Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FSC11_RS11285 Genome accession   NZ_CP044483
Coordinates   2358928..2359965 (+) Length   345 a.a.
NCBI ID   WP_004811122.1    Uniprot ID   A0A1P8PHZ9
Organism   Acinetobacter schindleri strain HZE30-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2316714..2358071 2358928..2359965 flank 857


Gene organization within MGE regions


Location: 2316714..2359965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC11_RS11120 (FSC11_11135) - 2316714..2317256 (-) 543 WP_163166907.1 DUF2799 domain-containing protein -
  FSC11_RS11125 (FSC11_11140) - 2317331..2317996 (-) 666 WP_227590982.1 hypothetical protein -
  FSC11_RS11130 (FSC11_11145) - 2318020..2319459 (-) 1440 WP_163166911.1 hypothetical protein -
  FSC11_RS11135 (FSC11_11150) - 2319538..2323260 (-) 3723 WP_163166913.1 tubulin-like doman-containing protein -
  FSC11_RS11140 (FSC11_11155) - 2323312..2323722 (-) 411 WP_163166915.1 hypothetical protein -
  FSC11_RS11145 (FSC11_11160) - 2323763..2325226 (-) 1464 WP_163166917.1 hypothetical protein -
  FSC11_RS11150 (FSC11_11165) - 2325223..2326701 (-) 1479 WP_227590983.1 hypothetical protein -
  FSC11_RS11155 (FSC11_11170) - 2327245..2330121 (-) 2877 WP_163166921.1 type I restriction endonuclease subunit R -
  FSC11_RS11160 (FSC11_11175) - 2330150..2331061 (-) 912 WP_163166923.1 DUF5655 domain-containing protein -
  FSC11_RS11165 (FSC11_11180) - 2331071..2332294 (-) 1224 WP_163166925.1 restriction endonuclease subunit S -
  FSC11_RS11170 (FSC11_11185) - 2332294..2333868 (-) 1575 WP_163166927.1 type I restriction-modification system subunit M -
  FSC11_RS11175 (FSC11_11190) - 2334072..2335133 (-) 1062 WP_163166930.1 HNH endonuclease -
  FSC11_RS11180 (FSC11_11195) - 2335136..2335723 (-) 588 WP_163166932.1 class I SAM-dependent methyltransferase -
  FSC11_RS11185 (FSC11_11200) - 2335796..2336026 (-) 231 WP_005096077.1 helix-turn-helix transcriptional regulator -
  FSC11_RS11190 (FSC11_11205) - 2336122..2337024 (-) 903 WP_163166934.1 hydrolase or metal-binding protein -
  FSC11_RS11195 (FSC11_11210) - 2337200..2338264 (-) 1065 WP_111281997.1 YqaJ viral recombinase family protein -
  FSC11_RS11200 (FSC11_11215) - 2338416..2339450 (-) 1035 WP_111281999.1 DUF932 domain-containing protein -
  FSC11_RS11205 (FSC11_11220) - 2339495..2339782 (-) 288 WP_032068291.1 hypothetical protein -
  FSC11_RS11210 (FSC11_11225) - 2339916..2340863 (-) 948 WP_075040455.1 YagK/YfjJ domain-containing protein -
  FSC11_RS11215 (FSC11_11230) - 2341150..2342769 (-) 1620 WP_163166936.1 hypothetical protein -
  FSC11_RS11220 (FSC11_11235) - 2342891..2343886 (+) 996 WP_163166938.1 hypothetical protein -
  FSC11_RS11225 (FSC11_11240) - 2343880..2345019 (-) 1140 WP_032068297.1 tyrosine-type recombinase/integrase -
  FSC11_RS11235 (FSC11_11250) glpK 2345694..2347178 (+) 1485 WP_163166940.1 glycerol kinase GlpK -
  FSC11_RS11240 (FSC11_11255) - 2347230..2347721 (-) 492 WP_004893342.1 thioesterase family protein -
  FSC11_RS11245 (FSC11_11260) hemB 2347829..2348842 (+) 1014 WP_163166942.1 porphobilinogen synthase -
  FSC11_RS11250 (FSC11_11265) thiO 2348926..2350047 (+) 1122 WP_163166944.1 glycine oxidase ThiO -
  FSC11_RS11255 (FSC11_11270) - 2350027..2350938 (-) 912 WP_163166946.1 TIGR01777 family oxidoreductase -
  FSC11_RS11260 (FSC11_11275) - 2351119..2351574 (-) 456 WP_004893338.1 ABZJ_00895 family protein -
  FSC11_RS11265 (FSC11_11280) - 2352094..2353344 (+) 1251 WP_163166948.1 exonuclease SbcCD subunit D -
  FSC11_RS11270 (FSC11_11285) - 2353354..2356959 (+) 3606 WP_163166950.1 AAA family ATPase -
  FSC11_RS11275 (FSC11_11290) - 2356965..2358071 (+) 1107 WP_163166952.1 acyltransferase family protein -
  FSC11_RS11280 (FSC11_11295) - 2358118..2358804 (-) 687 WP_163167788.1 YggS family pyridoxal phosphate-dependent enzyme -
  FSC11_RS11285 (FSC11_11300) pilT 2358928..2359965 (+) 1038 WP_004811122.1 type IV pilus twitching motility protein PilT Machinery gene

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38209.77 Da        Isoelectric Point: 6.5054

>NTDB_id=390259 FSC11_RS11285 WP_004811122.1 2358928..2359965(+) (pilT) [Acinetobacter schindleri strain HZE30-1]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICDYPRGLVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVVSQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=390259 FSC11_RS11285 WP_004811122.1 2358928..2359965(+) (pilT) [Acinetobacter schindleri strain HZE30-1]
ATGGACATTACAGAATTATTAGCATTTTCTGCTAAAAATGGCGCGTCAGATTTACACTTGTCTGCGGGGATGCCACCACT
GATTCGTGTTGATGGTGAAGTTCGTCGTATTAACCTGCCTGCCCTGGAACATAAAGAAGTTCACAAGCTGGTTTACGATA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAATCTAGAAACTGACTTTTCCTTTGAAGTTCCTGGCGTCGCCCGT
TTCCGTGTGAACGCATTTAACCAGAACCGCGGTGCCGGTGCCGTATTCCGTACCATTCCCTCTAAAGTTTTAACCATTGA
AGATCTGGGCATGGGGCAGATTTTTAAAGATATTTGTGACTATCCGCGTGGTCTGGTACTGGTCACAGGCCCAACCGGTT
CAGGTAAATCAACGACGCTGGCAGCGATGCTGGACTATATTAATGATAACCGTTATGACCATATTCTGACCGTCGAAGAT
CCAATTGAATTTGTACACCAATCCAAAAAATGTCTGATCAACCAGCGTGAAGTGCATCGTGACACGCATGGTTTCAATGA
AGCATTACGTTCTGCATTACGTGAAGACCCGGATATTATCCTGGTGGGTGAGATGCGTGACCTTGAAACCATCCGTCTGG
CACTCACTGCTGCTGAAACCGGTCACCTGGTGTTTGGCACCCTGCATACGACTTCTGCTGCTAAAACTATTGACCGTGTG
ATCGACGTATTCCCGGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCATTACAAGCGGTTGTCTCACAAAC
CCTGCTCAAGAAAAATGGTGGTGGTCGTGTCGCTGCACATGAAATCATGATCGGTATTCCGGCCATCCGTAACCTGATCC
GCGAAAACAAAGTTGCTCAAATGTACTCTGCCATTCAGACCGGTGCCAACTATGGTATGACTACGCTGGATCAGAGCCTG
AAAACACTGGTATCTAAAGGCGTGATCAGTCCACAAGTTGCGCGTACTGCTGCTAAACAGCCTGAATCTTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1P8PHZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.652

100

0.957
  pilT Acinetobacter baumannii D1279779

95.362

100

0.954
  pilT Acinetobacter baumannii strain A118

95.362

100

0.954
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.692

95.942

0.4
  pilU Acinetobacter baylyi ADP1

38.15

100

0.383

Multiple sequence alignment