Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALOW_RS08065 Genome accession   NC_014657
Coordinates   1672790..1673845 (-) Length   351 a.a.
NCBI ID   WP_013412487.1    Uniprot ID   E4Q3R8
Organism   Caldicellulosiruptor owensensis OL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667790..1678845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALOW_RS08030 (Calow_1595) - 1668415..1668915 (-) 501 WP_013412480.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALOW_RS12255 (Calow_1596) - 1668912..1669517 (-) 606 WP_013412481.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALOW_RS08040 (Calow_1597) - 1669545..1669814 (-) 270 WP_013412482.1 late competence development ComFB family protein -
  CALOW_RS08045 (Calow_1598) - 1669847..1670605 (-) 759 WP_013412483.1 A24 family peptidase -
  CALOW_RS08050 (Calow_1599) - 1670641..1671099 (-) 459 WP_013412484.1 type II secretion system protein -
  CALOW_RS08055 (Calow_1600) - 1671122..1671544 (-) 423 WP_013412485.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CALOW_RS08060 (Calow_1601) - 1671560..1672771 (-) 1212 WP_013412486.1 type II secretion system F family protein -
  CALOW_RS08065 (Calow_1602) pilT 1672790..1673845 (-) 1056 WP_013412487.1 type IV pilus twitching motility protein PilT Machinery gene
  CALOW_RS08070 (Calow_1603) - 1673911..1675608 (-) 1698 WP_013412488.1 GspE/PulE family protein -
  CALOW_RS11650 (Calow_1604) - 1675659..1678730 (-) 3072 WP_013412489.1 response regulator -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39347.47 Da        Isoelectric Point: 9.0521

>NTDB_id=38902 CALOW_RS08065 WP_013412487.1 1672790..1673845(-) (pilT) [Caldicellulosiruptor owensensis OL]
MDINEILKEAFLKGASDIHITAGSPPIMRVHGVLTRMSDLSLTPEITERFVKEITNEYQYKRLQEAGEVDFSYSIRGLSR
FRVNAYKQRGSVAIAFRVISQDIPKFETLGLPPVLKDFTKLNKGLVLVTGPTGSGKSTTLASLIDIINTERNLHIITLED
PIEYLHRHKKSIVNQREVGNDTQSFATALRAALREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTVGAAKTIDRI
IDVFPPYQQQQIRVQLSTVLQAVVSQQLLTRRDGRGRVAAVEIMIANSAIRNLIREAKTYQIQSVIQTNTKMGMITMEQS
LVDLYKRGLITREDAIMYASDQELINKLLIF

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=38902 CALOW_RS08065 WP_013412487.1 1672790..1673845(-) (pilT) [Caldicellulosiruptor owensensis OL]
ATGGATATCAACGAGATTTTGAAAGAGGCATTTTTGAAAGGCGCATCTGATATACATATCACAGCAGGTTCGCCCCCTAT
CATGAGGGTGCACGGTGTTCTTACGCGCATGAGCGATTTGAGCCTCACACCAGAGATAACTGAAAGGTTTGTAAAAGAGA
TTACAAACGAATACCAGTACAAACGTTTGCAAGAAGCTGGTGAGGTTGACTTTTCTTACAGCATAAGAGGTTTGAGCAGG
TTTAGGGTAAATGCATATAAACAGCGTGGTTCTGTTGCTATTGCATTCAGAGTCATATCTCAGGATATACCAAAGTTTGA
GACGTTGGGGCTTCCTCCAGTTTTGAAGGATTTTACAAAGCTCAACAAAGGGCTTGTGCTTGTTACTGGTCCCACAGGTT
CAGGAAAATCGACCACATTAGCATCGCTTATAGATATAATAAACACTGAGAGAAACTTACATATTATTACATTGGAAGAT
CCTATTGAGTATCTTCATAGGCACAAAAAGAGTATTGTTAATCAAAGGGAAGTAGGGAACGACACGCAGAGTTTTGCAAC
GGCGCTGAGGGCAGCTTTGCGTGAGGACCCTGATGTAATTCTGGTTGGCGAGATGAGAGACTTAGAGACAATTTCCATAG
CACTTACCGCTGCAGAGACCGGGCACTTGGTTTTTTCAACACTTCACACTGTTGGTGCAGCAAAGACCATTGACAGAATC
ATTGACGTTTTCCCACCCTATCAACAACAGCAAATACGCGTTCAGCTTTCAACAGTTCTTCAAGCAGTTGTATCGCAACA
GCTTTTGACAAGGAGAGACGGGAGAGGCAGAGTTGCTGCTGTTGAAATTATGATAGCAAATTCAGCTATAAGAAATCTTA
TAAGAGAGGCTAAGACATATCAGATTCAATCAGTGATTCAAACAAATACTAAAATGGGAATGATAACAATGGAACAATCA
CTTGTTGATTTGTATAAAAGAGGACTTATAACAAGAGAAGATGCAATAATGTACGCATCTGACCAGGAACTTATAAACAA
GCTTTTGATATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E4Q3R8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.448

99.145

0.53
  pilT Acinetobacter nosocomialis M2

53.353

97.721

0.521
  pilT Acinetobacter baumannii D1279779

53.353

97.721

0.521
  pilT Acinetobacter baumannii strain A118

53.353

97.721

0.521
  pilT Legionella pneumophila strain Lp02

53.043

98.291

0.521
  pilT Legionella pneumophila strain ERS1305867

53.043

98.291

0.521
  pilT Neisseria meningitidis 8013

52.326

98.006

0.513
  pilT Acinetobacter baylyi ADP1

52.187

97.721

0.51
  pilT Pseudomonas stutzeri DSM 10701

51.895

97.721

0.507
  pilT Pseudomonas aeruginosa PAK

52.836

95.442

0.504
  pilT Neisseria gonorrhoeae MS11

51.603

97.721

0.504
  pilT Vibrio cholerae strain A1552

52.395

95.157

0.499
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.395

95.157

0.499
  pilU Pseudomonas stutzeri DSM 10701

43.658

96.581

0.422
  pilU Acinetobacter baylyi ADP1

41.765

96.866

0.405
  pilU Vibrio cholerae strain A1552

38.348

96.581

0.37

Multiple sequence alignment