Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GBN63_RS01785 Genome accession   NZ_CP045098
Coordinates   386734..387750 (-) Length   338 a.a.
NCBI ID   WP_239767463.1    Uniprot ID   A0AAI9L0Q8
Organism   Pectobacterium carotovorum strain ZM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 381734..392750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN63_RS01770 (GBN63_01770) nsrR 382108..382533 (-) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -
  GBN63_RS01775 (GBN63_01775) - 382828..384399 (+) 1572 WP_039530827.1 methyl-accepting chemotaxis protein -
  GBN63_RS01780 (GBN63_01780) - 384933..386765 (+) 1833 WP_010294991.1 sensor domain-containing diguanylate cyclase -
  GBN63_RS01785 (GBN63_01785) pilT 386734..387750 (-) 1017 WP_239767463.1 type IV pilus twitching motility protein PilT Machinery gene
  GBN63_RS01790 (GBN63_01790) - 387772..388485 (+) 714 WP_201878023.1 YggS family pyridoxal phosphate-dependent enzyme -
  GBN63_RS01795 (GBN63_01795) proC 388583..389404 (+) 822 WP_226294506.1 pyrroline-5-carboxylate reductase -
  GBN63_RS01800 (GBN63_01800) - 389454..390008 (+) 555 WP_010295003.1 YggT family protein -
  GBN63_RS01805 (GBN63_01805) yggU 390005..390295 (+) 291 WP_010295005.1 DUF167 family protein YggU -
  GBN63_RS01810 (GBN63_01810) - 390327..390920 (+) 594 WP_039470191.1 XTP/dITP diphosphatase -
  GBN63_RS01815 (GBN63_01815) hemW 390913..392052 (+) 1140 WP_039470189.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36906.16 Da        Isoelectric Point: 6.5666

>NTDB_id=391827 GBN63_RS01785 WP_239767463.1 386734..387750(-) (pilT) [Pectobacterium carotovorum strain ZM1]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPPVFSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAEQGGRIAIYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=391827 GBN63_RS01785 WP_239767463.1 386734..387750(-) (pilT) [Pectobacterium carotovorum strain ZM1]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCATCCGCCGGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACACCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGTTGCGTGTTATCCCGTCCGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGATCGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATACCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTGC
ACAGGCACTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCAGTGGTGACGCA
AAAACTCTTGCCTGCGGAGCAAGGGGGGCGAATCGCGATTTATGAAGTGCTGACCGCCACCGCGGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTTGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.121

97.633

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

97.633

0.509
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Acinetobacter baylyi ADP1

49.547

97.929

0.485
  pilT Pseudomonas stutzeri DSM 10701

49.547

97.929

0.485
  pilT Pseudomonas aeruginosa PAK

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.943

97.929

0.479
  pilT Acinetobacter baumannii D1279779

48.943

97.929

0.479
  pilT Acinetobacter baumannii strain A118

48.943

97.929

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.84

99.704

0.417
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367

Multiple sequence alignment