Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ENTCL_RS04035 Genome accession   NC_014618
Coordinates   838504..839484 (+) Length   326 a.a.
NCBI ID   WP_013364832.1    Uniprot ID   -
Organism   [Enterobacter] lignolyticus SCF1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 833504..844484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENTCL_RS04005 (Entcl_0799) ansB 834079..835125 (+) 1047 WP_013364826.1 L-asparaginase 2 -
  ENTCL_RS04010 (Entcl_0800) hemW 835173..836306 (-) 1134 WP_013364827.1 radical SAM family heme chaperone HemW -
  ENTCL_RS04015 (Entcl_0801) - 836310..836903 (-) 594 WP_013364828.1 XTP/dITP diphosphatase -
  ENTCL_RS04020 (Entcl_0802) yggU 836912..837202 (-) 291 WP_013364829.1 DUF167 family protein YggU -
  ENTCL_RS04025 (Entcl_0803) - 837199..837765 (-) 567 WP_013364830.1 YggT family protein -
  ENTCL_RS04030 (Entcl_0804) - 837784..838488 (-) 705 WP_044612060.1 YggS family pyridoxal phosphate-dependent enzyme -
  ENTCL_RS04035 (Entcl_0805) pilT 838504..839484 (+) 981 WP_013364832.1 type IV pilus twitching motility protein PilT Machinery gene
  ENTCL_RS04040 (Entcl_0806) - 839895..840548 (+) 654 WP_013364833.1 LuxR C-terminal-related transcriptional regulator -
  ENTCL_RS04045 (Entcl_0807) ruvX 840589..841005 (-) 417 WP_013364834.1 Holliday junction resolvase RuvX -
  ENTCL_RS04050 (Entcl_0808) - 841005..841568 (-) 564 WP_044611886.1 YqgE/AlgH family protein -
  ENTCL_RS04055 (Entcl_0809) gshB 841667..842614 (-) 948 WP_013364836.1 glutathione synthase -
  ENTCL_RS04060 (Entcl_0810) rsmE 842627..843358 (-) 732 WP_013364837.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ENTCL_RS04065 (Entcl_0811) endA 843404..844111 (-) 708 WP_013364838.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35088.20 Da        Isoelectric Point: 7.0397

>NTDB_id=38639 ENTCL_RS04035 WP_013364832.1 838504..839484(+) (pilT) [[Enterobacter] lignolyticus SCF1]
MDMQEIVSLSVKHNVSDLHLCSCMQARWRREGRLEPAPFPSPDLPMLLDGWLNAAQQRQWREDRQVDFAVDAGGGQRLRG
NASVHCRGVSVVLRLLPVSCPQLSTLGAPAAIPSLLDAESGIILVTGATGSGKSTTLAAMVDSLNQQRDGHILTLEDPVE
FVHHSARCLIEQREVGLHCASYAAALRSALRQDPDVLLLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAVERLIDS
FPAGEKETVRSQLAGSLCAVLAQKLLPGRLGGRVALYELLINTAATASLIREGKTHQLAGVMQTGQQAGMQTYAQSHQQR
IAEGKM

Nucleotide


Download         Length: 981 bp        

>NTDB_id=38639 ENTCL_RS04035 WP_013364832.1 838504..839484(+) (pilT) [[Enterobacter] lignolyticus SCF1]
ATGGATATGCAGGAAATTGTGTCCCTTAGTGTAAAGCATAACGTTTCGGATCTACACCTGTGTAGTTGCATGCAGGCGCG
CTGGCGGCGAGAAGGGCGTCTCGAGCCCGCGCCTTTTCCGTCGCCGGATCTCCCTATGCTGCTCGACGGCTGGCTTAATG
CGGCGCAGCAGCGGCAGTGGCGCGAGGATCGCCAGGTGGATTTTGCCGTGGATGCCGGAGGCGGTCAGCGCCTTCGCGGC
AATGCGTCCGTGCACTGTCGGGGCGTTTCGGTGGTTCTGCGCCTGCTGCCGGTCTCCTGCCCGCAGCTTTCGACGCTGGG
GGCGCCTGCGGCGATCCCTTCGCTGCTCGACGCCGAGAGCGGGATTATTCTGGTGACCGGCGCGACCGGCAGCGGCAAGT
CCACGACGCTTGCCGCGATGGTGGATTCTCTTAACCAGCAGCGGGACGGGCATATTCTCACGCTTGAAGATCCGGTGGAG
TTTGTCCATCACAGCGCGCGGTGTCTGATTGAGCAACGGGAGGTGGGTTTGCACTGCGCGTCCTATGCCGCCGCGCTGCG
AAGCGCATTGCGCCAGGACCCGGATGTTCTGCTGCTGGGAGAGCTGCGTGATGCTGAAACTATCCGTCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTACTGGCGACCCTGCACACCCGCGGCGCGGCGCAGGCCGTGGAGCGGCTCATTGACAGC
TTCCCTGCCGGGGAAAAAGAGACCGTCCGCAGCCAGCTTGCCGGTAGCCTGTGCGCGGTGCTGGCGCAGAAGCTGCTGCC
CGGCAGGCTGGGGGGAAGGGTGGCGCTGTATGAGCTGCTGATCAACACCGCGGCGACCGCTAGCTTGATTCGCGAGGGGA
AAACGCACCAGCTGGCAGGCGTGATGCAGACAGGGCAGCAGGCCGGGATGCAAACCTACGCCCAAAGCCATCAGCAGCGG
ATTGCCGAGGGAAAAATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

100

0.503
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.17

97.546

0.46
  pilT Legionella pneumophila strain Lp02

47.17

97.546

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.987

96.933

0.426
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.066

100

0.386
  pilU Acinetobacter baylyi ADP1

37.072

98.466

0.365

Multiple sequence alignment