPNVLHDGRVVAMELMLCNDAVRNMIRDNKCHQIVTALQTGIKQGMMPMD
YSLSRLVKANQITQAEAMSHSMDKETLRRYLTM

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=39208 ETHHA_RS04030 WP_013484731.1 867194..868255(+) (pilT) [Ethanoligenens harbinense YUAN-3]
ATGAAACGGGTCGATGAATTGCTTTTTAAAGCGATGGACTGCAAAAGCTCCGATCTGCACCTCACGGTGGGGGTGCCTCC
CATGGTGCGGATCAACGGCCTGCTCCGGCCACTGGAAGGCTATGATGTTCTGCTGCCCGAAGACACGCAGGAAATTGCCG
CTTCCATGCTTACGGAAGCGCAAAACCAAGAGGTGGAAACAAAAGGCCAAACGGATTTTTCGTATGTGATACAGGATGTC
GGACGTTTCCGTGTCAATATCTACAAGCAGCGGCGCAGCTATTCCATCGCTATCCGCGTGCTGGCGGCAACGATGCCGAC
CATTGAAAGCCTGGGCCTGCCGGGCACGCTCAAGGAGCTGGCGCTCAGGCCGCGCGGCCTTGTGCTCGTTACCGGGCCGA
CGGGCAGCGGCAAGTCCACCACGCTGGCGGCCATGGTGGATTATATCAATATGAACCGGCGCTGCCATGTGCTGACCATC
GAAGACCCGATCGAATACCTGCACAAGCACAAGAACAGCATCGTCAACCAGCGCGAGGTCGGCGACGATACCGATTCTTT
TGCCGGGGCGCTGCGCTCCGCTTTGCGTGAGGACCCGGATGTCATTCTCGTGGGCGAGATGCGCGATCTGGAGACCATCT
CCACTGCCATCTCCGCTGCCGAAACCGGCCACTTCGTGCTTTCCACGCTGCACACCAACGGCGCCGCGCAGACGGTCGAC
CGCATCATCGATATGTTTCCGCCCTTCCAGCAGCAACAGATACGCGTACAGCTTTCCTCGGTGCTGCAGGCGGTCATCAC
GCAGCAACTGCTGCCCAACGTGCTGCACGACGGGCGGGTGGTCGCCATGGAGCTGATGCTCTGCAACGACGCTGTGCGCA
ACATGATACGCGACAACAAGTGCCACCAGATCGTTACCGCGTTGCAGACCGGCATCAAGCAAGGCATGATGCCCATGGAT
TATTCGCTCAGCCGCTTGGTGAAAGCAAACCAGATCACGCAGGCGGAAGCGATGTCGCACAGCATGGATAAGGAAACGCT
GCGTCGCTACCTGACCATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(51-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6U2Z4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.017

0.487
  pilT Legionella pneumophila strain Lp02

49.711

98.017

0.487
  pilT Legionella pneumophila strain ERS1305867

49.711

98.017

0.487
  pilT Neisseria meningitidis 8013

48.433

99.433

0.482
  pilT Neisseria gonorrhoeae MS11

48.148

99.433

0.479
  pilT Acinetobacter baumannii D1279779

48.547

97.45

0.473
  pilT Acinetobacter baumannii strain A118

48.547

97.45

0.473
  pilT Acinetobacter nosocomialis M2

48.547

97.45

0.473
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.405

95.184

0.47
  pilT Vibrio cholerae strain A1552

49.405

95.184

0.47
  pilT Acinetobacter baylyi ADP1

46.839

98.584

0.462
  pilT Pseudomonas stutzeri DSM 10701

46.552

98.584

0.459
  pilT Pseudomonas aeruginosa PAK

47.478

95.467

0.453
  pilU Pseudomonas stutzeri DSM 10701

41.279

97.45

0.402
  pilU Vibrio cholerae strain A1552

41.945

93.201

0.391
  pilU Acinetobacter baylyi ADP1

39.763

95.467

0.38

Multiple sequence alignment