Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0U63_RS39405 Genome accession   NZ_CP043491
Coordinates   9609677..9610786 (-) Length   369 a.a.
NCBI ID   WP_395838473.1    Uniprot ID   -
Organism   Cystobacter fuscus strain Cbf 10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 9604677..9615786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0U63_RS39390 (F0U63_39535) - 9605339..9606727 (-) 1389 WP_395838467.1 sigma-54-dependent transcriptional regulator -
  F0U63_RS39395 (F0U63_39540) - 9606752..9608353 (-) 1602 WP_395838469.1 nitrogen regulation protein NR(II) -
  F0U63_RS39400 (F0U63_39545) - 9608370..9609626 (-) 1257 WP_395838471.1 type II secretion system F family protein -
  F0U63_RS39405 (F0U63_39550) pilT 9609677..9610786 (-) 1110 WP_395838473.1 type IV pilus twitching motility protein PilT Machinery gene
  F0U63_RS39410 (F0U63_39555) pilB 9610830..9612533 (-) 1704 WP_395838475.1 type IV-A pilus assembly ATPase PilB -
  F0U63_RS39415 (F0U63_39560) - 9612716..9613642 (-) 927 WP_395838477.1 bifunctional riboflavin kinase/FAD synthetase -
  F0U63_RS39420 (F0U63_39565) trmB 9613733..9614353 (+) 621 WP_395838479.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  F0U63_RS39425 (F0U63_39570) - 9614511..9615584 (+) 1074 WP_395838481.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40485.67 Da        Isoelectric Point: 8.2829

>NTDB_id=384559 F0U63_RS39405 WP_395838473.1 9609677..9610786(-) (pilT) [Cystobacter fuscus strain Cbf 10]
MNLHQLLKAMVEKGASDLHITTGSPPQLRVDGELVPLKTAQLSPVETKQLCYSILTDAQKHKFEEENELDLSFGVKGLSR
FRANIYMQRGAVAGAFRTIPFKILTFQELGLPPVVAELVKRPRGLILVTGPTGSGKSTTLASMIDKINTERHEHIMTIED
PIEYLHPHKNCLVNQREVGADTRSFKTALKYILRQDPDVVLVGELRDLETIEAALVIAETGHTCYATLHTNSAVQTINRV
LDVFPPYQQPQVRAQLSFVLEGVMSQSLIAKAGSPGRVLALEVMVPNPAIRNLIREDKIHQVYSSMQVGQAKYGMQTFNQ
ALAALLARRLISQDEAFGRSSDPDELRNILAGGTAAGMPGQRPAGPAGR

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=384559 F0U63_RS39405 WP_395838473.1 9609677..9610786(-) (pilT) [Cystobacter fuscus strain Cbf 10]
GTGAATCTGCATCAGCTGCTCAAGGCGATGGTCGAGAAGGGTGCTTCCGACCTCCACATCACCACGGGCTCCCCGCCCCA
GTTGCGCGTGGACGGCGAACTGGTGCCGCTCAAGACGGCCCAGCTGTCGCCGGTGGAGACCAAGCAGCTCTGCTACTCCA
TCCTCACCGACGCCCAGAAGCACAAGTTCGAGGAGGAGAACGAGTTGGACCTCTCCTTCGGCGTGAAGGGGTTGTCGCGC
TTTCGCGCCAACATCTACATGCAGCGCGGCGCGGTGGCGGGGGCGTTCCGCACCATTCCCTTCAAGATCCTCACCTTCCA
GGAGCTGGGGCTGCCCCCGGTGGTGGCCGAGCTGGTCAAGCGTCCGCGCGGCCTCATCCTGGTGACGGGTCCCACGGGCT
CGGGCAAGTCCACGACACTCGCGTCGATGATCGACAAGATCAACACCGAGCGTCATGAGCACATCATGACGATCGAGGAT
CCGATCGAGTACCTGCACCCGCACAAGAACTGCCTCGTCAACCAGCGCGAGGTGGGCGCGGACACGCGCAGCTTCAAGAC
GGCGCTCAAGTACATCCTGCGTCAGGATCCAGACGTGGTGCTGGTGGGCGAGTTGCGCGACCTGGAGACGATCGAGGCGG
CGCTGGTCATCGCCGAGACGGGTCACACCTGCTACGCCACGCTGCACACCAACAGCGCGGTGCAGACCATCAACCGCGTG
CTGGACGTGTTCCCGCCCTACCAGCAGCCCCAGGTGCGCGCCCAGCTGTCGTTCGTGCTGGAAGGCGTGATGAGCCAGTC
GCTCATCGCCAAGGCGGGCTCTCCGGGCCGCGTGCTGGCGCTGGAGGTCATGGTGCCCAACCCCGCCATCCGCAACCTCA
TCCGCGAGGACAAGATCCATCAGGTCTACTCGTCGATGCAGGTGGGCCAGGCCAAGTACGGCATGCAGACCTTCAACCAG
GCCCTGGCGGCGCTGTTGGCGCGGCGCCTCATCAGCCAGGACGAGGCGTTCGGCCGTTCCTCGGATCCGGACGAGCTGCG
CAACATCCTCGCGGGCGGCACCGCCGCTGGCATGCCTGGTCAGCGGCCCGCCGGCCCCGCGGGGCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.272

99.729

0.501
  pilT Legionella pneumophila strain Lp02

53.982

91.87

0.496
  pilT Legionella pneumophila strain ERS1305867

53.982

91.87

0.496
  pilT Pseudomonas aeruginosa PAK

51.576

94.58

0.488
  pilT Pseudomonas stutzeri DSM 10701

50.867

93.767

0.477
  pilT Acinetobacter baumannii strain A118

50.289

93.767

0.472
  pilT Acinetobacter baumannii D1279779

50.289

93.767

0.472
  pilT Acinetobacter baylyi ADP1

50

93.767

0.469
  pilT Acinetobacter nosocomialis M2

50

93.767

0.469
  pilT Vibrio cholerae strain A1552

48.06

90.786

0.436
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.06

90.786

0.436
  pilT Neisseria meningitidis 8013

44.22

93.767

0.415
  pilT Neisseria gonorrhoeae MS11

44.22

93.767

0.415
  pilU Vibrio cholerae strain A1552

43.143

94.851

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.395

95.935

0.388
  pilU Acinetobacter baylyi ADP1

40.805

94.309

0.385

Multiple sequence alignment