Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D5067_RS03895 Genome accession   NZ_CP043342
Coordinates   799561..800541 (+) Length   326 a.a.
NCBI ID   WP_119936199.1    Uniprot ID   -
Organism   Enterobacter huaxiensis strain 090008     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794561..805541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5067_RS03855 (D5067_0003880) - 794628..794954 (+) 327 WP_028014375.1 YggL family protein -
  D5067_RS03860 (D5067_0003885) - 795011..795727 (+) 717 WP_119936204.1 DUF2884 domain-containing protein -
  D5067_RS03865 (D5067_0003890) - 795803..796165 (-) 363 WP_119936203.1 endonuclease domain-containing protein -
  D5067_RS03870 (D5067_0003895) hemW 796229..797371 (-) 1143 WP_119936202.1 radical SAM family heme chaperone HemW -
  D5067_RS03875 (D5067_0003900) - 797364..797957 (-) 594 WP_119936201.1 XTP/dITP diphosphatase -
  D5067_RS03880 (D5067_0003905) yggU 797961..798257 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  D5067_RS03885 (D5067_0003910) - 798254..798820 (-) 567 WP_023333348.1 YggT family protein -
  D5067_RS03890 (D5067_0003915) - 798842..799543 (-) 702 WP_119936200.1 YggS family pyridoxal phosphate-dependent enzyme -
  D5067_RS03895 (D5067_0003920) pilT 799561..800541 (+) 981 WP_119936199.1 type IV pilus twitching motility protein PilT Machinery gene
  D5067_RS03900 (D5067_0003925) ruvX 800552..800968 (-) 417 WP_014171567.1 Holliday junction resolvase RuvX -
  D5067_RS03905 (D5067_0003930) - 800968..801528 (-) 561 WP_119936198.1 YqgE/AlgH family protein -
  D5067_RS03910 (D5067_0003935) gshB 801603..802550 (-) 948 WP_119936197.1 glutathione synthase -
  D5067_RS03915 (D5067_0003940) rsmE 802570..803301 (-) 732 WP_119936196.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  D5067_RS03920 (D5067_0003945) endA 803354..804061 (-) 708 WP_119936195.1 deoxyribonuclease I -
  D5067_RS03925 (D5067_0003950) - 804156..804653 (-) 498 WP_119936194.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35632.64 Da        Isoelectric Point: 6.2330

>NTDB_id=382966 D5067_RS03895 WP_119936199.1 799561..800541(+) (pilT) [Enterobacter huaxiensis strain 090008]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVDALLKAWLNDEQQGAWWANGQVDFAVTLAGNQRLRG
SAFTHMKGVSIVLRLLPLACPQLSTLGVPRAIPELLSNDSGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREVGQHSPSFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=382966 D5067_RS03895 WP_119936199.1 799561..800541(+) (pilT) [Enterobacter huaxiensis strain 090008]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCTCCGCG
CTGGCGCAGATTAGGCCGGCTCGAACCGGCACCCTTTCCGCCCCCCGATGTCGACGCGCTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGGGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACCCTCGCAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCACATGAAGGGCGTGTCGATCGTCTTGCGACTGTTGCCGCTCGCCTGTCCACAGCTTTCAACCTTGGG
TGTCCCCCGGGCGATCCCGGAGCTGCTCTCAAATGACAGCGGCCTGATACTGGTCACGGGCGCGACCGGCAGCGGTAAAT
CGACCACCCTCGCCGCTATGGTCGATTTCCTCAACCACCATACTGACGGGCATATTCTGACGCTCGAAGATCCGGTTGAG
TTTATGTATCAGAGTGAGCGTTGCCTGATCCAGCAGCGGGAGGTAGGCCAGCATAGCCCGTCCTTTGCCGAGGCGCTGCG
TGGTGCGCTGCGTGAAGATCCGGATGTCATCCTGCTGGGTGAGCTACGCGACAGTGAGACGATCCGCCTGGCGCTGACGG
CGGCAGAGACCGGGCATCTGGTGCTCGCGACCCTGCACACGCGCGGAGCGTCGCAGGCCATAGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGGCAGCCTGAGAGCGGTTCTGGCGCAAAAGCTGCGTCA
GGACGTACAGGGCGGGCGCGTGGCGCTTTATGAACTGTTGGTGAACACCCCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACGTGGCAGCTTCCCGGCATCATTCAGACCGGCCAGCAGGCGGGAATGCAAAACTTTGAGCAGAGCCTGGCAGAGCGG
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.653

99.08

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment