Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   STAUR_RS31770 Genome accession   NC_014623
Coordinates   7911013..7912122 (-) Length   369 a.a.
NCBI ID   WP_013377318.1    Uniprot ID   E3FK31
Organism   Stigmatella aurantiaca DW4/3-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7906013..7917122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STAUR_RS31755 (STAUR_6454) - 7906707..7908071 (-) 1365 WP_002614993.1 sigma-54 dependent transcriptional regulator -
  STAUR_RS31760 (STAUR_6455) - 7908082..7909674 (-) 1593 WP_013377316.1 ATP-binding protein -
  STAUR_RS31765 (STAUR_6456) - 7909718..7910959 (-) 1242 WP_013377317.1 type II secretion system F family protein -
  STAUR_RS31770 (STAUR_6457) pilT 7911013..7912122 (-) 1110 WP_013377318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  STAUR_RS31775 (STAUR_6458) pilB 7912174..7913877 (-) 1704 WP_002614985.1 type IV-A pilus assembly ATPase PilB -
  STAUR_RS31780 (STAUR_6459) - 7914084..7915007 (-) 924 WP_013377319.1 bifunctional riboflavin kinase/FAD synthetase -
  STAUR_RS31785 (STAUR_6460) - 7915087..7915707 (+) 621 WP_013377320.1 tRNA (guanine-N(7)-)-methyltransferase -
  STAUR_RS31790 (STAUR_6461) - 7915791..7916837 (+) 1047 WP_013377321.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40510.70 Da        Isoelectric Point: 8.2898

>NTDB_id=38701 STAUR_RS31770 WP_013377318.1 7911013..7912122(-) (pilT) [Stigmatella aurantiaca DW4/3-1]
MANLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAPLTPVETKQLCYSILTDAQKHKFEEDNELDLSFGVKGLS
RFRANIFMQRGAVAAAFRTIPFKILTFQELGLPQVVADLIKRPRGLILVTGPTGSGKSTTLASMIDKINSERHEHIMTIE
DPIEYLHPHKNCLVNQREVGADTRNFKTALRYILRQDPDVVLVGELRDLETIEAALTIAETGHTCYATLHTNSAVQTINR
VLDVFPPYQQPQVRAQMSFVLEGVMSQALVAKQGSPGRVLALEVMIPTPAIRNLIREDKVHQVYSSMQVGQAKYGMQTFN
QALAALLARRLISQEEAMGRSSDPEELRNILAGSAPGGVQRPGGGAPGR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=38701 STAUR_RS31770 WP_013377318.1 7911013..7912122(-) (pilT) [Stigmatella aurantiaca DW4/3-1]
GTGGCCAACCTGCACCAGCTCCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACATCACCACTGGCTCTCCGCC
GCAGCTCCGGGTGGACGGCGAACTGGTGCCACTGAAGACGGCGCCCCTCACGCCGGTGGAGACCAAGCAGCTTTGCTACT
CCATCCTCACGGATGCCCAGAAGCACAAGTTCGAAGAGGACAACGAGCTGGACTTGTCCTTCGGTGTGAAGGGGCTGTCG
CGCTTCCGCGCCAACATCTTCATGCAGCGCGGCGCGGTGGCCGCGGCCTTCCGGACCATTCCCTTCAAGATCCTCACCTT
CCAGGAGCTGGGCTTGCCCCAGGTGGTGGCGGACCTCATCAAGCGTCCGCGTGGGCTCATCCTCGTCACGGGCCCCACCG
GCTCGGGCAAGTCCACCACGCTGGCGTCGATGATCGACAAGATCAACAGCGAGCGTCATGAGCACATCATGACGATCGAG
GATCCGATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTCAATCAGCGCGAGGTGGGCGCGGACACCCGTAACTTCAA
GACGGCGCTGCGCTACATCCTGCGTCAGGATCCGGACGTGGTGCTGGTGGGTGAGTTGCGCGACCTGGAGACCATCGAGG
CCGCGCTCACCATCGCCGAGACGGGCCACACCTGTTACGCCACCCTGCACACCAACAGCGCGGTGCAGACCATCAACCGC
GTGCTGGACGTGTTCCCGCCGTACCAGCAGCCGCAGGTGCGCGCGCAGATGTCCTTCGTGCTCGAAGGCGTGATGAGCCA
GGCGCTGGTCGCCAAGCAGGGCAGTCCCGGCCGCGTGCTGGCCCTGGAGGTCATGATCCCCACGCCCGCCATCCGGAACC
TCATCCGCGAAGACAAGGTGCACCAGGTCTACTCTTCCATGCAGGTGGGACAGGCCAAGTACGGCATGCAGACCTTCAAT
CAGGCCCTGGCGGCGCTCCTGGCGCGCCGGCTCATCAGCCAGGAAGAGGCCATGGGCCGCTCCAGCGATCCCGAGGAGTT
GCGCAACATCCTGGCCGGCAGCGCCCCGGGCGGCGTGCAGCGGCCAGGCGGCGGAGCCCCTGGCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E3FK31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.043

93.496

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.645

0.493
  pilT Pseudomonas stutzeri DSM 10701

52.464

93.496

0.491
  pilT Acinetobacter baumannii D1279779

52.464

93.496

0.491
  pilT Acinetobacter baumannii strain A118

52.464

93.496

0.491
  pilT Acinetobacter nosocomialis M2

52.174

93.496

0.488
  pilT Legionella pneumophila strain Lp02

53.731

90.786

0.488
  pilT Legionella pneumophila strain ERS1305867

53.731

90.786

0.488
  pilT Acinetobacter baylyi ADP1

51.884

93.496

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.817

91.599

0.447
  pilT Vibrio cholerae strain A1552

48.817

91.599

0.447
  pilT Neisseria meningitidis 8013

45.161

92.412

0.417
  pilT Neisseria gonorrhoeae MS11

44.868

92.412

0.415
  pilU Vibrio cholerae strain A1552

42.816

94.309

0.404
  pilU Pseudomonas stutzeri DSM 10701

41.739

93.496

0.39
  pilU Acinetobacter baylyi ADP1

39.481

94.038

0.371

Multiple sequence alignment