Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBA65_RS11240 Genome accession   NZ_CP045121
Coordinates   2186562..2187704 (-) Length   380 a.a.
NCBI ID   WP_166396632.1    Uniprot ID   A0A6G8PXM4
Organism   Rubrobacter marinus strain SCSIO 52915     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2181562..2192704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBA65_RS11220 (GBA65_11025) - 2182054..2182539 (+) 486 WP_166396629.1 GNAT family N-acetyltransferase -
  GBA65_RS11225 (GBA65_11030) - 2182669..2183421 (-) 753 WP_166396630.1 sigma-70 family RNA polymerase sigma factor -
  GBA65_RS11230 (GBA65_11035) - 2184184..2184384 (-) 201 WP_166396631.1 hypothetical protein -
  GBA65_RS11235 (GBA65_11040) - 2185317..2186528 (-) 1212 WP_207955704.1 type II secretion system F family protein -
  GBA65_RS11240 (GBA65_11045) pilT 2186562..2187704 (-) 1143 WP_166396632.1 type IV pilus twitching motility protein PilT Machinery gene
  GBA65_RS11245 (GBA65_11050) - 2187795..2190851 (-) 3057 WP_166396633.1 ATPase, T2SS/T4P/T4SS family -
  GBA65_RS26430 - 2190915..2191421 (-) 507 WP_407691012.1 endolytic transglycosylase MltG -
  GBA65_RS25220 (GBA65_11060) mltG 2191480..2192220 (-) 741 WP_166396635.1 endolytic transglycosylase MltG -
  GBA65_RS26435 (GBA65_11070) ruvX 2192260..2192703 (-) 444 WP_166398470.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 41952.09 Da        Isoelectric Point: 7.6012

>NTDB_id=392072 GBA65_RS11240 WP_166396632.1 2186562..2187704(-) (pilT) [Rubrobacter marinus strain SCSIO 52915]
MMLESGLNDYLFDAIKAGASDLHITVGLPPMIRVHGKVQPLDYPPLTGQMTREMVYDILSNDQRQRLESEWELDFAYTLP
RTARFRVNVYFQRGSMGAAFRTIPHEIKDFSELGLPHAIEQMAEKPRGLVLVTGPTGSGKSTTLASMIDKINRERAEHIM
SVEDPIEFLHQHKKCIVNQREVNQDTKSFAQALKHVLRQDPDVILVGEMRDLETIGLAISAAETGHLVYGTLHTQDAPQT
VDRIIDVFPPSQQGQVRAQLAIALQGIVTQTLIPKRDGKGRVVACEILVPTPGVRNLIREGKNHQIYSAMQTGGKFGMQT
MDAALVELVRKGEISREEAEKRSSNPDELKRLMGGSGATPQAPVAAAPAKSGYAAFGSRR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=392072 GBA65_RS11240 WP_166396632.1 2186562..2187704(-) (pilT) [Rubrobacter marinus strain SCSIO 52915]
ATGATGCTAGAGAGCGGGCTCAACGACTACCTGTTCGACGCCATAAAAGCGGGGGCCAGCGACCTGCACATCACCGTGGG
CCTGCCCCCGATGATCCGGGTACACGGCAAGGTCCAGCCCCTGGACTACCCGCCGCTGACGGGCCAGATGACGCGCGAGA
TGGTCTACGACATCCTCTCCAACGACCAGCGCCAGCGGCTTGAGAGCGAGTGGGAGCTCGACTTCGCGTACACGCTGCCG
CGGACGGCCCGCTTCCGTGTCAACGTGTACTTTCAGCGCGGCTCGATGGGGGCGGCGTTCCGGACGATCCCGCACGAGAT
CAAGGACTTCAGCGAGCTCGGGCTCCCGCACGCGATCGAGCAGATGGCCGAGAAGCCGCGCGGGCTCGTCCTCGTCACGG
GGCCGACCGGCTCGGGCAAGTCGACCACCCTCGCCTCGATGATCGACAAGATCAACCGCGAGCGCGCCGAGCACATCATG
AGCGTGGAGGACCCCATAGAGTTCCTCCACCAGCACAAGAAGTGCATCGTGAACCAGCGCGAGGTCAACCAGGACACCAA
GTCCTTCGCCCAGGCCCTCAAGCACGTGCTCAGGCAGGACCCGGACGTCATCCTCGTCGGCGAGATGCGCGACCTCGAGA
CGATCGGGCTCGCCATCAGCGCGGCCGAGACCGGCCACCTCGTCTACGGCACCCTCCACACCCAGGACGCCCCGCAGACC
GTCGACCGGATCATCGACGTCTTCCCGCCGAGCCAGCAGGGCCAGGTCAGGGCGCAGCTCGCCATCGCGCTCCAGGGCAT
CGTGACGCAGACCCTGATCCCGAAGCGCGACGGCAAGGGGCGGGTCGTGGCCTGCGAGATCCTCGTGCCGACGCCGGGCG
TCAGGAACCTCATCCGCGAGGGGAAGAACCACCAGATCTACTCCGCCATGCAGACGGGCGGGAAGTTCGGGATGCAGACG
ATGGACGCCGCCCTCGTGGAGCTCGTCCGTAAGGGCGAGATCTCGCGCGAGGAGGCCGAGAAGCGCTCCAGCAACCCCGA
CGAGTTGAAGCGGCTGATGGGCGGCTCCGGCGCGACGCCGCAGGCGCCCGTAGCGGCGGCGCCTGCGAAATCCGGTTACG
CGGCCTTCGGAAGTCGGCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-251)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8PXM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.648

95.789

0.495
  pilT Pseudomonas aeruginosa PAK

53.644

90.263

0.484
  pilT Legionella pneumophila strain Lp02

54.819

87.368

0.479
  pilT Legionella pneumophila strain ERS1305867

54.819

87.368

0.479
  pilT Acinetobacter baumannii D1279779

52.478

90.263

0.474
  pilT Acinetobacter nosocomialis M2

52.478

90.263

0.474
  pilT Acinetobacter baumannii strain A118

52.478

90.263

0.474
  pilT Acinetobacter baylyi ADP1

51.603

90.263

0.466
  pilT Pseudomonas stutzeri DSM 10701

51.603

90.263

0.466
  pilT Neisseria gonorrhoeae MS11

51.603

90.263

0.466
  pilT Neisseria meningitidis 8013

51.603

90.263

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

86.579

0.426
  pilT Vibrio cholerae strain A1552

49.24

86.579

0.426
  pilU Pseudomonas stutzeri DSM 10701

45.428

89.211

0.405
  pilU Vibrio cholerae strain A1552

40.922

91.316

0.374
  pilU Acinetobacter baylyi ADP1

40.87

90.789

0.371

Multiple sequence alignment