Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ILYOP_RS01340 Genome accession   NC_014632
Coordinates   286611..287660 (+) Length   349 a.a.
NCBI ID   WP_013386708.1    Uniprot ID   -
Organism   Ilyobacter polytropus DSM 2926     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 281611..292660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ILYOP_RS01310 (Ilyop_0242) - 282326..283060 (+) 735 WP_013386702.1 hypothetical protein -
  ILYOP_RS01315 (Ilyop_0243) - 283213..283410 (+) 198 WP_013386703.1 cysteine-rich CWC family protein -
  ILYOP_RS01320 (Ilyop_0244) - 283421..284257 (+) 837 WP_013386704.1 PHP domain-containing protein -
  ILYOP_RS01325 (Ilyop_0245) - 284254..284673 (+) 420 WP_013386705.1 nucleoside 2-deoxyribosyltransferase -
  ILYOP_RS01330 (Ilyop_0246) rfaD 284747..285745 (+) 999 WP_013386706.1 ADP-glyceromanno-heptose 6-epimerase -
  ILYOP_RS01335 (Ilyop_0247) - 285760..286563 (+) 804 WP_013386707.1 undecaprenyl-diphosphate phosphatase -
  ILYOP_RS01340 (Ilyop_0248) pilT 286611..287660 (+) 1050 WP_013386708.1 type IV pilus twitching motility protein PilT Machinery gene
  ILYOP_RS01345 (Ilyop_0249) - 287671..288879 (+) 1209 WP_013386709.1 type II secretion system F family protein -
  ILYOP_RS01350 (Ilyop_0250) - 288897..290291 (+) 1395 WP_013386710.1 coproporphyrinogen III oxidase -
  ILYOP_RS01355 (Ilyop_0251) - 290317..290865 (+) 549 WP_013386711.1 helix-hairpin-helix domain-containing protein -
  ILYOP_RS01360 (Ilyop_0252) hslO 290982..291869 (+) 888 WP_013386712.1 Hsp33 family molecular chaperone HslO -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38780.68 Da        Isoelectric Point: 6.8367

>NTDB_id=38730 ILYOP_RS01340 WP_013386708.1 286611..287660(+) (pilT) [Ilyobacter polytropus DSM 2926]
MNITDILVAAKDAEASDIHLVAGKPPTFRIHGVLEDMPGCEEKLMPLAVKELAYSILNEDQEKSFEKNKELDFSFGISGV
GRYRVNVHVQRGTIGVSIRSLSTEIPDFSKLGLPEVVRSFTEYENGLILVTGPTGSGKSTTLASLIDKINKEKAHHIITV
EDPIEYLHTHKRALVEQRELKADTESFSTALKYALRQDPDVILIGEMRDLETITAALTAAETGHLVFGTLHTNSAAKTID
RIIDVFPMDQQSQIRIQLSTSLRAVLAQQLVPSTDRKRKVACEIMVGTPAIGNLIRENKTYQIPSMIETGMRHGMISMDA
FLEKLLKQGKITTQEFEKRSSVKKNKIDL

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=38730 ILYOP_RS01340 WP_013386708.1 286611..287660(+) (pilT) [Ilyobacter polytropus DSM 2926]
ATGAATATTACAGACATTCTAGTTGCAGCAAAGGACGCAGAGGCATCTGATATCCATCTTGTGGCAGGAAAACCGCCTAC
ATTCAGAATCCATGGTGTTTTGGAAGATATGCCCGGCTGCGAGGAAAAATTAATGCCTTTGGCTGTAAAAGAACTTGCCT
ACTCAATATTAAATGAGGATCAGGAAAAAAGTTTTGAAAAAAATAAAGAGTTGGATTTTTCCTTTGGTATATCAGGAGTT
GGTAGATACAGGGTAAATGTCCATGTACAGAGAGGTACAATAGGAGTTTCGATAAGATCACTGAGTACAGAGATACCTGA
TTTTTCAAAATTAGGTCTACCTGAAGTGGTAAGAAGTTTCACAGAATATGAAAACGGTCTGATACTGGTAACAGGTCCTA
CTGGAAGTGGTAAATCTACTACACTGGCCTCTTTGATTGATAAAATAAACAAAGAAAAAGCACATCATATAATAACTGTA
GAGGACCCCATAGAATATCTTCATACTCATAAAAGGGCTTTGGTGGAGCAAAGGGAATTAAAGGCCGATACAGAAAGTTT
TTCTACGGCTCTTAAATATGCTCTGAGACAGGATCCAGATGTAATTCTTATAGGTGAGATGAGGGACCTTGAAACTATAA
CAGCTGCCCTTACAGCAGCAGAAACCGGTCACTTGGTATTTGGGACACTGCATACAAACAGTGCAGCAAAGACAATAGAT
AGAATAATAGATGTATTTCCTATGGATCAGCAGTCTCAGATAAGAATACAGCTGTCAACTTCATTAAGAGCGGTATTGGC
TCAGCAACTTGTTCCTTCTACTGACCGCAAGAGAAAAGTTGCCTGTGAAATAATGGTAGGAACCCCGGCAATAGGAAACT
TAATAAGAGAAAATAAGACATATCAGATACCTTCAATGATAGAAACTGGTATGAGACACGGAATGATAAGTATGGACGCT
TTTTTGGAAAAGCTTCTTAAACAAGGTAAAATAACCACACAGGAATTTGAAAAAAGATCCAGTGTTAAGAAAAATAAAAT
TGATCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.326

98.567

0.516
  pilT Pseudomonas stutzeri DSM 10701

49.856

99.427

0.496
  pilT Vibrio cholerae strain A1552

49.854

98.281

0.49
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.854

98.281

0.49
  pilT Pseudomonas aeruginosa PAK

48.991

99.427

0.487
  pilT Acinetobacter baylyi ADP1

50

95.702

0.479
  pilT Acinetobacter baumannii strain A118

49.702

96.275

0.479
  pilT Acinetobacter nosocomialis M2

49.702

96.275

0.479
  pilT Acinetobacter baumannii D1279779

49.702

96.275

0.479
  pilT Legionella pneumophila strain ERS1305867

49.401

95.702

0.473
  pilT Legionella pneumophila strain Lp02

49.401

95.702

0.473
  pilT Neisseria gonorrhoeae MS11

47.11

99.14

0.467
  pilT Neisseria meningitidis 8013

47.11

99.14

0.467
  pilU Acinetobacter baylyi ADP1

38.617

99.427

0.384
  pilU Pseudomonas stutzeri DSM 10701

40.994

92.264

0.378
  pilU Vibrio cholerae strain A1552

39.009

92.55

0.361

Multiple sequence alignment