Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TTHE_RS06345 Genome accession   NC_014410
Coordinates   1300784..1301836 (+) Length   350 a.a.
NCBI ID   WP_013297759.1    Uniprot ID   A0A231VJ17
Organism   Thermoanaerobacterium thermosaccharolyticum DSM 571     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1295784..1306836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTHE_RS06325 (Tthe_1274) gdhA 1296061..1297395 (+) 1335 WP_013297755.1 NADP-specific glutamate dehydrogenase -
  TTHE_RS06330 (Tthe_1275) - 1297529..1298014 (+) 486 WP_041587440.1 YqeG family HAD IIIA-type phosphatase -
  TTHE_RS06335 (Tthe_1276) aroE 1298021..1298872 (+) 852 WP_013297757.1 shikimate dehydrogenase -
  TTHE_RS06340 (Tthe_1277) - 1299029..1300723 (+) 1695 WP_013297758.1 GspE/PulE family protein -
  TTHE_RS06345 (Tthe_1278) pilT 1300784..1301836 (+) 1053 WP_013297759.1 type IV pilus twitching motility protein PilT Machinery gene
  TTHE_RS06350 (Tthe_1279) - 1301866..1303080 (+) 1215 WP_013297760.1 type II secretion system F family protein -
  TTHE_RS06355 (Tthe_1280) - 1303109..1303501 (+) 393 WP_013297761.1 type II secretion system protein -
  TTHE_RS06360 (Tthe_1281) - 1303551..1304306 (+) 756 WP_013297762.1 A24 family peptidase -
  TTHE_RS06365 (Tthe_1282) - 1304347..1304814 (+) 468 WP_013297763.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TTHE_RS06370 (Tthe_1283) - 1304825..1305466 (+) 642 WP_013297764.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TTHE_RS06375 (Tthe_1284) - 1305457..1305918 (+) 462 WP_013297765.1 type II secretion system protein -
  TTHE_RS06380 (Tthe_1285) - 1305908..1306483 (+) 576 WP_013297766.1 pilus assembly PilX N-terminal domain-containing protein -
  TTHE_RS06385 (Tthe_1286) - 1306498..1306773 (+) 276 WP_013297767.1 late competence development ComFB family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38685.95 Da        Isoelectric Point: 6.3956

>NTDB_id=38125 TTHE_RS06345 WP_013297759.1 1300784..1301836(+) (pilT) [Thermoanaerobacterium thermosaccharolyticum DSM 571]
MKTIELLTLVVKNGASDLHITVGVPPVLRINGQLVKLDLPQLTPQDTEEITRDLLSSDELKKLEETGDIDLSYSVQGLGR
FRINAYKQRGTYSLAIRSVALRIPTIDELGLPEVIKELSLKTRGLILVTGPTGSGKSTTLASMIDLINDVRNCHILTLED
PIEYLHKHKKSIVNQREIGHDALSYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGSAKTIDRI
IDVFPPYQQQQIKVQLSNVLEGIISQQLLPKLDNSGRVVAVEVMIATPAIRNLIREGKSFQIQSMVQTGSKFGMMTMDMS
ISQLLKKNLISMDDALTYCVDRENFSKLAL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=38125 TTHE_RS06345 WP_013297759.1 1300784..1301836(+) (pilT) [Thermoanaerobacterium thermosaccharolyticum DSM 571]
ATGAAAACAATTGAACTATTGACGCTAGTTGTAAAAAACGGTGCATCTGATTTACATATAACGGTAGGTGTTCCTCCTGT
ATTAAGAATAAATGGGCAACTTGTAAAACTCGATTTGCCACAGTTGACGCCGCAAGATACGGAAGAAATCACAAGAGATT
TACTTTCGAGCGATGAACTAAAAAAACTTGAAGAGACAGGAGACATAGATCTTTCGTATTCTGTACAGGGGCTTGGAAGA
TTTAGAATAAATGCCTACAAACAGAGAGGTACATATAGTCTTGCAATAAGGTCTGTTGCATTGCGAATACCGACTATTGA
TGAATTGGGACTTCCAGAGGTTATAAAAGAGCTTTCATTGAAAACTCGTGGCCTTATACTTGTAACAGGTCCAACAGGCA
GCGGGAAATCCACAACACTTGCATCTATGATTGATCTGATAAATGATGTGCGAAATTGCCATATATTGACTCTTGAAGAT
CCGATAGAGTATCTACATAAACACAAAAAAAGCATAGTAAATCAAAGAGAGATAGGTCACGATGCATTGTCTTATGCTAG
TGCACTAAGGGCAGCTTTAAGGGAAGATCCTGATGTCATACTTGTAGGTGAGATGAGAGATTTAGAAACAATACAGATAG
CTATAACTGCTGCTGAAACAGGACATCTCGTATTATCTACATTGCATACTATAGGCTCTGCTAAGACTATTGACAGAATT
ATAGATGTATTTCCTCCGTATCAACAACAGCAGATAAAAGTACAGCTTTCGAATGTGTTAGAAGGCATAATTTCACAGCA
GTTGCTGCCTAAACTTGATAATTCAGGACGTGTTGTGGCTGTTGAGGTAATGATAGCGACTCCTGCCATAAGGAATTTGA
TTAGAGAAGGTAAATCATTCCAAATCCAATCTATGGTTCAAACAGGAAGTAAATTTGGAATGATGACAATGGATATGTCA
ATATCACAGCTTTTAAAAAAGAATTTGATTTCGATGGATGATGCATTGACATATTGTGTAGACAGGGAAAACTTTTCAAA
ATTGGCATTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A231VJ17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.145

98.571

0.494
  pilT Legionella pneumophila strain Lp02

49.855

98.571

0.491
  pilT Legionella pneumophila strain ERS1305867

49.855

98.571

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

98.571

0.489
  pilT Pseudomonas stutzeri DSM 10701

49.565

98.571

0.489
  pilT Acinetobacter baylyi ADP1

49.275

98.571

0.486
  pilT Acinetobacter baumannii D1279779

48.986

98.571

0.483
  pilT Acinetobacter baumannii strain A118

48.986

98.571

0.483
  pilT Acinetobacter nosocomialis M2

48.986

98.571

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.788

94.286

0.46
  pilT Vibrio cholerae strain A1552

48.788

94.286

0.46
  pilT Neisseria meningitidis 8013

46.11

99.143

0.457
  pilT Neisseria gonorrhoeae MS11

45.376

98.857

0.449
  pilU Vibrio cholerae strain A1552

40

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

40.413

96.857

0.391
  pilU Acinetobacter baylyi ADP1

39.881

96

0.383

Multiple sequence alignment