Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALNI_RS08315 Genome accession   NC_014758
Coordinates   1706432..1707544 (-) Length   370 a.a.
NCBI ID   WP_013451765.1    Uniprot ID   -
Organism   Calditerrivibrio nitroreducens DSM 19672     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1701432..1712544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALNI_RS08300 (Calni_1644) - 1701678..1703696 (-) 2019 WP_013451762.1 alpha-amylase/4-alpha-glucanotransferase domain-containing protein -
  CALNI_RS08305 (Calni_1645) - 1703711..1705198 (-) 1488 WP_013451763.1 histidine kinase dimerization/phospho-acceptor domain-containing protein -
  CALNI_RS08310 (Calni_1646) - 1705195..1706460 (-) 1266 WP_245529717.1 type II secretion system F family protein -
  CALNI_RS08315 (Calni_1647) pilT 1706432..1707544 (-) 1113 WP_013451765.1 type IV pilus twitching motility protein PilT Machinery gene
  CALNI_RS08320 (Calni_1648) pilB 1707562..1709262 (-) 1701 WP_013451766.1 type IV-A pilus assembly ATPase PilB -
  CALNI_RS08325 (Calni_1649) aroE 1709272..1710063 (-) 792 WP_013451767.1 shikimate dehydrogenase -
  CALNI_RS08330 (Calni_1650) - 1710056..1710736 (-) 681 WP_049770130.1 DUF502 domain-containing protein -
  CALNI_RS08335 (Calni_1651) upp 1710711..1711346 (-) 636 WP_013451769.1 uracil phosphoribosyltransferase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41789.26 Da        Isoelectric Point: 7.0094

>NTDB_id=39096 CALNI_RS08315 WP_013451765.1 1706432..1707544(-) (pilT) [Calditerrivibrio nitroreducens DSM 19672]
MSEMTENQETSKYSLQELLQKMIELDATDLHLTVGTPPVYRIHGDLVRFGTEILLPNDTKKICYGIMTEAQKKRFEEDQE
LDCSFGIKGVSRFRANFFMQRGAVAAAIRRIPYRVMTFEELGLPPVLKTLCEKPRGLVLVTGPTGSGKSTTLAAMIDKIN
SEQKVHIITIEDPIEYLHQHKEAMVNQREVNADTKSFAKALKYILRQDPDVVLIGEMRDLETIEAALTVAETGHLTFGTL
HTNSAIQTINRIIDVFPPHQQSQVRAQLSFVLEGVVCQQLLQRADGKGRVMVCEVLIPNPGIRNLIREDKLHQIYSMMQS
GQAEHGMITMSQSLFNAYAKGLITYDEAVNRAVYPEEVKQMIDRSGIRRR

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=39096 CALNI_RS08315 WP_013451765.1 1706432..1707544(-) (pilT) [Calditerrivibrio nitroreducens DSM 19672]
ATGTCTGAGATGACTGAAAATCAAGAAACTAGTAAGTACTCCCTGCAGGAGTTATTGCAGAAGATGATAGAGCTTGATGC
CACAGACCTACATCTTACGGTGGGCACTCCACCAGTTTATAGGATACACGGAGACCTGGTAAGATTTGGTACAGAGATTC
TTCTGCCAAATGATACCAAAAAGATATGTTATGGTATTATGACTGAGGCACAGAAGAAGAGGTTTGAGGAGGATCAGGAG
TTGGATTGCTCCTTTGGTATAAAAGGGGTTAGTAGATTTAGAGCAAACTTTTTTATGCAAAGGGGTGCTGTGGCTGCTGC
AATAAGAAGAATCCCATATCGTGTAATGACTTTTGAAGAGCTTGGGTTGCCACCTGTTCTCAAGACCCTCTGTGAAAAAC
CAAGAGGTTTGGTTCTTGTAACTGGTCCCACCGGAAGTGGTAAGTCTACTACACTTGCAGCTATGATAGACAAAATAAAT
TCTGAACAAAAGGTACACATAATCACAATAGAAGACCCTATAGAGTATTTACATCAGCATAAAGAGGCAATGGTAAATCA
GAGGGAGGTAAATGCTGATACTAAGTCGTTTGCCAAAGCGTTGAAATATATATTAAGGCAGGATCCTGATGTAGTGTTGA
TAGGTGAGATGAGGGATCTTGAAACTATAGAAGCTGCTCTTACAGTTGCAGAAACCGGACACCTTACTTTTGGAACCCTT
CATACAAACAGTGCTATCCAGACTATAAATAGAATTATAGATGTTTTCCCGCCACACCAACAGTCACAGGTTAGAGCACA
ACTTTCTTTTGTTTTAGAGGGGGTTGTATGTCAACAGCTACTTCAAAGAGCTGATGGTAAAGGTAGGGTTATGGTATGCG
AGGTTTTAATTCCTAATCCAGGTATTAGAAACCTTATAAGAGAAGATAAACTACATCAGATCTACTCTATGATGCAATCC
GGTCAGGCGGAACATGGGATGATTACCATGAGTCAATCACTCTTTAACGCTTATGCTAAAGGGTTAATTACTTACGATGA
AGCTGTAAATAGAGCAGTATATCCTGAAGAGGTTAAGCAGATGATTGATAGGTCTGGTATCAGAAGGAGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(46-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.26

95.676

0.5
  pilT Legionella pneumophila strain ERS1305867

51.453

92.973

0.478
  pilT Legionella pneumophila strain Lp02

51.453

92.973

0.478
  pilT Pseudomonas stutzeri DSM 10701

51.17

92.432

0.473
  pilT Pseudomonas aeruginosa PAK

51.17

92.432

0.473
  pilT Acinetobacter baumannii D1279779

51.17

92.432

0.473
  pilT Acinetobacter baumannii strain A118

51.17

92.432

0.473
  pilT Acinetobacter nosocomialis M2

50.877

92.432

0.47
  pilT Acinetobacter baylyi ADP1

50.585

92.432

0.468
  pilT Neisseria gonorrhoeae MS11

50.45

90

0.454
  pilT Neisseria meningitidis 8013

50.45

90

0.454
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.949

90

0.441
  pilT Vibrio cholerae strain A1552

48.949

90

0.441
  pilU Pseudomonas stutzeri DSM 10701

43.966

94.054

0.414
  pilU Vibrio cholerae strain A1552

43.966

94.054

0.414
  pilU Acinetobacter baylyi ADP1

40.395

95.676

0.386

Multiple sequence alignment