Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALNI_RS06045 Genome accession   NC_014758
Coordinates   1266212..1267276 (-) Length   354 a.a.
NCBI ID   WP_013451327.1    Uniprot ID   E4TIY3
Organism   Calditerrivibrio nitroreducens DSM 19672     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1261212..1272276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALNI_RS06020 (Calni_1202) - 1261474..1262109 (-) 636 WP_013451322.1 response regulator transcription factor -
  CALNI_RS06025 (Calni_1203) - 1262106..1262732 (-) 627 WP_013451323.1 MBL fold metallo-hydrolase -
  CALNI_RS06030 (Calni_1204) dtd 1262743..1263186 (-) 444 WP_013451324.1 D-aminoacyl-tRNA deacylase -
  CALNI_RS06035 (Calni_1205) murJ 1263183..1264667 (-) 1485 WP_013451325.1 murein biosynthesis integral membrane protein MurJ -
  CALNI_RS06040 (Calni_1206) - 1264664..1266211 (-) 1548 WP_013451326.1 NFACT RNA binding domain-containing protein -
  CALNI_RS06045 (Calni_1207) pilT 1266212..1267276 (-) 1065 WP_013451327.1 type IV pilus twitching motility protein PilT Machinery gene
  CALNI_RS06050 (Calni_1208) - 1267287..1268096 (-) 810 WP_013451328.1 hypothetical protein -
  CALNI_RS06055 (Calni_1209) - 1268089..1269081 (-) 993 WP_013451329.1 hypothetical protein -
  CALNI_RS06060 (Calni_1210) - 1269082..1269762 (-) 681 WP_013451330.1 3'-5' exonuclease -
  CALNI_RS06065 (Calni_1211) - 1269895..1270740 (-) 846 WP_013451331.1 DUF4384 domain-containing protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39843.32 Da        Isoelectric Point: 7.1095

>NTDB_id=39094 CALNI_RS06045 WP_013451327.1 1266212..1267276(-) (pilT) [Calditerrivibrio nitroreducens DSM 19672]
MAKIDAFFKYMMENNCSDLHLSSGCKPMVRKHGELEEIKYQELTNEILEKLLFEIITDEQRDFFLKKKDLDFAYEIPGMA
RFRANYFYQKRGIAAVFRIIPSKILTAEELGLPPQVLKFANLSRGLVLVTGPTGSGKSTTLAAIIDYINRNRKDHILTIE
DPVEFVHESKGCLVNHREVGSMTQSFSAALRAALREDPDVILVGEMRDLETIELAITAAETGHLVFGTLHTNSAPKTVDR
IIDAFPAGQQAQIRTMLSESLKGVISQQLLKRSDKPGRVAALEIMVVNSAIANLIREGKIFQIPSIMQTGKADGMQMMDQ
VILDFLMNKVISPEEAYLKANDKKMFEKFLQQKQ

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=39094 CALNI_RS06045 WP_013451327.1 1266212..1267276(-) (pilT) [Calditerrivibrio nitroreducens DSM 19672]
ATGGCTAAGATAGATGCTTTTTTCAAGTATATGATGGAAAATAATTGTAGTGATCTGCATCTGAGCAGCGGGTGTAAACC
TATGGTGAGGAAACATGGTGAATTGGAGGAGATAAAGTATCAGGAGCTGACCAATGAGATCCTCGAAAAGCTCCTTTTTG
AGATTATAACTGATGAACAAAGGGACTTTTTTCTGAAGAAAAAGGATCTGGATTTTGCCTATGAAATACCCGGGATGGCG
AGATTCAGGGCAAACTATTTTTATCAGAAAAGGGGGATCGCGGCTGTTTTCAGGATTATCCCATCTAAGATTCTAACAGC
GGAAGAATTGGGTTTACCTCCGCAGGTGCTAAAGTTTGCCAATCTTTCGAGGGGGCTTGTCCTGGTCACAGGTCCAACAG
GGAGTGGAAAATCCACCACACTTGCGGCAATTATAGATTATATAAACAGAAATAGGAAAGACCACATTCTTACGATTGAA
GATCCGGTAGAGTTTGTTCATGAAAGCAAAGGTTGTCTTGTAAACCACAGGGAGGTTGGGTCGATGACACAATCGTTTAG
CGCTGCGTTGAGGGCTGCCCTAAGAGAGGATCCGGATGTTATTCTTGTGGGTGAGATGAGGGATCTGGAGACGATAGAGC
TTGCCATCACAGCGGCGGAGACTGGTCATCTTGTTTTTGGAACTCTCCATACCAATTCTGCCCCCAAAACTGTTGACAGG
ATTATAGATGCTTTCCCCGCAGGACAACAGGCTCAAATTAGAACGATGCTTTCAGAGTCTTTAAAAGGTGTTATTTCCCA
GCAGCTTTTAAAAAGGTCTGATAAGCCTGGTAGGGTGGCTGCTCTTGAGATAATGGTGGTAAATAGTGCAATTGCAAATC
TAATAAGGGAAGGGAAGATATTTCAGATCCCATCCATAATGCAAACCGGTAAGGCTGATGGTATGCAGATGATGGATCAG
GTGATTCTGGACTTTTTAATGAATAAAGTCATTTCTCCCGAAGAGGCTTATCTCAAGGCGAATGATAAGAAGATGTTTGA
AAAGTTTCTACAGCAGAAGCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4TIY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

58.017

96.893

0.562
  pilT Legionella pneumophila strain ERS1305867

58.017

96.893

0.562
  pilT Acinetobacter baylyi ADP1

55.977

96.893

0.542
  pilT Acinetobacter baumannii D1279779

55.977

96.893

0.542
  pilT Acinetobacter baumannii strain A118

55.977

96.893

0.542
  pilT Acinetobacter nosocomialis M2

55.977

96.893

0.542
  pilT Neisseria meningitidis 8013

55.848

96.61

0.54
  pilT Pseudomonas stutzeri DSM 10701

55.685

96.893

0.54
  pilT Neisseria gonorrhoeae MS11

55.556

96.61

0.537
  pilT Pseudomonas aeruginosa PAK

56.38

95.198

0.537
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.06

94.915

0.523
  pilT Vibrio cholerae strain A1552

55.06

94.915

0.523
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

97.74

0.483
  pilU Vibrio cholerae strain A1552

39.205

99.435

0.39
  pilU Pseudomonas stutzeri DSM 10701

38.571

98.87

0.381
  pilU Acinetobacter baylyi ADP1

39.286

94.915

0.373

Multiple sequence alignment