Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FSC12_RS03905 Genome accession   NZ_CP044474
Coordinates   830474..831595 (+) Length   373 a.a.
NCBI ID   WP_163168564.1    Uniprot ID   -
Organism   Acinetobacter schindleri strain HZE33-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825474..836595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FSC12_RS03890 (FSC12_03910) - 827443..828549 (+) 1107 WP_163168562.1 acyltransferase -
  FSC12_RS03895 (FSC12_03915) - 828596..829282 (-) 687 WP_163169742.1 YggS family pyridoxal phosphate-dependent enzyme -
  FSC12_RS03900 (FSC12_03920) pilT 829406..830443 (+) 1038 WP_163168563.1 type IV pilus twitching motility protein PilT Machinery gene
  FSC12_RS03905 (FSC12_03925) pilU 830474..831595 (+) 1122 WP_163168564.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FSC12_RS03910 (FSC12_03930) fur 831670..832107 (-) 438 WP_004811125.1 ferric iron uptake transcriptional regulator -
  FSC12_RS03915 (FSC12_03935) - 832220..832618 (+) 399 WP_004811126.1 outer membrane protein assembly factor BamE -
  FSC12_RS03920 (FSC12_03940) - 832728..833054 (-) 327 WP_163168565.1 RnfH family protein -
  FSC12_RS03925 (FSC12_03945) - 833054..834133 (-) 1080 WP_163168566.1 hypothetical protein -
  FSC12_RS03930 (FSC12_03950) - 834273..834728 (-) 456 WP_004893331.1 bacteriohemerythrin -
  FSC12_RS03935 (FSC12_03955) - 835005..835853 (-) 849 WP_008305539.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41424.63 Da        Isoelectric Point: 6.8885

>NTDB_id=390193 FSC12_RS03905 WP_163168564.1 830474..831595(+) (pilU) [Acinetobacter schindleri strain HZE33-1]
MDFNGLLDYMVAQKASDLFITADVEPSIKVNGSIVPIGSVKLPGPAVGQILHSIMTEKQRKEFADTRECNFAITNPSKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDDLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASLIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTIDGTGRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGAHLMNAGSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=390193 FSC12_RS03905 WP_163168564.1 830474..831595(+) (pilU) [Acinetobacter schindleri strain HZE33-1]
ATGGATTTTAACGGCTTACTCGACTATATGGTGGCGCAAAAGGCATCGGATCTGTTCATTACTGCAGATGTCGAACCTTC
AATTAAAGTGAATGGCTCAATAGTGCCAATTGGATCAGTCAAATTACCTGGACCAGCAGTAGGCCAGATTCTGCATTCAA
TTATGACGGAAAAACAGCGCAAGGAATTTGCCGATACACGTGAGTGTAACTTTGCGATTACCAATCCCAGCAAATCAGCA
CGTTTCCGTGTCAGTGCATTTCAGCAGCGTGATCAGCCGGGTATGGTTCTGCGTCGTATTGAAACTGTCATTCCCACTAT
GGATGATTTAAAGTTACCACCAATTCTGAAAGAACTGGCGATGACCAAGCGCGGTATTATCATCTTCGTAGGCGCAACAG
GTACCGGTAAATCGACTTCCCTTGCCTCTTTGATTGGTTATCGTAATGAAAATTCCAAAGGTCATATCATTACCATCGAA
GACCCGATCGAATTTGTGCACCAGCATAAGGGGTGTATCATCACCCAGCGTGAAGTCGGGATCGACACAGATTCATTTGA
AATCGCCCTGAAAAATACCCTGCGTCAGGCACCAGACGTGATTCTGATCGGTGAGATCCGTTCCCGTGAAACCATGGACT
ATGCTATTGCCTTTGCGGAAACCGGTCACCTGGTATTTGCGACCCTACACGCCAATAACGCCAACCAGGCCATTGACCGG
ATTATTCACTTCTTTGAAGCGGACCGTCATAGCCAGCTGTTTATGGACTTGTCTTTAAACCTGAAAGCGATGGTGGCACA
GCAGCTGATCCCAACGATTGATGGTACAGGCCGGCGTGCCGCAATTGAGATCCTGATCAACTCACCTTTAGTTTCAGATC
TGATCCGTAAAGGTGATGTGCATGAAATTAAAGACTTGATGAAGCGTTCACGTGAACTTGGAATGCAGACTTTTGACCAG
GCACTGTATGACCTGTACAAGGCCAAACAGATCAGCTATAAGGATGCGCTGAAACATGCTGACTCTCCAAACGATCTGCG
TCTGCAAATCAAGTTATCCGAAGAAGGTGGTGCACATCTGATGAATGCCGGCAGCAATATTACCTTTGATGGACAATCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.545

100

0.745
  pilU Pseudomonas stutzeri DSM 10701

67.925

99.464

0.676
  pilU Vibrio cholerae strain A1552

57.265

94.102

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.322

97.319

0.402
  pilT Legionella pneumophila strain Lp02

44.048

90.08

0.397
  pilT Legionella pneumophila strain ERS1305867

44.048

90.08

0.397
  pilT Acinetobacter baumannii D1279779

42.274

91.957

0.389
  pilT Acinetobacter baumannii strain A118

42.274

91.957

0.389
  pilT Acinetobacter nosocomialis M2

42.274

91.957

0.389
  pilT Pseudomonas aeruginosa PAK

40.751

92.761

0.378
  pilT Neisseria meningitidis 8013

40.462

92.761

0.375
  pilT Neisseria gonorrhoeae MS11

40.462

92.761

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.173

92.761

0.373
  pilT Acinetobacter baylyi ADP1

40.233

91.957

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.031

94.102

0.367
  pilT Vibrio cholerae strain A1552

39.031

94.102

0.367

Multiple sequence alignment