Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALKR_RS04070 Genome accession   NC_014721
Coordinates   881203..882264 (+) Length   353 a.a.
NCBI ID   WP_013432158.1    Uniprot ID   E4S4A0
Organism   Caldicellulosiruptor acetigenus I77R1B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 876203..887264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALKR_RS04060 (Calkr_0812) - 876352..879387 (+) 3036 WP_013432156.1 response regulator -
  CALKR_RS04065 (Calkr_0813) - 879453..881168 (+) 1716 WP_013432157.1 GspE/PulE family protein -
  CALKR_RS04070 (Calkr_0814) pilT 881203..882264 (+) 1062 WP_013432158.1 type IV pilus twitching motility protein PilT Machinery gene
  CALKR_RS04075 (Calkr_0815) - 882335..883546 (+) 1212 WP_013432159.1 type II secretion system F family protein -
  CALKR_RS04080 (Calkr_0816) - 883569..883991 (+) 423 WP_013432160.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALKR_RS04085 (Calkr_0817) - 884026..884460 (+) 435 WP_013432161.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALKR_RS04090 (Calkr_0818) - 884530..885315 (+) 786 WP_013432162.1 A24 family peptidase -
  CALKR_RS04095 (Calkr_0819) - 885321..885626 (+) 306 WP_013432163.1 late competence development ComFB family protein -
  CALKR_RS04100 (Calkr_0820) - 885607..886152 (+) 546 WP_013432164.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALKR_RS04105 (Calkr_0821) - 886149..886706 (+) 558 WP_013432165.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39497.70 Da        Isoelectric Point: 6.7096

>NTDB_id=38951 CALKR_RS04070 WP_013432158.1 881203..882264(+) (pilT) [Caldicellulosiruptor acetigenus I77R1B]
MEPSINAILTDAFLKEASDIHITPGVPPVFRIHGRLVKADESILTPEMVEEFVREIVSEEQWKVLNEKGEVDFSYSIKGV
SRFRVNVYKQRGTFSIAFRIIPVNIPAFETLGLPPVLKEFTKLNKGLVLVTGPTGSGKSTTLASLIDIINTERDLHIITL
EDPIEYLHKHKKSIVNQREIGADTQSFANALRAALREDPDVILVGEMRDLETIAIALTAAETGHVVFSTLHTVGAAKTID
RIIDVFPPYQQLQIRVQLSTVLQGVVSQQLLTRRDGKGRVVATEVMIVNAAIRNLIREGKTFQIQSVIQTHQKTGMITME
QSLLDLYRRGLITREDVFNYATDIEFMQKLLSA

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=38951 CALKR_RS04070 WP_013432158.1 881203..882264(+) (pilT) [Caldicellulosiruptor acetigenus I77R1B]
ATGGAACCAAGTATAAATGCAATTCTGACAGATGCTTTTTTGAAGGAAGCATCGGACATACATATCACACCAGGTGTTCC
ACCTGTGTTTCGTATTCATGGGAGGCTTGTTAAAGCAGATGAATCGATACTAACTCCCGAGATGGTTGAAGAATTTGTCA
GAGAAATTGTTTCGGAAGAACAGTGGAAAGTATTAAATGAAAAAGGGGAAGTTGACTTTTCATATAGTATAAAGGGTGTC
AGTCGTTTTAGGGTTAATGTTTATAAACAGAGAGGCACCTTTTCAATAGCATTTAGAATCATCCCTGTTAACATACCAGC
TTTTGAAACGTTGGGCTTGCCTCCTGTCTTAAAAGAATTTACCAAGCTAAACAAAGGACTTGTATTGGTAACCGGTCCTA
CTGGCTCAGGAAAATCTACAACTCTTGCTTCGCTGATTGACATTATTAATACAGAACGGGACTTGCATATCATAACTTTG
GAAGATCCGATTGAATACTTACACAAGCACAAAAAAAGCATTGTTAATCAGAGGGAAATTGGGGCAGACACCCAAAGTTT
TGCCAATGCTTTGAGAGCAGCATTGAGAGAAGATCCAGATGTTATTCTTGTGGGCGAGATGAGAGATTTAGAAACAATTG
CTATTGCACTTACTGCGGCAGAGACAGGTCACGTGGTCTTTTCTACACTTCACACAGTTGGTGCTGCAAAAACAATAGAT
AGAATCATAGACGTTTTTCCACCCTATCAGCAGCTTCAGATTCGAGTTCAGCTTTCTACAGTACTGCAGGGGGTTGTATC
TCAACAGCTTCTAACCAGAAGGGATGGAAAAGGAAGAGTAGTTGCAACCGAAGTAATGATTGTAAATGCAGCTATAAGAA
ACCTTATAAGAGAAGGCAAAACATTCCAAATTCAATCTGTGATTCAGACACATCAAAAAACAGGCATGATTACGATGGAG
CAGTCACTTTTAGATCTTTACAGAAGAGGATTAATTACCAGAGAAGACGTTTTTAATTATGCTACTGACATTGAGTTTAT
GCAGAAGCTATTGAGTGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4S4A0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.734

98.017

0.507
  pilT Neisseria meningitidis 8013

51.497

94.618

0.487
  pilT Neisseria gonorrhoeae MS11

50.751

94.334

0.479
  pilT Legionella pneumophila strain Lp02

52.978

90.368

0.479
  pilT Legionella pneumophila strain ERS1305867

52.978

90.368

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.336

90.935

0.476
  pilT Vibrio cholerae strain A1552

52.336

90.935

0.476
  pilT Acinetobacter baylyi ADP1

50.76

93.201

0.473
  pilT Acinetobacter baumannii D1279779

52.366

89.802

0.47
  pilT Acinetobacter nosocomialis M2

52.366

89.802

0.47
  pilT Acinetobacter baumannii strain A118

52.366

89.802

0.47
  pilT Pseudomonas aeruginosa PAK

51.09

90.935

0.465
  pilT Pseudomonas stutzeri DSM 10701

51.097

90.368

0.462
  pilU Pseudomonas stutzeri DSM 10701

42.229

96.601

0.408
  pilU Vibrio cholerae strain A1552

38.218

98.584

0.377
  pilU Acinetobacter baylyi ADP1

39.755

92.635

0.368

Multiple sequence alignment