Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FOB24_RS21050 Genome accession   NZ_CP044101
Coordinates   4280113..4281093 (-) Length   326 a.a.
NCBI ID   WP_150345414.1    Uniprot ID   -
Organism   Citrobacter werkmanii strain FDAARGOS_616     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4275113..4286093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOB24_RS21020 (FOB24_21025) - 4275960..4276457 (+) 498 WP_150345413.1 SprT family zinc-dependent metalloprotease -
  FOB24_RS21025 (FOB24_21030) endA 4276552..4277259 (+) 708 WP_085048753.1 deoxyribonuclease I -
  FOB24_RS21030 (FOB24_21035) rsmE 4277334..4278065 (+) 732 WP_038633593.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  FOB24_RS21035 (FOB24_21040) gshB 4278078..4279025 (+) 948 WP_038633598.1 glutathione synthase -
  FOB24_RS21040 (FOB24_21045) - 4279137..4279700 (+) 564 WP_032940453.1 YqgE/AlgH family protein -
  FOB24_RS21045 (FOB24_21050) ruvX 4279700..4280116 (+) 417 WP_038633601.1 Holliday junction resolvase RuvX -
  FOB24_RS21050 (FOB24_21055) pilT 4280113..4281093 (-) 981 WP_150345414.1 type IV pilus twitching motility protein PilT Machinery gene
  FOB24_RS21055 (FOB24_21060) - 4281111..4281815 (+) 705 WP_038633606.1 YggS family pyridoxal phosphate-dependent enzyme -
  FOB24_RS21060 (FOB24_21065) - 4281834..4282400 (+) 567 WP_038633609.1 YggT family protein -
  FOB24_RS21065 (FOB24_21070) yggU 4282397..4282687 (+) 291 WP_003027101.1 DUF167 family protein YggU -
  FOB24_RS21070 (FOB24_21075) - 4282695..4283288 (+) 594 WP_038633614.1 XTP/dITP diphosphatase -
  FOB24_RS21075 (FOB24_21080) hemW 4283281..4284417 (+) 1137 WP_150345415.1 radical SAM family heme chaperone HemW -
  FOB24_RS21080 (FOB24_21085) ansB 4284534..4285580 (-) 1047 WP_038633619.1 L-asparaginase 2 -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35871.15 Da        Isoelectric Point: 6.8183

>NTDB_id=387840 FOB24_RS21050 WP_150345414.1 4280113..4281093(-) (pilT) [Citrobacter werkmanii strain FDAARGOS_616]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGNVEIAPFTTPDVETLLMCWLSEQQQVQWQKQGQLDFAITLTNSRRLRA
SAFVHQQGISLALRLLPLDCPCLDDLQTPAALPELLHSENGLILVTGATGSGKSTTLAAMVAYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAPEKDPVRNQLADSLRAVLSQKLEQDKQGGRVALFELLINTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFTQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=387840 FOB24_RS21050 WP_150345414.1 4280113..4281093(-) (pilT) [Citrobacter werkmanii strain FDAARGOS_616]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
TTGGCGCATACGCGGAAATGTCGAAATCGCGCCGTTTACCACGCCTGACGTAGAGACGTTGCTGATGTGCTGGCTCAGCG
AGCAACAACAGGTACAGTGGCAGAAACAGGGGCAGCTGGATTTTGCCATTACGCTGACGAACTCACGGCGTCTGCGTGCG
AGCGCATTTGTTCATCAGCAGGGAATCTCTCTGGCGCTAAGGCTATTGCCGCTTGATTGCCCATGTTTGGATGATCTCCA
GACTCCCGCCGCCTTACCTGAGCTGCTGCACAGTGAAAATGGATTAATTCTGGTGACCGGCGCTACCGGCAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTGGCATATCTTAATCAGCACGTCGCGGGACATATTTTGACGCTGGAAGATCCCATTGAA
TATCGTTACACCAGCCAGCGTTGTCTCATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTACGCGAAGATCCTGATGTCATTCTGCTGGGCGAGCTGCGCGACGTAGAAACCATTCGTCTGGCGTTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACACACGCGAGGGGCGGCTCAGGCGATTGCGCGGCTGGTGGATACT
TTTCCCGCACCGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCAGTGCTTTCGCAAAAGCTGGAACA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAACTGCTCATCAACACGCCCGCCGTCGGTAACCTGATCCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAACAGACAGGCATGCAGACGTTTACGCAGAGCCTGCAGCAGCGC
CAGGCGCAGGGACGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.121

98.466

0.405
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383
  pilU Vibrio cholerae strain A1552

40

95.092

0.38

Multiple sequence alignment