Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0U60_RS13175 Genome accession   NZ_CP043494
Coordinates   3221056..3222165 (+) Length   369 a.a.
NCBI ID   WP_395818826.1    Uniprot ID   -
Organism   Archangium minus strain Cbm 6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3216056..3227165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0U60_RS13155 (F0U60_13280) - 3216347..3217402 (-) 1056 WP_395818814.1 diguanylate cyclase -
  F0U60_RS13160 (F0U60_13285) - 3217512..3218132 (-) 621 WP_395818817.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  F0U60_RS13165 (F0U60_13290) - 3218225..3219151 (+) 927 WP_395818820.1 bifunctional riboflavin kinase/FAD synthetase -
  F0U60_RS13170 (F0U60_13295) pilB 3219306..3221009 (+) 1704 WP_395818823.1 type IV-A pilus assembly ATPase PilB -
  F0U60_RS13175 (F0U60_13300) pilT 3221056..3222165 (+) 1110 WP_395818826.1 type IV pilus twitching motility protein PilT Machinery gene
  F0U60_RS13180 (F0U60_13305) - 3222217..3223476 (+) 1260 WP_395818829.1 type II secretion system F family protein -
  F0U60_RS13185 (F0U60_13310) - 3223490..3225091 (+) 1602 WP_395818832.1 nitrogen regulation protein NR(II) -
  F0U60_RS13190 (F0U60_13315) - 3225114..3226508 (+) 1395 WP_395818836.1 sigma-54-dependent transcriptional regulator -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40509.74 Da        Isoelectric Point: 8.2875

>NTDB_id=384592 F0U60_RS13175 WP_395818826.1 3221056..3222165(+) (pilT) [Archangium minus strain Cbm 6]
MNLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAQLTPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLSR
FRANIYMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKKPRGLILVTGPTGSGKSTTLASMIDKINTDRHEHIMTIED
PIEYLHPHKNCLVNQREVGADTRNFKTALKYILRQDPDVVLVGELRDLETIEAALVIAETGHTCYATLHTNSAVQTINRV
LDVFPPYQQPQVRAQLSFVLEGVMSQALIAKQGSPGRVLALEVMIPNSAIRNLIREDKIHQVYSSMQVGQAKFGMQTFNQ
ALAALLMRRLISQEEAFGRSSDPDELRNLLAGAGPGGAPGQRPAGPGAR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=384592 F0U60_RS13175 WP_395818826.1 3221056..3222165(+) (pilT) [Archangium minus strain Cbm 6]
GTGAATCTGCATCAGCTGCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACATCACCACCGGCTCCCCGCCCCA
GCTCCGCGTGGACGGCGAGCTGGTGCCGCTCAAGACGGCCCAGCTCACCCCGGTGGAGACCAAGCAGCTCTGCTACTCCA
TCCTCACGGACGCTCAGAAGCACAAGTTCGAGGAAGAGAACGAGCTCGACCTGTCCTTCGGCGTGAAGGGCCTCTCGCGC
TTCCGCGCCAACATCTACATGCAGCGCGGCGCGGTGGCCGGGGCCTTCCGGACCATTCCCTTCAAGATCCTCACCTTCCA
GGAGCTCGGTCTGCCTCCGGTGGTGGCCGAGCTGGTGAAGAAGCCGCGTGGCCTCATCCTCGTGACGGGTCCCACGGGCT
CGGGCAAGAGCACCACGCTGGCCTCGATGATCGACAAGATCAACACCGACCGTCATGAGCACATCATGACGATCGAGGAC
CCCATCGAGTACCTGCACCCGCACAAGAACTGCCTGGTGAACCAGCGCGAGGTGGGAGCCGACACGCGCAACTTCAAGAC
GGCGCTCAAGTACATCCTCCGCCAGGACCCGGACGTGGTGCTGGTGGGTGAGTTGCGCGACCTGGAGACCATCGAAGCGG
CGCTCGTGATCGCCGAGACGGGCCACACCTGCTACGCCACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGCGTG
CTGGACGTGTTCCCCCCGTACCAGCAGCCGCAGGTGCGCGCCCAGCTGTCATTCGTGCTGGAAGGCGTGATGAGTCAGGC
GCTCATCGCCAAGCAGGGCTCTCCGGGCCGCGTGCTGGCGCTGGAGGTGATGATCCCCAACTCCGCTATCCGCAACCTCA
TCCGTGAGGACAAGATCCACCAGGTCTACTCGTCGATGCAGGTGGGTCAGGCCAAGTTCGGCATGCAGACCTTCAACCAG
GCCTTGGCTGCGCTGCTGATGCGGCGGCTCATCAGCCAGGAAGAGGCCTTTGGACGCTCGAGCGATCCGGACGAGCTCCG
CAATCTCCTGGCGGGTGCTGGGCCTGGCGGCGCGCCTGGTCAGCGGCCAGCCGGTCCTGGTGCACGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.457

99.729

0.493
  pilT Legionella pneumophila strain Lp02

53.392

91.87

0.491
  pilT Legionella pneumophila strain ERS1305867

53.392

91.87

0.491
  pilT Pseudomonas aeruginosa PAK

51.576

94.58

0.488
  pilT Pseudomonas stutzeri DSM 10701

50.289

93.767

0.472
  pilT Acinetobacter baumannii strain A118

50

93.767

0.469
  pilT Acinetobacter nosocomialis M2

50

93.767

0.469
  pilT Acinetobacter baumannii D1279779

50

93.767

0.469
  pilT Acinetobacter baylyi ADP1

49.711

93.767

0.466
  pilT Vibrio cholerae strain A1552

47.463

90.786

0.431
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.463

90.786

0.431
  pilT Neisseria meningitidis 8013

43.931

93.767

0.412
  pilT Neisseria gonorrhoeae MS11

43.931

93.767

0.412
  pilU Vibrio cholerae strain A1552

42.286

94.851

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.634

94.038

0.382
  pilU Acinetobacter baylyi ADP1

40.517

94.309

0.382

Multiple sequence alignment