Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SAUT_RS06915 Genome accession   NC_014506
Coordinates   1350303..1351400 (+) Length   365 a.a.
NCBI ID   WP_013327167.1    Uniprot ID   E0UTX0
Organism   Sulfurimonas autotrophica DSM 16294     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1345303..1356400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAUT_RS06875 (Saut_1359) - 1345352..1346059 (-) 708 WP_013327159.1 sulfite exporter TauE/SafE family protein -
  SAUT_RS06880 (Saut_1360) - 1346059..1346562 (-) 504 WP_013327160.1 hypothetical protein -
  SAUT_RS06885 (Saut_1361) - 1346643..1347119 (-) 477 WP_013327161.1 HD domain-containing protein -
  SAUT_RS06890 (Saut_1362) - 1347116..1347943 (-) 828 WP_013327162.1 tyrosine-type recombinase/integrase -
  SAUT_RS06895 (Saut_1363) - 1347944..1348939 (-) 996 WP_013327163.1 hypothetical protein -
  SAUT_RS06900 (Saut_1364) - 1348943..1349539 (-) 597 WP_013327164.1 hypothetical protein -
  SAUT_RS06905 (Saut_1365) gatC 1349670..1349960 (+) 291 WP_013327165.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  SAUT_RS06910 (Saut_1366) - 1349965..1350306 (+) 342 WP_013327166.1 arsenate reductase family protein -
  SAUT_RS06915 (Saut_1367) pilT 1350303..1351400 (+) 1098 WP_013327167.1 type IV pilus twitching motility protein PilT Machinery gene
  SAUT_RS06920 (Saut_1368) - 1351397..1352389 (-) 993 WP_013327168.1 SAM-dependent methyltransferase -
  SAUT_RS06925 (Saut_1369) - 1352390..1353622 (-) 1233 WP_013327169.1 TolC family protein -
  SAUT_RS06930 (Saut_1370) - 1353579..1354760 (-) 1182 WP_013327170.1 acetylornithine transaminase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40747.74 Da        Isoelectric Point: 6.0645

>NTDB_id=38396 SAUT_RS06915 WP_013327167.1 1350303..1351400(+) (pilT) [Sulfurimonas autotrophica DSM 16294]
MSEEVAAQSSSQKMDIKKLLKSVLAFKSSDLHLVVGSEPQIRIDKELRALNLPVLTANDVEEMAYSLIEDKQKKVFEEHN
ELDFSFELKDIGRFRANFYRTIHGIACAFRMIPIEIPTLDEYGNPPIFKELVKKEKGLILVTGPTGSGKSTTLASMLHEI
NMTERRHIITIEDPVEFVHKNIKSLFSQRDVGSNTESFATALKYSLRQDPDIILIGEMRDAETIGAALTAAETGHLVFGT
LHTNSAPGTINRIIDVFDGEEQAQVRAQLASSLVAVVSQTLIPRVGSGKVATQEILITNPAVQNQIREDKVHQIYSQMQL
NQQETNMTTQTQQLIELLQKKVISKENAIKNSNRPEELLKMIGAL

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=38396 SAUT_RS06915 WP_013327167.1 1350303..1351400(+) (pilT) [Sulfurimonas autotrophica DSM 16294]
ATGAGTGAAGAAGTAGCAGCACAGAGTTCGTCTCAAAAAATGGATATAAAAAAACTGTTAAAAAGTGTTTTGGCATTTAA
GTCTTCTGATTTACACCTGGTTGTCGGTAGTGAACCGCAGATTAGAATAGACAAAGAGTTGCGTGCTTTGAACCTTCCTG
TATTAACTGCTAATGATGTTGAAGAGATGGCATATTCTTTGATCGAGGACAAGCAAAAGAAAGTGTTTGAAGAACACAAC
GAGTTAGATTTCTCATTTGAACTTAAAGATATTGGCCGTTTTCGTGCAAACTTTTATAGAACTATTCACGGAATTGCCTG
TGCTTTTCGTATGATTCCTATTGAGATTCCAACACTTGACGAATATGGTAATCCTCCGATTTTTAAAGAGCTTGTAAAAA
AGGAAAAAGGGCTTATTTTAGTGACCGGCCCTACGGGTTCCGGTAAATCAACAACACTTGCATCAATGCTTCATGAAATA
AATATGACAGAACGCCGCCATATTATCACGATTGAAGATCCGGTGGAGTTCGTTCATAAAAATATAAAATCTCTCTTCTC
TCAACGTGATGTAGGAAGTAATACAGAATCATTTGCCACAGCACTCAAATATTCACTTCGTCAAGATCCCGACATCATTC
TCATCGGGGAGATGCGTGATGCTGAAACCATTGGTGCCGCATTAACAGCAGCCGAAACAGGTCACTTGGTTTTTGGAACC
CTGCATACAAACTCTGCACCTGGAACTATTAACCGTATTATTGATGTATTTGACGGCGAAGAACAAGCTCAAGTTAGAGC
ACAGTTGGCCTCTTCATTAGTAGCTGTAGTTTCCCAAACACTTATTCCAAGAGTGGGAAGCGGTAAAGTTGCTACTCAAG
AAATTCTCATTACAAATCCGGCTGTGCAAAATCAGATTAGAGAAGACAAAGTCCATCAAATTTATTCACAAATGCAGCTG
AATCAACAAGAAACGAATATGACAACGCAAACACAACAGCTTATAGAACTGCTTCAGAAAAAAGTTATTTCAAAAGAAAA
TGCTATAAAAAACTCCAATAGACCTGAGGAATTACTTAAAATGATAGGGGCTCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(19-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0UTX0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

52.836

91.781

0.485
  pilT Legionella pneumophila strain ERS1305867

52.836

91.781

0.485
  pilT Pseudomonas aeruginosa PAK

51.304

94.521

0.485
  pilT Acinetobacter baumannii strain A118

50.867

94.795

0.482
  pilT Acinetobacter baumannii D1279779

50.867

94.795

0.482
  pilT Acinetobacter nosocomialis M2

50.867

94.795

0.482
  pilT Acinetobacter baylyi ADP1

50.725

94.521

0.479
  pilT Pseudomonas stutzeri DSM 10701

50.145

94.521

0.474
  pilT Neisseria gonorrhoeae MS11

49.711

94.795

0.471
  pilT Neisseria meningitidis 8013

49.422

94.795

0.468
  pilT Vibrio cholerae strain A1552

51.051

91.233

0.466
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.051

91.233

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.875

96.438

0.452
  pilU Acinetobacter baylyi ADP1

38.617

95.068

0.367
  pilU Vibrio cholerae strain A1552

37.5

96.438

0.362

Multiple sequence alignment