Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBA63_RS10325 Genome accession   NZ_CP045119
Coordinates   2078791..2079912 (+) Length   373 a.a.
NCBI ID   WP_166175812.1    Uniprot ID   A0A6G8Q961
Organism   Rubrobacter tropicus strain SCSIO 52909     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2073791..2084912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBA63_RS24335 - 2074204..2074891 (+) 688 Protein_2058 sensor histidine kinase -
  GBA63_RS10315 (GBA63_10160) - 2075055..2076134 (-) 1080 WP_166175808.1 type IV pilus twitching motility protein PilT -
  GBA63_RS10320 (GBA63_10165) - 2076320..2078758 (+) 2439 WP_166175810.1 type II/IV secretion system protein -
  GBA63_RS10325 (GBA63_10170) pilT 2078791..2079912 (+) 1122 WP_166175812.1 type IV pilus twitching motility protein PilT Machinery gene
  GBA63_RS10330 (GBA63_10175) - 2079919..2081130 (+) 1212 WP_166175814.1 type II secretion system F family protein -
  GBA63_RS10335 (GBA63_10180) - 2081307..2081654 (+) 348 WP_166175816.1 DUF1778 domain-containing protein -
  GBA63_RS10340 (GBA63_10185) - 2081679..2082269 (+) 591 WP_207957190.1 GNAT family N-acetyltransferase -
  GBA63_RS10345 (GBA63_10190) rpsB 2082788..2083861 (+) 1074 WP_166175818.1 30S ribosomal protein S2 -
  GBA63_RS10350 (GBA63_10195) tsf 2083867..2084454 (+) 588 WP_166175820.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41227.95 Da        Isoelectric Point: 7.5763

>NTDB_id=392054 GBA63_RS10325 WP_166175812.1 2078791..2079912(+) (pilT) [Rubrobacter tropicus strain SCSIO 52909]
MIQTGLNDYLFDAIRMGASDLHITAGLPPMVRLSGKVQALDYEPFTPNATREMVYDILSSDQRQRLENEWELDFSYTLPR
TARFRVNVYFQKGAIGAAFRTIPHEIKTLGELGLPKAVEDMTERPRGLVLVTGPTGSGKSTTLASMIDRINETRSEHIMS
VEDPIEFLHAHKKCIVNQREVNQDTKSFAQALKHVLRQDPDVILVGEMRDLETISLAVTAAETGHLVFGTLHTQDAPQTV
DRIIDVFPSHQQSQIRTQLANALQGVITQTLVPRRGGEGRVAACEVLVPTPGVRNLIREGKNHQIYSAMQTGGKFGMQTM
DAALVDLLRRNLISREEAEKRASNPEELRRLAGGSSLGANSKSSAGGYAAFRK

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=392054 GBA63_RS10325 WP_166175812.1 2078791..2079912(+) (pilT) [Rubrobacter tropicus strain SCSIO 52909]
ATGATACAGACGGGGCTCAACGACTACCTTTTCGACGCGATCCGGATGGGGGCGAGCGACCTCCATATAACTGCGGGTCT
GCCCCCGATGGTGCGCCTGAGCGGCAAGGTGCAGGCGCTCGATTATGAGCCCTTCACCCCGAACGCGACCCGGGAGATGG
TCTACGACATCCTCTCAAGCGACCAGCGCCAGCGCCTGGAGAACGAGTGGGAGCTGGACTTCTCCTACACGCTGCCGCGC
ACGGCCCGCTTCAGGGTGAACGTCTACTTCCAGAAGGGCGCCATCGGGGCCGCCTTCAGGACCATCCCGCACGAGATAAA
GACCCTGGGAGAGCTCGGGCTGCCGAAGGCCGTCGAGGACATGACGGAGAGGCCGCGGGGGCTCGTCCTTGTAACGGGGC
CGACGGGGAGCGGCAAATCGACGACGCTCGCGTCCATGATCGACAGGATCAACGAGACCAGAAGCGAGCACATAATGAGC
GTGGAGGACCCGATAGAGTTCCTCCACGCCCACAAGAAGTGCATAGTCAACCAGCGCGAGGTAAACCAGGACACCAAGAG
CTTCGCCCAGGCGCTCAAGCACGTCCTGCGCCAGGACCCCGACGTGATCCTCGTCGGCGAGATGCGCGACCTGGAGACGA
TCTCGCTCGCCGTAACCGCCGCCGAGACCGGCCACCTCGTCTTCGGTACTCTCCACACCCAGGACGCACCCCAGACGGTC
GACCGCATCATAGACGTTTTCCCGTCCCACCAGCAGTCCCAGATCAGAACCCAGCTCGCAAACGCCCTGCAGGGCGTCAT
CACCCAGACGCTCGTGCCCCGGCGCGGGGGCGAGGGGAGGGTCGCCGCCTGCGAGGTGCTCGTCCCGACGCCGGGCGTCA
GGAACCTCATAAGGGAGGGGAAGAACCACCAGATCTACTCGGCCATGCAGACCGGCGGCAAGTTCGGGATGCAGACGATG
GACGCCGCCCTCGTCGACCTGTTGCGCCGGAACCTGATCTCCCGCGAAGAGGCCGAGAAGCGGGCCTCGAACCCCGAGGA
GTTGCGCCGTCTGGCCGGCGGCAGCTCCCTGGGCGCGAACTCGAAATCGAGCGCCGGCGGCTACGCGGCGTTCAGGAAGT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8Q961

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.866

95.979

0.536
  pilT Pseudomonas aeruginosa PAK

53.936

91.957

0.496
  pilT Acinetobacter baylyi ADP1

55.589

88.74

0.493
  pilT Pseudomonas stutzeri DSM 10701

53.353

91.957

0.491
  pilT Acinetobacter nosocomialis M2

53.061

91.957

0.488
  pilT Acinetobacter baumannii D1279779

53.061

91.957

0.488
  pilT Acinetobacter baumannii strain A118

53.061

91.957

0.488
  pilT Legionella pneumophila strain ERS1305867

53.313

89.008

0.475
  pilT Legionella pneumophila strain Lp02

53.313

89.008

0.475
  pilT Neisseria meningitidis 8013

51.312

91.957

0.472
  pilT Neisseria gonorrhoeae MS11

51.312

91.957

0.472
  pilT Vibrio cholerae strain A1552

51.064

88.204

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.064

88.204

0.45
  pilU Pseudomonas stutzeri DSM 10701

43.75

90.08

0.394
  pilU Vibrio cholerae strain A1552

41.279

92.225

0.381
  pilU Acinetobacter baylyi ADP1

40.87

92.493

0.378

Multiple sequence alignment