Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CALHY_RS04520 Genome accession   NC_014652
Coordinates   934096..935151 (+) Length   351 a.a.
NCBI ID   WP_013402821.1    Uniprot ID   -
Organism   Caldicellulosiruptor hydrothermalis 108     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 929096..940151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CALHY_RS04500 (Calhy_0893) - 929917..930816 (-) 900 WP_013402817.1 hypothetical protein -
  CALHY_RS04505 (Calhy_0894) - 930993..931502 (+) 510 WP_013402818.1 YqeG family HAD IIIA-type phosphatase -
  CALHY_RS04510 (Calhy_0895) aroE 931499..932344 (+) 846 WP_013402819.1 shikimate dehydrogenase -
  CALHY_RS04515 (Calhy_0896) - 932382..934064 (+) 1683 WP_013402820.1 GspE/PulE family protein -
  CALHY_RS04520 (Calhy_0897) pilT 934096..935151 (+) 1056 WP_013402821.1 type IV pilus twitching motility protein PilT Machinery gene
  CALHY_RS04525 (Calhy_0898) - 935168..936379 (+) 1212 WP_013402822.1 type II secretion system F family protein -
  CALHY_RS04530 (Calhy_0899) - 936412..936852 (+) 441 WP_013402823.1 type II secretion system protein -
  CALHY_RS04535 (Calhy_0900) - 936891..937637 (+) 747 WP_238524584.1 A24 family peptidase -
  CALHY_RS04540 (Calhy_0901) - 937663..937935 (+) 273 WP_013402825.1 late competence development ComFB family protein -
  CALHY_RS04545 (Calhy_0902) - 937922..938509 (+) 588 WP_041723110.1 Tfp pilus assembly protein FimT/FimU -
  CALHY_RS04550 (Calhy_0903) - 938506..939012 (+) 507 WP_013402827.1 PilW family protein -
  CALHY_RS04555 (Calhy_0904) pilM 939045..940019 (+) 975 WP_013402828.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39264.53 Da        Isoelectric Point: 7.5734

>NTDB_id=38846 CALHY_RS04520 WP_013402821.1 934096..935151(+) (pilT) [Caldicellulosiruptor hydrothermalis 108]
MNIDEILKEAFLKGASDIHITVGLPPTIRVHGNLIRLGDEKLLPKDIESIVKYIVPADHLEKLYENGEVDFSYSLQGISR
FRVNVFKQRGSYAIAFRIIPQEIPKFENLGLPPVIREFTKLNKGLVLVTGPTGSGKSTTLASLIDIINTERSVHIITLED
PIEYLHKHKSSIVNQREIGSDSKSFAAALRAALREDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGSAKTIDRI
IDVFPPHQQQQVRVQLSTVLQAVVSQQLLTRRDKLGRVVACEVMIATPAIRNLIRENKTYQIQSVIQTSMKNGMITMEHS
LVELYRKGCITKEDVFTYAPDQDIVNKLLQL

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=38846 CALHY_RS04520 WP_013402821.1 934096..935151(+) (pilT) [Caldicellulosiruptor hydrothermalis 108]
ATGAACATTGATGAGATTCTTAAAGAAGCTTTTTTAAAAGGTGCCTCAGATATTCACATAACGGTGGGATTGCCTCCAAC
TATTAGGGTTCATGGAAATTTGATTCGGCTTGGGGATGAGAAACTTTTGCCTAAGGATATAGAGTCAATTGTAAAATACA
TTGTGCCGGCTGATCATCTTGAAAAACTTTATGAGAATGGTGAAGTTGATTTTTCTTATAGCCTACAAGGAATTAGTAGA
TTCAGAGTAAATGTATTCAAGCAAAGGGGTTCATATGCAATTGCTTTTAGAATCATTCCTCAAGAAATTCCAAAATTTGA
GAATTTGGGACTTCCACCTGTTATCAGAGAATTCACAAAGCTTAATAAGGGATTGGTTTTAGTTACTGGTCCAACTGGTT
CAGGAAAATCAACTACACTTGCCTCGCTGATAGATATAATAAATACAGAAAGAAGTGTTCATATAATTACCCTTGAAGAT
CCAATCGAATACTTGCATAAACACAAAAGCAGTATAGTAAATCAAAGAGAAATCGGAAGCGATTCTAAAAGTTTTGCAGC
AGCTCTTCGCGCTGCTTTACGTGAAGACCCGGATGTAATCTTAGTTGGTGAGATGAGAGATTTGGAAACAATCTCGATTG
CTCTTACCGCTGCAGAGACCGGGCACTTAGTATTTTCAACTCTTCACACTATTGGTTCTGCAAAAACAATCGATAGGATA
ATTGATGTTTTTCCACCACATCAGCAACAGCAAGTAAGAGTCCAACTGTCAACTGTACTCCAGGCAGTAGTTTCTCAACA
ATTGCTTACGCGTAGAGATAAACTTGGTAGAGTAGTTGCTTGTGAGGTAATGATTGCAACGCCGGCTATAAGAAATTTGA
TTAGAGAAAACAAAACTTACCAGATTCAATCTGTGATTCAGACAAGTATGAAAAATGGAATGATAACGATGGAGCATTCG
TTAGTAGAGTTGTATCGCAAAGGGTGTATCACAAAAGAAGATGTTTTTACATATGCGCCTGACCAAGACATAGTGAATAA
ATTACTCCAACTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

54.354

94.872

0.516
  pilT Legionella pneumophila strain Lp02

54.354

94.872

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.613

97.151

0.501
  pilT Acinetobacter baumannii strain A118

51.471

96.866

0.499
  pilT Acinetobacter nosocomialis M2

51.471

96.866

0.499
  pilT Acinetobacter baumannii D1279779

51.471

96.866

0.499
  pilT Neisseria meningitidis 8013

52.239

95.442

0.499
  pilT Neisseria gonorrhoeae MS11

51.497

95.157

0.49
  pilT Acinetobacter baylyi ADP1

51.807

94.587

0.49
  pilT Pseudomonas stutzeri DSM 10701

51.351

94.872

0.487
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.595

95.726

0.484
  pilT Vibrio cholerae strain A1552

50.595

95.726

0.484
  pilT Pseudomonas aeruginosa PAK

50.448

95.442

0.481
  pilU Pseudomonas stutzeri DSM 10701

39.601

100

0.396
  pilU Vibrio cholerae strain A1552

36.857

99.715

0.368
  pilU Acinetobacter baylyi ADP1

37.941

96.866

0.368

Multiple sequence alignment