Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0U62_RS40810 Genome accession   NZ_CP043492
Coordinates   9959060..9960169 (-) Length   369 a.a.
NCBI ID   WP_395851365.1    Uniprot ID   -
Organism   Cystobacter fuscus strain Cbf 8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9954060..9965169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0U62_RS40795 (F0U62_40860) - 9954727..9956115 (-) 1389 WP_395851362.1 sigma-54-dependent transcriptional regulator -
  F0U62_RS40800 (F0U62_40865) - 9956138..9957739 (-) 1602 WP_395851363.1 ATP-binding protein -
  F0U62_RS40805 (F0U62_40870) - 9957756..9959009 (-) 1254 WP_395851364.1 type II secretion system F family protein -
  F0U62_RS40810 (F0U62_40875) pilT 9959060..9960169 (-) 1110 WP_395851365.1 type IV pilus twitching motility protein PilT Machinery gene
  F0U62_RS40815 (F0U62_40880) pilB 9960213..9961916 (-) 1704 WP_395851366.1 type IV-A pilus assembly ATPase PilB -
  F0U62_RS40820 (F0U62_40885) - 9962101..9963027 (-) 927 WP_395851367.1 bifunctional riboflavin kinase/FAD synthetase -
  F0U62_RS40825 (F0U62_40890) - 9963114..9963734 (+) 621 WP_395851368.1 tRNA (guanine(46)-N(7))-methyltransferase TrmB -
  F0U62_RS40830 (F0U62_40895) - 9963893..9964966 (+) 1074 WP_395851369.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40471.64 Da        Isoelectric Point: 8.2829

>NTDB_id=384573 F0U62_RS40810 WP_395851365.1 9959060..9960169(-) (pilT) [Cystobacter fuscus strain Cbf 8]
MNLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAQLSPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLSR
FRANIYMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKRPRGLILVTGPTGSGKSTTLASMVDKINTDRHEHIMTIED
PIEYLHPHKNCLVNQREVGADTRSFKTALKYILRQDPDVVLVGELRDLETIEAALVIAETGHTCYATLHTNSAVQTINRV
LDVFPPYQQPQVRAQLSFVLEGVMSQSLIAKAGSPGRVLALEVMIPNPAIRNLIREDKIHQVYSSMQVGQAKYGMQTFNQ
ALAALLARRLISQDEAFGRSSDPDELRNILAGGTAAGMPGQRPAGPAGR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=384573 F0U62_RS40810 WP_395851365.1 9959060..9960169(-) (pilT) [Cystobacter fuscus strain Cbf 8]
GTGAATCTGCATCAGCTGCTCAAGGCGATGGTCGAGAAGGGCGCTTCCGACCTCCACATCACCACCGGCTCCCCGCCCCA
GCTGCGCGTGGACGGCGAGCTGGTGCCGCTCAAGACGGCCCAGCTGTCGCCGGTGGAGACCAAGCAGCTCTGCTACTCCA
TCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGCTGGACCTCTCCTTCGGCGTGAAGGGGTTGTCGCGC
TTTCGCGCCAACATCTACATGCAGCGTGGCGCGGTGGCGGGGGCGTTCCGCACCATCCCCTTCAAGATCCTCACCTTCCA
GGAGCTGGGTCTGCCCCCGGTGGTGGCCGAGCTCGTCAAGCGCCCGCGCGGCCTCATCCTGGTGACGGGTCCCACCGGCT
CGGGCAAGTCCACGACGCTCGCGTCGATGGTCGACAAGATCAACACCGACCGTCATGAGCACATCATGACGATCGAGGAT
CCGATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTCAACCAGCGCGAGGTGGGCGCCGACACGCGCAGCTTCAAGAC
GGCGCTCAAGTACATCCTGCGCCAGGATCCGGACGTGGTGCTGGTGGGCGAGCTGCGCGACCTGGAGACGATCGAGGCGG
CGCTCGTCATCGCCGAGACGGGCCACACCTGCTACGCCACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGGGTG
CTGGACGTGTTCCCTCCCTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCGTTCGTGCTGGAAGGCGTGATGAGCCAGTC
GCTCATCGCCAAGGCGGGCTCTCCGGGCCGCGTGCTGGCGCTGGAGGTCATGATTCCCAACCCCGCCATCCGCAACCTCA
TCCGCGAGGACAAGATCCATCAGGTCTACTCCTCGATGCAGGTGGGCCAGGCGAAGTACGGCATGCAGACCTTCAACCAG
GCCCTGGCGGCGCTGCTGGCGCGGCGCCTCATCAGCCAGGACGAGGCGTTCGGTCGTTCCTCGGATCCGGACGAGCTGCG
CAACATCCTCGCGGGCGGCACCGCCGCCGGCATGCCTGGTCAGCGGCCCGCCGGCCCCGCGGGGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

99.729

0.499
  pilT Legionella pneumophila strain Lp02

53.687

91.87

0.493
  pilT Legionella pneumophila strain ERS1305867

53.687

91.87

0.493
  pilT Pseudomonas aeruginosa PAK

51.862

94.58

0.491
  pilT Pseudomonas stutzeri DSM 10701

51.156

93.767

0.48
  pilT Acinetobacter baumannii strain A118

50.578

93.767

0.474
  pilT Acinetobacter baumannii D1279779

50.578

93.767

0.474
  pilT Acinetobacter baylyi ADP1

50.289

93.767

0.472
  pilT Acinetobacter nosocomialis M2

50.289

93.767

0.472
  pilT Vibrio cholerae strain A1552

48.358

90.786

0.439
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.358

90.786

0.439
  pilT Neisseria meningitidis 8013

44.22

93.767

0.415
  pilT Neisseria gonorrhoeae MS11

44.22

93.767

0.415
  pilU Vibrio cholerae strain A1552

43.143

94.851

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.395

95.935

0.388
  pilU Acinetobacter baylyi ADP1

40.805

94.309

0.385

Multiple sequence alignment